Restriction map for
pFN21AE3949
(Linear) (Six-base) MAPSORT of: KIEE3949.seq Check: 444 from: 1 to: 2208
KIEE3949.orf
With 182 enzymes: SgfI *
March 22, 2012 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 569 2208
Size: 569 1639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 818 1926 2208
Size: 712 106 1108 282
Fragments arranged by size:
1108 712 282 106
AfeI AGC'GCT
Cuts at: 0 353 1132 2208
Size: 353 779 1076
Fragments arranged by size:
1076 779 353
AflII C'TTAA_G
Cuts at: 0 1546 2208
Size: 1546 662
AflIII A'CryG_T
Cuts at: 0 2018 2208
Size: 2018 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1776 2208
Size: 1776 432
AlwNI CAG_nnn'CTG
Cuts at: 0 870 2208
Size: 870 1338
ApaI G_GGCC'C
Cuts at: 0 288 403 2208
Size: 288 115 1805
Fragments arranged by size:
1805 288 115
ApaLI G'TGCA_C
Cuts at: 0 1747 2208
Size: 1747 461
AvaI C'yCGr_G
Cuts at: 0 48 87 280 403 1565 1907 1985 2189
Size: 48 39 193 123 1162 342 78 204
Cuts at: 2189 2208
Size: 19
Fragments arranged by size:
1162 342 204 193 123 78 48 39
19
BamHI G'GATC_C
Cuts at: 0 1206 2208
Size: 1206 1002
BanI G'GyrC_C
Cuts at: 0 93 159 256 683 1928 2025 2153 2184
Size: 93 66 97 427 1245 97 128 31
Cuts at: 2184 2208
Size: 24
Fragments arranged by size:
1245 427 128 97 97 93 66 31
24
BanII G_rGCy'C
Cuts at: 0 65 288 296 403 467 1065 1301 1538
Size: 65 223 8 107 64 598 236 237
Cuts at: 1538 1802 1886 2141 2208
Size: 264 84 255 67
Fragments arranged by size:
598 264 255 237 236 223 107 84
67 65 64 8
BbeI G_GCGC'C
Cuts at: 0 97 163 260 687 1932 2208
Size: 97 66 97 427 1245 276
Fragments arranged by size:
1245 427 276 97 97 66
BbvCI CC'TCA_GC
Cuts at: 0 955 2208
Size: 955 1253
BglI GCCn_nnn'nGGC
Cuts at: 0 145 407 830 1060 2208
Size: 145 262 423 230 1148
Fragments arranged by size:
1148 423 262 230 145
BlpI GC'TnA_GC
Cuts at: 0 1595 2208
Size: 1595 613
Bme1580I G_kGCm'C
Cuts at: 0 288 403 1751 2004 2028 2187 2208
Size: 288 115 1348 253 24 159 21
Fragments arranged by size:
1348 288 253 159 115 24 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 657 688 1738 2208
Size: 372 285 31 1050 470
Fragments arranged by size:
1050 470 372 285 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 1276 2208
Size: 315 961 932
Fragments arranged by size:
961 932 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 691 1366 1493 1627 2208
Size: 652 39 675 127 134 581
Fragments arranged by size:
675 652 581 134 127 39
Bpu10I CC'TnA_GC
Cuts at: 0 59 955 980 1454 2030 2208
Size: 59 896 25 474 576 178
Fragments arranged by size:
896 576 474 178 59 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 1092 2208
Size: 1092 1116
BsaAI yAC'GTr
Cuts at: 0 2021 2208
Size: 2021 187
BsaHI Gr'CG_yC
Cuts at: 0 94 160 257 446 637 684 1929 2208
Size: 94 66 97 189 191 47 1245 279
Fragments arranged by size:
1245 279 191 189 97 94 66 47
BsaWI w'CCGG_w
Cuts at: 0 1948 2208
Size: 1948 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 326 356 2032 2062 2208
Size: 326 30 1676 30 146
Fragments arranged by size:
1676 326 146 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 733 796 820 1825 2101 2104 2208
Size: 1 732 63 24 1005 276 3 104
Fragments arranged by size:
1005 732 276 104 63 24 3 1
BseYI C'CCAG_C
Cuts at: 0 77 207 396 1082 1086 1422 1465 1570
Size: 77 130 189 686 4 336 43 105
Cuts at: 1570 1803 2116 2208
Size: 233 313 92
Fragments arranged by size:
686 336 313 233 189 130 105 92
77 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 2208
Size: 368 1840
BsiEI CG_ry'CG
Cuts at: 0 19 149 167 248 2208
Size: 19 130 18 81 1960
Fragments arranged by size:
1960 130 81 19 18
BsiHKAI G_wGCw'C
Cuts at: 0 1187 1751 2036 2208
Size: 1187 564 285 172
Fragments arranged by size:
1187 564 285 172
BsmBI CGTCTCn'nnnn_
Cuts at: 0 628 2208
Size: 628 1580
Bsp1286I G_dGCh'C
Cuts at: 0 65 288 296 403 467 1065 1187 1301
Size: 65 223 8 107 64 598 122 114
Cuts at: 1301 1538 1751 1802 1886 2004 2028 2036 2141
Size: 237 213 51 84 118 24 8 105
Cuts at: 2141 2187 2208
Size: 46 21
Fragments arranged by size:
598 237 223 213 122 118 114 107
105 84 65 64 51 46 24 21
8 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 485 569 2208
Size: 485 84 1639
Fragments arranged by size:
1639 485 84
BsrBI CCG'CTC
Cuts at: 0 1395 1502 1620 2208
Size: 1395 107 118 588
Fragments arranged by size:
1395 588 118 107
BsrDI GCAATG_nn'
Cuts at: 0 845 2208
Size: 845 1363
BsrFI r'CCGG_y
Cuts at: 0 162 878 2208
Size: 162 716 1330
Fragments arranged by size:
1330 716 162
BssHII G'CGCG_C
Cuts at: 0 135 434 2208
Size: 135 299 1774
Fragments arranged by size:
1774 299 135
BstYI r'GATC_y
Cuts at: 0 510 1206 2208
Size: 510 696 1002
Fragments arranged by size:
1002 696 510
Bsu36I CC'TnA_GG
Cuts at: 0 779 1241 2208
Size: 779 462 967
Fragments arranged by size:
967 779 462
BtgI C'CryG_G
Cuts at: 0 167 1178 1965 2208
Size: 167 1011 787 243
Fragments arranged by size:
1011 787 243 167
BtsI GCAGTG_nn'
Cuts at: 0 703 880 2208
Size: 703 177 1328
Fragments arranged by size:
1328 703 177
DraIII CAC_nnn'GTG
Cuts at: 0 1689 1746 2208
Size: 1689 57 462
Fragments arranged by size:
1689 462 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 81 1762 2208
Size: 81 1681 446
Fragments arranged by size:
1681 446 81
EaeI y'GGCC_r
Cuts at: 0 16 146 164 245 2208
Size: 16 130 18 81 1963
Fragments arranged by size:
1963 130 81 18 16
EagI C'GGCC_G
Cuts at: 0 16 146 164 245 2208
Size: 16 130 18 81 1963
Fragments arranged by size:
1963 130 81 18 16
EarI CTCTTCn'nnn_
Cuts at: 0 731 797 1036 1153 1273 2090 2096 2208
Size: 731 66 239 117 120 817 6 112
Fragments arranged by size:
817 731 239 120 117 112 66 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 442 2208
Size: 346 96 1766
Fragments arranged by size:
1766 346 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 691 712 818 1366 1493 1627 1926
Size: 652 39 21 106 548 127 134 299
Cuts at: 1926 2208
Size: 282
Fragments arranged by size:
652 548 299 282 134 127 106 39
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 838 2208
Size: 838 1370
EcoO109I rG'GnC_Cy
Cuts at: 0 53 105 284 285 310 323 400 485
Size: 53 52 179 1 25 13 77 85
Cuts at: 485 627 775 831 867 1866 2208
Size: 142 148 56 36 999 342
Fragments arranged by size:
999 342 179 148 142 85 77 56
53 52 36 25 13 1
FspI TGC'GCA
Cuts at: 0 384 1556 2208
Size: 384 1172 652
Fragments arranged by size:
1172 652 384
HaeII r_GCGC'y
Cuts at: 0 97 163 175 190 208 260 355 687
Size: 97 66 12 15 18 52 95 332
Cuts at: 687 1134 1703 1932 1998 2208
Size: 447 569 229 66 210
Fragments arranged by size:
569 447 332 229 210 97 95 66
66 52 18 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 326 358 1091 1109 1123 1141 2208
Size: 326 32 733 18 14 18 1067
Fragments arranged by size:
1067 733 326 32 18 18 14
KasI G'GCGC_C
Cuts at: 0 93 159 256 683 1928 2208
Size: 93 66 97 427 1245 280
Fragments arranged by size:
1245 427 280 97 93 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 1385 2208
Size: 1370 15 823
Fragments arranged by size:
1370 823 15
MspA1I CmG'CkG
Cuts at: 0 21 81 520 954 1086 1380 1449 1465
Size: 21 60 439 434 132 294 69 16
Cuts at: 1465 1560 1829 2208
Size: 95 269 379
Fragments arranged by size:
439 434 379 294 269 132 95 69
60 21 16
NaeI GCC'GGC
Cuts at: 0 164 880 2208
Size: 164 716 1328
Fragments arranged by size:
1328 716 164
NarI GG'CG_CC
Cuts at: 0 94 160 257 684 1929 2208
Size: 94 66 97 427 1245 279
Fragments arranged by size:
1245 427 279 97 94 66
NcoI C'CATG_G
Cuts at: 0 1178 1965 2208
Size: 1178 787 243
Fragments arranged by size:
1178 787 243
NgoMIV G'CCGG_C
Cuts at: 0 162 878 2208
Size: 162 716 1330
Fragments arranged by size:
1330 716 162
PasI CC'CwG_GG
Cuts at: 0 1149 2208
Size: 1149 1059
PfoI T'CCnGG_A
Cuts at: 0 440 623 1343 1766 2208
Size: 440 183 720 423 442
Fragments arranged by size:
720 442 440 423 183
PmlI CAC'GTG
Cuts at: 0 2021 2208
Size: 2021 187
PpuMI rG'GwC_Cy
Cuts at: 0 53 310 323 627 867 1866 2208
Size: 53 257 13 304 240 999 342
Fragments arranged by size:
999 342 304 257 240 53 13
PshAI GACnn'nnGTC
Cuts at: 0 597 1736 2208
Size: 597 1139 472
Fragments arranged by size:
1139 597 472
PspOMI G'GGCC_C
Cuts at: 0 284 399 2208
Size: 284 115 1809
Fragments arranged by size:
1809 284 115
PstI C_TGCA'G
Cuts at: 0 482 566 875 1230 1448 2075 2208
Size: 482 84 309 355 218 627 133
Fragments arranged by size:
627 482 355 309 218 133 84
PvuII CAG'CTG
Cuts at: 0 81 520 954 1086 1380 1449 1465 1560
Size: 81 439 434 132 294 69 16 95
Cuts at: 1560 2208
Size: 648
Fragments arranged by size:
648 439 434 294 132 95 81 69
16
SanDI GG'GwC_CC
Cuts at: 0 53 310 2208
Size: 53 257 1898
Fragments arranged by size:
1898 257 53
SapI GCTCTTCn'nnn_
Cuts at: 0 731 1036 2208
Size: 731 305 1172
Fragments arranged by size:
1172 731 305
SexAI A'CCwGG_T
Cuts at: 0 1685 2208
Size: 1685 523
SfcI C'TryA_G
Cuts at: 0 478 562 871 1004 1226 1444 1824 2071
Size: 478 84 309 133 222 218 380 247
Cuts at: 2071 2208
Size: 137
Fragments arranged by size:
478 380 309 247 222 218 137 133
84
SfoI GGC'GCC
Cuts at: 0 95 161 258 685 1930 2208
Size: 95 66 97 427 1245 278
Fragments arranged by size:
1245 427 278 97 95 66
SmaI CCC'GGG
Cuts at: 0 50 89 405 1567 2208
Size: 50 39 316 1162 641
Fragments arranged by size:
1162 641 316 50 39
SmlI C'TyrA_G
Cuts at: 0 1546 2208
Size: 1546 662
SrfI GCCC'GGGC
Cuts at: 0 1567 2208
Size: 1567 641
StuI AGG'CCT
Cuts at: 0 1284 1642 2208
Size: 1284 358 566
Fragments arranged by size:
1284 566 358
StyI C'CwwG_G
Cuts at: 0 1178 1285 1965 2208
Size: 1178 107 680 243
Fragments arranged by size:
1178 680 243 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1696 1757 2208
Size: 1696 61 451
Fragments arranged by size:
1696 451 61
TatI w'GTAC_w
Cuts at: 0 587 2208
Size: 587 1621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2032 2064 2208
Size: 2032 32 144
Fragments arranged by size:
2032 144 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1185 2173 2208
Size: 1185 988 35
Fragments arranged by size:
1185 988 35
XmaI C'CCGG_G
Cuts at: 0 48 87 403 1565 2208
Size: 48 39 316 1162 643
Fragments arranged by size:
1162 643 316 48 39
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbvCI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NcoI NgoMIV PasI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SanDI SapI
SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI
BsiWI BsmI BspEI BspHI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI