Restriction map for
pFN21AE3952
(Linear) (Six-base) MAPSORT of: KIEE3952.seq Check: 9928 from: 1 to: 5133
KIEE3952.orf
With 182 enzymes: SgfI *
March 22, 2012 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3199 4965 5133
Size: 3199 1766 168
Fragments arranged by size:
3199 1766 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 857 953 1227 2941 3260 3482 4000
Size: 570 287 96 274 1714 319 222 518
Cuts at: 4000 4618 4915 5133
Size: 618 297 218
Fragments arranged by size:
1714 618 570 518 319 297 287 274
222 218 96
AfeI AGC'GCT
Cuts at: 0 4606 5133
Size: 4606 527
AflII C'TTAA_G
Cuts at: 0 1801 5133
Size: 1801 3332
AflIII A'CryG_T
Cuts at: 0 2293 2969 5133
Size: 2293 676 2164
Fragments arranged by size:
2293 2164 676
AgeI A'CCGG_T
Cuts at: 0 1137 5133
Size: 1137 3996
AhdI GACnn_n'nnGTC
Cuts at: 0 2561 5133
Size: 2561 2572
AleI CACnn'nnGTG
Cuts at: 0 1845 2104 2149 5133
Size: 1845 259 45 2984
Fragments arranged by size:
2984 1845 259 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1873 5133
Size: 1873 3260
AlwNI CAG_nnn'CTG
Cuts at: 0 322 862 904 1344 1698 2173 2484 3006
Size: 322 540 42 440 354 475 311 522
Cuts at: 3006 3919 4995 5133
Size: 913 1076 138
Fragments arranged by size:
1076 913 540 522 475 440 354 322
311 138 42
ApaI G_GGCC'C
Cuts at: 0 4856 5133
Size: 4856 277
ApaLI G'TGCA_C
Cuts at: 0 31 1948 2641 5133
Size: 31 1917 693 2492
Fragments arranged by size:
2492 1917 693 31
ApoI r'AATT_y
Cuts at: 0 4233 5113 5133
Size: 4233 880 20
Fragments arranged by size:
4233 880 20
AvaI C'yCGr_G
Cuts at: 0 212 1332 1489 2071 2094 3266 3893 4394
Size: 212 1120 157 582 23 1172 627 501
Cuts at: 4394 4560 4905 5133
Size: 166 345 228
Fragments arranged by size:
1172 1120 627 582 501 345 228 212
166 157 23
AvrII C'CTAG_G
Cuts at: 0 5097 5133
Size: 5097 36
BanI G'GyrC_C
Cuts at: 0 336 846 1140 1278 1756 2143 2287 2387
Size: 336 510 294 138 478 387 144 100
Cuts at: 2387 2571 3878 5133
Size: 184 1307 1255
Fragments arranged by size:
1307 1255 510 478 387 336 294 184
144 138 100
BanII G_rGCy'C
Cuts at: 0 185 365 1496 1723 2044 2249 2680 3097
Size: 185 180 1131 227 321 205 431 417
Cuts at: 3097 3894 4747 4856 5133
Size: 797 853 109 277
Fragments arranged by size:
1131 853 797 431 417 321 277 227
205 185 180 109
BbeI G_GCGC'C
Cuts at: 0 2575 5133
Size: 2575 2558
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 3168 3738 4002 4055 4105 5133
Size: 272 2896 570 264 53 50 1028
Fragments arranged by size:
2896 1028 570 272 264 53 50
BbvCI CC'TCA_GC
Cuts at: 0 323 2324 2776 5133
Size: 323 2001 452 2357
Fragments arranged by size:
2357 2001 452 323
BciVI GTATCCnnnnn_n'
Cuts at: 0 4674 5133
Size: 4674 459
BclI T'GATC_A
Cuts at: 0 2126 2262 4617 5133
Size: 2126 136 2355 516
Fragments arranged by size:
2355 2126 516 136
BfrBI ATG'CAT
Cuts at: 0 658 1513 5133
Size: 658 855 3620
Fragments arranged by size:
3620 855 658
BglI GCCn_nnn'nGGC
Cuts at: 0 217 2580 3262 5133
Size: 217 2363 682 1871
Fragments arranged by size:
2363 1871 682 217
BlpI GC'TnA_GC
Cuts at: 0 1722 3788 3862 5133
Size: 1722 2066 74 1271
Fragments arranged by size:
2066 1722 1271 74
Bme1580I G_kGCm'C
Cuts at: 0 35 257 341 641 797 965 1067 1952
Size: 35 222 84 300 156 168 102 885
Cuts at: 1952 2284 2645 4856 5133
Size: 332 361 2211 277
Fragments arranged by size:
2211 885 361 332 300 277 222 168
156 102 84 35
BmgBI CAC'GTC
Cuts at: 0 3072 5133
Size: 3072 2061
BmrI ACTGGGnnnn_n'
Cuts at: 0 682 916 1706 1745 2161 2555 2798 3208
Size: 682 234 790 39 416 394 243 410
Cuts at: 3208 3449 3564 3819 4034 5133
Size: 241 115 255 215 1099
Fragments arranged by size:
1099 790 682 416 410 394 255 243
241 234 215 115 39
BmtI G_CTAG'C
Cuts at: 0 1483 5133
Size: 1483 3650
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 1851 2268 5133
Size: 379 1472 417 2865
Fragments arranged by size:
2865 1472 417 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 761 881 1022 1157 1582 2342 2613
Size: 563 198 120 141 135 425 760 271
Cuts at: 2613 3081 3379 3560 3927 4309 4447 4525 4968
Size: 468 298 181 367 382 138 78 443
Cuts at: 4968 5036 5133
Size: 68 97
Fragments arranged by size:
760 563 468 443 425 382 367 298
271 198 181 141 138 135 120 97
78 68
Bpu10I CC'TnA_GC
Cuts at: 0 323 343 753 1274 2324 2776 3020 3251
Size: 323 20 410 521 1050 452 244 231
Cuts at: 3251 5133
Size: 1882
Fragments arranged by size:
1882 1050 521 452 410 323 244 231
20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 5133
Size: 349 4784
BsaI GGTCTCn'nnnn_
Cuts at: 0 1997 4501 5133
Size: 1997 2504 632
Fragments arranged by size:
2504 1997 632
BsaAI yAC'GTr
Cuts at: 0 2151 2294 5133
Size: 2151 143 2839
Fragments arranged by size:
2839 2151 143
BsaBI GATnn'nnATC
Cuts at: 0 4346 5133
Size: 4346 787
BsaHI Gr'CG_yC
Cuts at: 0 2515 2572 5133
Size: 2515 57 2561
Fragments arranged by size:
2561 2515 57
BsaWI w'CCGG_w
Cuts at: 0 53 1005 1137 1594 3419 3545 5133
Size: 53 952 132 457 1825 126 1588
Fragments arranged by size:
1825 1588 952 457 132 126 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 536 566 1779 1809 3556 3586 5078 5108
Size: 536 30 1213 30 1747 30 1492 30
Cuts at: 5108 5133
Size: 25
Fragments arranged by size:
1747 1492 1213 536 30 30 30 30
25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 121 923 1787 1960 3781 3961 3964
Size: 94 27 802 864 173 1821 180 3
Cuts at: 3964 3967 3979 3982 4098 4789 5037 5133
Size: 3 12 3 116 691 248 96
Fragments arranged by size:
1821 864 802 691 248 180 173 116
96 94 27 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 284 304 1155 1610 3783 5133
Size: 284 20 851 455 2173 1350
Fragments arranged by size:
2173 1350 851 455 284 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1208 1566 1601 1869 2105 5133
Size: 1130 78 358 35 268 236 3028
Fragments arranged by size:
3028 1130 358 268 236 78 35
BsiHKAI G_wGCw'C
Cuts at: 0 35 93 185 1496 1723 1952 2101 2645
Size: 35 58 92 1311 227 229 149 544
Cuts at: 2645 3518 3667 4127 4433 4747 5133
Size: 873 149 460 306 314 386
Fragments arranged by size:
1311 873 544 460 386 314 306 229
227 149 149 92 58 35
BsmI GAATG_Cn'
Cuts at: 0 658 5133
Size: 658 4475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 350 2522 4293 4754 5133
Size: 350 2172 1771 461 379
Fragments arranged by size:
2172 1771 461 379 350
Bsp1286I G_dGCh'C
Cuts at: 0 35 93 185 257 341 365 641 797
Size: 35 58 92 72 84 24 276 156
Cuts at: 797 965 1067 1496 1723 1952 2044 2101 2249
Size: 168 102 429 227 229 92 57 148
Cuts at: 2249 2284 2645 2680 3097 3518 3667 3894 4127
Size: 35 361 35 417 421 149 227 233
Cuts at: 4127 4433 4747 4856 5133
Size: 306 314 109 277
Fragments arranged by size:
429 421 417 361 314 306 277 276
233 229 227 227 168 156 149 148
109 102 92 92 84 72 58 57
35 35 35 24
BspEI T'CCGG_A
Cuts at: 0 3419 5133
Size: 3419 1714
BspHI T'CATG_A
Cuts at: 0 4479 5133
Size: 4479 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1460 3014 3199 4965 5133
Size: 1460 1554 185 1766 168
Fragments arranged by size:
1766 1554 1460 185 168
BsrBI CCG'CTC
Cuts at: 0 18 5133
Size: 18 5115
BsrDI GCAATG_nn'
Cuts at: 0 213 1900 2028 2366 5133
Size: 213 1687 128 338 2767
Fragments arranged by size:
2767 1687 338 213 128
BsrFI r'CCGG_y
Cuts at: 0 1137 2014 2427 5133
Size: 1137 877 413 2706
Fragments arranged by size:
2706 1137 877 413
BssSI C'ACGA_G
Cuts at: 0 3598 4467 5133
Size: 3598 869 666
Fragments arranged by size:
3598 869 666
BstBI TT'CG_AA
Cuts at: 0 2832 5133
Size: 2832 2301
BstEII G'GTnAC_C
Cuts at: 0 573 3771 5133
Size: 573 3198 1362
Fragments arranged by size:
3198 1362 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 136 3414 4278 4581 5133
Size: 136 3278 864 303 552
Fragments arranged by size:
3278 864 552 303 136
BstYI r'GATC_y
Cuts at: 0 139 3132 4172 4629 4910 5032 5133
Size: 139 2993 1040 457 281 122 101
Fragments arranged by size:
2993 1040 457 281 139 122 101
Bsu36I CC'TnA_GG
Cuts at: 0 2276 2654 2849 4132 5133
Size: 2276 378 195 1283 1001
Fragments arranged by size:
2276 1283 1001 378 195
BtgI C'CryG_G
Cuts at: 0 2420 2665 3413 4848 5133
Size: 2420 245 748 1435 285
Fragments arranged by size:
2420 1435 748 285 245
BtsI GCAGTG_nn'
Cuts at: 0 240 1393 4458 5133
Size: 240 1153 3065 675
Fragments arranged by size:
3065 1153 675 240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2574 2609 5133
Size: 2574 35 2524
Fragments arranged by size:
2574 2524 35
DraI TTT'AAA
Cuts at: 0 4082 5133
Size: 4082 1051
DraIII CAC_nnn'GTG
Cuts at: 0 1384 5133
Size: 1384 3749
EaeI y'GGCC_r
Cuts at: 0 1388 2159 2960 2990 4583 5133
Size: 1388 771 801 30 1593 550
Fragments arranged by size:
1593 1388 801 771 550 30
EarI CTCTTCn'nnn_
Cuts at: 0 81 3377 3758 3956 3976 4000 4208 4289
Size: 81 3296 381 198 20 24 208 81
Cuts at: 4289 4817 4882 5133
Size: 528 65 251
Fragments arranged by size:
3296 528 381 251 208 198 81 81
65 24 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4077 5133
Size: 4077 1056
EcoICRI GAG'CTC
Cuts at: 0 183 1494 1721 4745 5133
Size: 183 1311 227 3024 388
Fragments arranged by size:
3024 1311 388 227 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 570 761 857 881 953 1022 1157
Size: 563 7 191 96 24 72 69 135
Cuts at: 1157 1227 1582 2342 2613 2941 3081 3260 3379
Size: 70 355 760 271 328 140 179 119
Cuts at: 3379 3482 3560 3927 4000 4309 4447 4525 4618
Size: 103 78 367 73 309 138 78 93
Cuts at: 4618 4915 4968 5036 5133
Size: 297 53 68 97
Fragments arranged by size:
760 563 367 355 328 309 297 271
191 179 140 138 135 119 103 97
96 93 78 78 73 72 70 69
68 53 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4381 5133
Size: 4381 752
EcoO109I rG'GnC_Cy
Cuts at: 0 327 560 1629 1965 2091 2481 2697 2792
Size: 327 233 1069 336 126 390 216 95
Cuts at: 2792 2845 2899 3972 4853 5133
Size: 53 54 1073 881 280
Fragments arranged by size:
1073 1069 881 390 336 327 280 233
216 126 95 54 53
EcoRI G'AATT_C
Cuts at: 0 5113 5133
Size: 5113 20
FspI TGC'GCA
Cuts at: 0 4457 5133
Size: 4457 676
HaeII r_GCGC'y
Cuts at: 0 389 784 2575 3111 4608 5133
Size: 389 395 1791 536 1497 525
Fragments arranged by size:
1791 1497 536 525 395 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 1821 1853 1858 1890 2547 2579
Size: 94 32 1695 32 5 32 657 32
Cuts at: 2579 3321 3353 3556 3588 4609 4641 4734 4766
Size: 742 32 203 32 1021 32 93 32
Cuts at: 4766 5017 5049 5133
Size: 251 32 84
Fragments arranged by size:
1695 1021 742 657 251 203 94 93
84 32 32 32 32 32 32 32
32 32 5
HincII GTy'rAC
Cuts at: 0 113 456 1940 5133
Size: 113 343 1484 3193
Fragments arranged by size:
3193 1484 343 113
HindIII A'AGCT_T
Cuts at: 0 367 1894 4897 5133
Size: 367 1527 3003 236
Fragments arranged by size:
3003 1527 367 236
KasI G'GCGC_C
Cuts at: 0 2571 5133
Size: 2571 2562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 483 1000 1102 1708 1907 4673 5133
Size: 174 309 517 102 606 199 2766 460
Fragments arranged by size:
2766 606 517 460 309 199 174 102
MscI TGG'CCA
Cuts at: 0 1390 2161 2962 2992 4585 5133
Size: 1390 771 801 30 1593 548
Fragments arranged by size:
1593 1390 801 771 548 30
MslI CAynn'nnrTG
Cuts at: 0 439 1845 1933 2104 2149 2833 2968 4948
Size: 439 1406 88 171 45 684 135 1980
Cuts at: 4948 5133
Size: 185
Fragments arranged by size:
1980 1406 684 439 185 171 135 88
45
MspA1I CmG'CkG
Cuts at: 0 26 1042 1207 1344 1818 2553 4059 4850
Size: 26 1016 165 137 474 735 1506 791
Cuts at: 4850 4992 5133
Size: 142 141
Fragments arranged by size:
1506 1016 791 735 474 165 142 141
137 26
NaeI GCC'GGC
Cuts at: 0 2429 5133
Size: 2429 2704
NarI GG'CG_CC
Cuts at: 0 2572 5133
Size: 2572 2561
NgoMIV G'CCGG_C
Cuts at: 0 2427 5133
Size: 2427 2706
NheI G'CTAG_C
Cuts at: 0 1479 5133
Size: 1479 3654
NsiI A_TGCA'T
Cuts at: 0 660 1515 5133
Size: 660 855 3618
Fragments arranged by size:
3618 855 660
NspI r_CATG'y
Cuts at: 0 66 2973 5133
Size: 66 2907 2160
Fragments arranged by size:
2907 2160 66
PasI CC'CwG_GG
Cuts at: 0 1633 2374 2904 3488 5133
Size: 1633 741 530 584 1645
Fragments arranged by size:
1645 1633 741 584 530
PciI A'CATG_T
Cuts at: 0 2969 5133
Size: 2969 2164
PflMI CCAn_nnn'nTGG
Cuts at: 0 1344 1369 2671 2958 2982 3118 4119 5133
Size: 1344 25 1302 287 24 136 1001 1014
Fragments arranged by size:
1344 1302 1014 1001 287 136 25 24
PfoI T'CCnGG_A
Cuts at: 0 418 3576 5133
Size: 418 3158 1557
Fragments arranged by size:
3158 1557 418
PmlI CAC'GTG
Cuts at: 0 2294 5133
Size: 2294 2839
PpuMI rG'GwC_Cy
Cuts at: 0 560 1629 2091 2481 2697 2792 5133
Size: 560 1069 462 390 216 95 2341
Fragments arranged by size:
2341 1069 560 462 390 216 95
PshAI GACnn'nnGTC
Cuts at: 0 1463 5133
Size: 1463 3670
PspOMI G'GGCC_C
Cuts at: 0 4852 5133
Size: 4852 281
PspXI vC'TCGA_Gb
Cuts at: 0 3266 4905 5133
Size: 3266 1639 228
Fragments arranged by size:
3266 1639 228
PstI C_TGCA'G
Cuts at: 0 1272 1457 4453 5133
Size: 1272 185 2996 680
Fragments arranged by size:
2996 1272 680 185
PvuII CAG'CTG
Cuts at: 0 1207 1818 2553 4992 5133
Size: 1207 611 735 2439 141
Fragments arranged by size:
2439 1207 735 611 141
SacI G_AGCT'C
Cuts at: 0 185 1496 1723 4747 5133
Size: 185 1311 227 3024 386
Fragments arranged by size:
3024 1311 386 227 185
SacII CC_GC'GG
Cuts at: 0 4851 5133
Size: 4851 282
SanDI GG'GwC_CC
Cuts at: 0 560 1629 2091 2792 5133
Size: 560 1069 462 701 2341
Fragments arranged by size:
2341 1069 701 560 462
SapI GCTCTTCn'nnn_
Cuts at: 0 81 3377 4000 4208 5133
Size: 81 3296 623 208 925
Fragments arranged by size:
3296 925 623 208 81
SbfI CC_TGCA'GG
Cuts at: 0 1457 5133
Size: 1457 3676
SfcI C'TryA_G
Cuts at: 0 8 21 239 1268 1453 3696 4449 4968
Size: 8 13 218 1029 185 2243 753 519
Cuts at: 4968 5133
Size: 165
Fragments arranged by size:
2243 1029 753 519 218 185 165 13
8
SfoI GGC'GCC
Cuts at: 0 2573 5133
Size: 2573 2560
SgrAI Cr'CCGG_yG
Cuts at: 0 1137 5133
Size: 1137 3996
SmaI CCC'GGG
Cuts at: 0 214 4396 5133
Size: 214 4182 737
Fragments arranged by size:
4182 737 214
SmlI C'TyrA_G
Cuts at: 0 364 1801 3266 4905 5133
Size: 364 1437 1465 1639 228
Fragments arranged by size:
1639 1465 1437 364 228
SspI AAT'ATT
Cuts at: 0 2436 5133
Size: 2436 2697
StuI AGG'CCT
Cuts at: 0 5013 5133
Size: 5013 120
StyI C'CwwG_G
Cuts at: 0 173 938 980 1673 2396 3053 3407 3611
Size: 173 765 42 693 723 657 354 204
Cuts at: 3611 4955 5097 5133
Size: 1344 142 36
Fragments arranged by size:
1344 765 723 693 657 354 204 173
142 42 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 1164 1200 1242 1624 2094 2491 3838
Size: 451 713 36 42 382 470 397 1347
Cuts at: 3838 4420 5133
Size: 582 713
Fragments arranged by size:
1347 713 713 582 470 451 397 382
42 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 536 568 717 749 3113 3145 5133
Size: 536 32 149 32 2364 32 1988
Fragments arranged by size:
2364 1988 536 149 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1008 2014 2911 5133
Size: 1008 1006 897 2222
Fragments arranged by size:
2222 1008 1006 897
XhoI C'TCGA_G
Cuts at: 0 3266 4905 5133
Size: 3266 1639 228
Fragments arranged by size:
3266 1639 228
XmaI C'CCGG_G
Cuts at: 0 212 4394 5133
Size: 212 4182 739
Fragments arranged by size:
4182 739 212
XmnI GAAnn'nnTTC
Cuts at: 0 517 5133
Size: 517 4616
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI SbfI SfcI
SfoI SgrAI SmaI SmlI SspI StuI StyI TaqII
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AloI AscI AseI AsiSI
BaeI BamHI BcgI BglII BsiEI BsiWI BsrGI BssHII
BstAPI BstZ17I BtgZI ClaI DrdI EagI EcoRV FalI
FseI FspAI HpaI KpnI MfeI MluI NcoI NdeI
NotI NruI PacI PmeI PpiI PsiI PsrI PvuI
RsrII SalI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI SwaI TatI Tth111I XbaI ZraI