Restriction map for
pFN21AE3948
(Linear) (Six-base) MAPSORT of: KIEE3948.seq Check: 8154 from: 1 to: 1077
KIEE3948.orf
With 182 enzymes: SgfI *
March 22, 2012 16:56 ..
AccI GT'mk_AC
Cuts at: 0 603 742 1077
Size: 603 139 335
Fragments arranged by size:
603 335 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 727 1077
Size: 94 633 350
Fragments arranged by size:
633 350 94
AvaI C'yCGr_G
Cuts at: 0 929 1077
Size: 929 148
AvrII C'CTAG_G
Cuts at: 0 546 1077
Size: 546 531
BanII G_rGCy'C
Cuts at: 0 18 1077
Size: 18 1059
BbsI GAAGACnn'nnnn_
Cuts at: 0 907 1077
Size: 907 170
BbvCI CC'TCA_GC
Cuts at: 0 577 1077
Size: 577 500
BglII A'GATC_T
Cuts at: 0 198 1077
Size: 198 879
BlpI GC'TnA_GC
Cuts at: 0 5 557 1077
Size: 5 552 520
Fragments arranged by size:
552 520 5
Bme1580I G_kGCm'C
Cuts at: 0 913 1077
Size: 913 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 122 1077
Size: 122 955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 409 608 652 1077
Size: 193 216 199 44 425
Fragments arranged by size:
425 216 199 193 44
Bpu10I CC'TnA_GC
Cuts at: 0 577 647 1077
Size: 577 70 430
Fragments arranged by size:
577 430 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 1077
Size: 429 648
BsaI GGTCTCn'nnnn_
Cuts at: 0 93 325 1077
Size: 93 232 752
Fragments arranged by size:
752 232 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 669 699 1077
Size: 669 30 378
Fragments arranged by size:
669 378 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1014 1077
Size: 1014 63
BsiHKAI G_wGCw'C
Cuts at: 0 11 638 1077
Size: 11 627 439
Fragments arranged by size:
627 439 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 18 638 913 1077
Size: 11 7 620 275 164
Fragments arranged by size:
620 275 164 11 7
BsrBI CCG'CTC
Cuts at: 0 106 1077
Size: 106 971
BsrGI T'GTAC_A
Cuts at: 0 525 855 1077
Size: 525 330 222
Fragments arranged by size:
525 330 222
BstYI r'GATC_y
Cuts at: 0 198 1077
Size: 198 879
BtsI GCAGTG_nn'
Cuts at: 0 101 571 1077
Size: 101 470 506
Fragments arranged by size:
506 470 101
EaeI y'GGCC_r
Cuts at: 0 38 1077
Size: 38 1039
EarI CTCTTCn'nnn_
Cuts at: 0 113 410 1077
Size: 113 297 667
Fragments arranged by size:
667 297 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 193 409 608 652 727 1077
Size: 94 99 216 199 44 75 350
Fragments arranged by size:
350 216 199 99 94 75 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 856 888 1077
Size: 37 32 787 32 189
Fragments arranged by size:
787 189 37 32 32
HincII GTy'rAC
Cuts at: 0 604 1077
Size: 604 473
HindIII A'AGCT_T
Cuts at: 0 1010 1077
Size: 1010 67
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1077
Size: 1057 20
MspA1I CmG'CkG
Cuts at: 0 28 452 980 1077
Size: 28 424 528 97
Fragments arranged by size:
528 424 97 28
NspI r_CATG'y
Cuts at: 0 118 1077
Size: 118 959
PvuII CAG'CTG
Cuts at: 0 28 1077
Size: 28 1049
SalI G'TCGA_C
Cuts at: 0 602 1077
Size: 602 475
SapI GCTCTTCn'nnn_
Cuts at: 0 113 1077
Size: 113 964
SmaI CCC'GGG
Cuts at: 0 931 1077
Size: 931 146
SmlI C'TyrA_G
Cuts at: 0 408 1077
Size: 408 669
SspI AAT'ATT
Cuts at: 0 427 1077
Size: 427 650
StyI C'CwwG_G
Cuts at: 0 546 1077
Size: 546 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 748 1077
Size: 163 585 329
Fragments arranged by size:
585 329 163
TatI w'GTAC_w
Cuts at: 0 525 855 1041 1077
Size: 525 330 186 36
Fragments arranged by size:
525 330 186 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 669 701 1014 1046 1077
Size: 669 32 313 32 31
Fragments arranged by size:
669 313 32 32 31
XbaI T'CTAG_A
Cuts at: 0 226 1077
Size: 226 851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 1077
Size: 63 1014
XmaI C'CCGG_G
Cuts at: 0 929 1077
Size: 929 148
Enzymes that do cut:
AccI AcuI AvaI AvrII BanII BbsI BbvCI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BsiHKAI Bsp1286I BsrBI BsrGI BstYI BtsI EaeI
EarI Eco57MI Hin4I HincII HindIII MmeI MspA1I NspI
PvuII SalI SapI SmaI SmlI SspI StyI TaqII
TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BmgBI BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XhoI XmnI ZraI