Restriction map for
pFN21AE3951
(Linear) (Six-base) MAPSORT of: KIEE3951.seq Check: 3791 from: 1 to: 2271
KIEE3951.orf
With 182 enzymes: SgfI *
July 3, 2012 18:21 ..
AflIII A'CryG_T
Cuts at: 0 482 1523 2271
Size: 482 1041 748
Fragments arranged by size:
1041 748 482
AleI CACnn'nnGTG
Cuts at: 0 1015 2271
Size: 1015 1256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 143 2271
Size: 143 2128
AlwNI CAG_nnn'CTG
Cuts at: 0 1662 2008 2271
Size: 1662 346 263
Fragments arranged by size:
1662 346 263
ApaI G_GGCC'C
Cuts at: 0 163 182 494 1850 2271
Size: 163 19 312 1356 421
Fragments arranged by size:
1356 421 312 163 19
AscI GG'CGCG_CC
Cuts at: 0 192 2271
Size: 192 2079
AvaI C'yCGr_G
Cuts at: 0 82 181 208 358 387 420 988 1180
Size: 82 99 27 150 29 33 568 192
Cuts at: 1180 1194 1861 2271
Size: 14 667 410
Fragments arranged by size:
667 568 410 192 150 99 82 33
29 27 14
BamHI G'GATC_C
Cuts at: 0 1995 2271
Size: 1995 276
BanI G'GyrC_C
Cuts at: 0 35 63 168 391 416 609 625 652
Size: 35 28 105 223 25 193 16 27
Cuts at: 652 757 806 993 1417 2271
Size: 105 49 187 424 854
Fragments arranged by size:
854 424 223 193 187 105 105 49
35 28 27 25 16
BanII G_rGCy'C
Cuts at: 0 10 163 182 359 494 512 725 1037
Size: 10 153 19 177 135 18 213 312
Cuts at: 1037 1444 1711 1850 2074 2271
Size: 407 267 139 224 197
Fragments arranged by size:
407 312 267 224 213 197 177 153
139 135 19 18 10
BbeI G_GCGC'C
Cuts at: 0 39 67 395 613 629 656 761 810
Size: 39 28 328 218 16 27 105 49
Cuts at: 810 1421 2271
Size: 611 850
Fragments arranged by size:
850 611 328 218 105 49 39 28
27 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 2069 2271
Size: 420 1649 202
Fragments arranged by size:
1649 420 202
BbvCI CC'TCA_GC
Cuts at: 0 584 1201 2271
Size: 584 617 1070
Fragments arranged by size:
1070 617 584
BclI T'GATC_A
Cuts at: 0 272 2271
Size: 272 1999
BglI GCCn_nnn'nGGC
Cuts at: 0 1196 2271
Size: 1196 1075
BlpI GC'TnA_GC
Cuts at: 0 4 1406 2271
Size: 4 1402 865
Fragments arranged by size:
1402 865 4
Bme1580I G_kGCm'C
Cuts at: 0 163 182 494 996 1572 1607 1850 2271
Size: 163 19 312 502 576 35 243 421
Fragments arranged by size:
576 502 421 312 243 163 35 19
BmgBI CAC'GTC
Cuts at: 0 483 540 1224 1538 2271
Size: 483 57 684 314 733
Fragments arranged by size:
733 684 483 314 57
Bpu10I CC'TnA_GC
Cuts at: 0 584 1201 1870 1946 2271
Size: 584 617 669 76 325
Fragments arranged by size:
669 617 584 325 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 2271
Size: 709 1562
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 2271
Size: 526 1745
BsaAI yAC'GTr
Cuts at: 0 438 1050 2271
Size: 438 612 1221
Fragments arranged by size:
1221 612 438
BsaBI GATnn'nnATC
Cuts at: 0 271 1963 2271
Size: 271 1692 308
Fragments arranged by size:
1692 308 271
BsaHI Gr'CG_yC
Cuts at: 0 36 64 392 610 626 653 758 807
Size: 36 28 328 218 16 27 105 49
Cuts at: 807 1418 2271
Size: 611 853
Fragments arranged by size:
853 611 328 218 105 49 36 28
27 16
BsaWI w'CCGG_w
Cuts at: 0 999 2271
Size: 999 1272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 1304 1334 2163 2193 2271
Size: 1045 30 229 30 829 30 78
Fragments arranged by size:
1045 829 229 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 520 601 1901 1966 2116 2271
Size: 517 3 81 1300 65 150 155
Fragments arranged by size:
1300 517 155 150 81 65 3
BseYI C'CCAG_C
Cuts at: 0 474 1227 1308 1839 1890 2031 2271
Size: 474 753 81 531 51 141 240
Fragments arranged by size:
753 531 474 240 141 81 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 2271
Size: 451 1820
BsiHKAI G_wGCw'C
Cuts at: 0 10 443 512 725 1379 2181 2271
Size: 10 433 69 213 654 802 90
Fragments arranged by size:
802 654 433 213 90 69 10
BsiWI C'GTAC_G
Cuts at: 0 434 2271
Size: 434 1837
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 292 1021 2271
Size: 24 268 729 1250
Fragments arranged by size:
1250 729 268 24
Bsp1286I G_dGCh'C
Cuts at: 0 10 163 182 359 443 494 512 725
Size: 10 153 19 177 84 51 18 213
Cuts at: 725 996 1037 1379 1444 1572 1607 1711 1850
Size: 271 41 342 65 128 35 104 139
Cuts at: 1850 2074 2181 2271
Size: 224 107 90
Fragments arranged by size:
342 271 224 213 177 153 139 128
107 104 90 84 65 51 41 35
19 18 10
BspEI T'CCGG_A
Cuts at: 0 999 2271
Size: 999 1272
BsrBI CCG'CTC
Cuts at: 0 308 712 1882 2271
Size: 308 404 1170 389
Fragments arranged by size:
1170 404 389 308
BsrFI r'CCGG_y
Cuts at: 0 60 188 216 343 413 457 566 2271
Size: 60 128 28 127 70 44 109 1705
Fragments arranged by size:
1705 128 127 109 70 60 44 28
BssHII G'CGCG_C
Cuts at: 0 192 2271
Size: 192 2079
BssSI C'ACGA_G
Cuts at: 0 2020 2271
Size: 2020 251
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1662 2008 2271
Size: 1662 346 263
Fragments arranged by size:
1662 346 263
BstEII G'GTnAC_C
Cuts at: 0 966 1494 2271
Size: 966 528 777
Fragments arranged by size:
966 777 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 2271
Size: 873 1398
BstYI r'GATC_y
Cuts at: 0 1977 1995 2271
Size: 1977 18 276
Fragments arranged by size:
1977 276 18
BtgI C'CryG_G
Cuts at: 0 30 174 227 620 1646 1764 1811 2091
Size: 30 144 53 393 1026 118 47 280
Cuts at: 2091 2252 2271
Size: 161 19
Fragments arranged by size:
1026 393 280 161 144 118 53 47
30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 559 1575 2271
Size: 559 1016 696
Fragments arranged by size:
1016 696 559
BtsI GCAGTG_nn'
Cuts at: 0 468 2185 2271
Size: 468 1717 86
Fragments arranged by size:
1717 468 86
DraIII CAC_nnn'GTG
Cuts at: 0 803 1293 2271
Size: 803 490 978
Fragments arranged by size:
978 803 490
EaeI y'GGCC_r
Cuts at: 0 224 341 1160 1643 1649 1826 2094 2271
Size: 224 117 819 483 6 177 268 177
Fragments arranged by size:
819 483 268 224 177 177 117 6
EarI CTCTTCn'nnn_
Cuts at: 0 841 895 2271
Size: 841 54 1376
Fragments arranged by size:
1376 841 54
EcoICRI GAG'CTC
Cuts at: 0 8 510 723 2271
Size: 8 502 213 1548
Fragments arranged by size:
1548 502 213 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 2271
Size: 499 1772
EcoO109I rG'GnC_Cy
Cuts at: 0 160 491 843 1832 1846 1847 2215 2271
Size: 160 331 352 989 14 1 368 56
Fragments arranged by size:
989 368 352 331 160 56 14 1
EcoRV GAT'ATC
Cuts at: 0 2264 2271
Size: 2264 7
FseI GG_CCGG'CC
Cuts at: 0 347 2271
Size: 347 1924
FspI TGC'GCA
Cuts at: 0 131 319 1096 1267 2271
Size: 131 188 777 171 1004
Fragments arranged by size:
1004 777 188 171 131
FspAI rTGC'GCAy
Cuts at: 0 1096 2271
Size: 1096 1175
HaeII r_GCGC'y
Cuts at: 0 39 67 395 595 613 629 656 732
Size: 39 28 328 200 18 16 27 76
Cuts at: 732 761 810 835 1421 1564 2271
Size: 29 49 25 586 143 707
Fragments arranged by size:
707 586 328 200 143 76 49 39
29 28 27 25 18 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 1304 1336 2271
Size: 835 32 437 32 935
Fragments arranged by size:
935 835 437 32 32
HincII GTy'rAC
Cuts at: 0 1617 2271
Size: 1617 654
HindIII A'AGCT_T
Cuts at: 0 2223 2271
Size: 2223 48
KasI G'GCGC_C
Cuts at: 0 35 63 391 609 625 652 757 806
Size: 35 28 328 218 16 27 105 49
Cuts at: 806 1417 2271
Size: 611 854
Fragments arranged by size:
854 611 328 218 105 49 35 28
27 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 2271
Size: 1469 802
MscI TGG'CCA
Cuts at: 0 1162 1645 1651 2271
Size: 1162 483 6 620
Fragments arranged by size:
1162 620 483 6
MslI CAynn'nnrTG
Cuts at: 0 1015 1594 1600 1816 2271
Size: 1015 579 6 216 455
Fragments arranged by size:
1015 579 455 216 6
MspA1I CmG'CkG
Cuts at: 0 32 105 176 260 622 812 1772 1843
Size: 32 73 71 84 362 190 960 71
Cuts at: 1843 2008 2254 2271
Size: 165 246 17
Fragments arranged by size:
960 362 246 190 165 84 73 71
71 32 17
NaeI GCC'GGC
Cuts at: 0 62 218 345 2271
Size: 62 156 127 1926
Fragments arranged by size:
1926 156 127 62
NarI GG'CG_CC
Cuts at: 0 36 64 392 610 626 653 758 807
Size: 36 28 328 218 16 27 105 49
Cuts at: 807 1418 2271
Size: 611 853
Fragments arranged by size:
853 611 328 218 105 49 36 28
27 16
NcoI C'CATG_G
Cuts at: 0 227 1646 1811 2271
Size: 227 1419 165 460
Fragments arranged by size:
1419 460 227 165
NgoMIV G'CCGG_C
Cuts at: 0 60 216 343 2271
Size: 60 156 127 1928
Fragments arranged by size:
1928 156 127 60
NruI TCG'CGA
Cuts at: 0 578 2271
Size: 578 1693
NspI r_CATG'y
Cuts at: 0 1527 1632 2271
Size: 1527 105 639
Fragments arranged by size:
1527 639 105
PasI CC'CwG_GG
Cuts at: 0 1368 1509 2271
Size: 1368 141 762
Fragments arranged by size:
1368 762 141
PciI A'CATG_T
Cuts at: 0 1523 2271
Size: 1523 748
PflMI CCAn_nnn'nTGG
Cuts at: 0 1538 2271
Size: 1538 733
PfoI T'CCnGG_A
Cuts at: 0 740 2271
Size: 740 1531
PpuMI rG'GwC_Cy
Cuts at: 0 843 2271
Size: 843 1428
PspOMI G'GGCC_C
Cuts at: 0 159 178 490 1846 2271
Size: 159 19 312 1356 425
Fragments arranged by size:
1356 425 312 159 19
PvuII CAG'CTG
Cuts at: 0 2008 2271
Size: 2008 263
SacI G_AGCT'C
Cuts at: 0 10 512 725 2271
Size: 10 502 213 1546
Fragments arranged by size:
1546 502 213 10
SacII CC_GC'GG
Cuts at: 0 33 177 623 2255 2271
Size: 33 144 446 1632 16
Fragments arranged by size:
1632 446 144 33 16
SapI GCTCTTCn'nnn_
Cuts at: 0 841 2271
Size: 841 1430
SfcI C'TryA_G
Cuts at: 0 2100 2271
Size: 2100 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1196 2271
Size: 1196 1075
SfoI GGC'GCC
Cuts at: 0 37 65 393 611 627 654 759 808
Size: 37 28 328 218 16 27 105 49
Cuts at: 808 1419 2271
Size: 611 852
Fragments arranged by size:
852 611 328 218 105 49 37 28
27 16
SmaI CCC'GGG
Cuts at: 0 183 210 389 422 990 1863 2271
Size: 183 27 179 33 568 873 408
Fragments arranged by size:
873 568 408 183 179 33 27
SmlI C'TyrA_G
Cuts at: 0 724 1180 2271
Size: 724 456 1091
Fragments arranged by size:
1091 724 456
StuI AGG'CCT
Cuts at: 0 547 668 976 2271
Size: 547 121 308 1295
Fragments arranged by size:
1295 547 308 121
StyI C'CwwG_G
Cuts at: 0 227 1208 1646 1811 2271
Size: 227 981 438 165 460
Fragments arranged by size:
981 460 438 227 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 550 1809 2126 2271
Size: 550 1259 317 145
Fragments arranged by size:
1259 550 317 145
TatI w'GTAC_w
Cuts at: 0 1631 2271
Size: 1631 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 2163 2195 2271
Size: 1045 32 1086 32 76
Fragments arranged by size:
1086 1045 76 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1539 2271
Size: 1539 732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 1020 1224 1506 2271
Size: 921 99 204 282 765
Fragments arranged by size:
921 765 282 204 99
XhoI C'TCGA_G
Cuts at: 0 1180 2271
Size: 1180 1091
XmaI C'CCGG_G
Cuts at: 0 181 208 387 420 988 1861 2271
Size: 181 27 179 33 568 873 410
Fragments arranged by size:
873 568 410 181 179 33 27
Enzymes that do cut:
AflIII AleI AlfI AlwNI ApaI AscI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
Bme1580I BmgBI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PpuMI PspOMI PvuII SacI SacII SapI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BcgI BciVI BfrBI BglII BmrI BmtI BplI
BpmI BsiEI BsmI BspHI BspMI BsrDI BsrGI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI
Eco57MI EcoRI FalI HpaI KpnI MfeI MluI NdeI
NheI NotI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmnI ZraI