Restriction map for
pFN21AE3946
(Linear) (Six-base) MAPSORT of: KIEE3946.seq Check: 2850 from: 1 to: 873
KIEE3946.orf
With 182 enzymes: SgfI *
March 22, 2012 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 467 873
Size: 467 406
AccI GT'mk_AC
Cuts at: 0 351 873
Size: 351 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 778 873
Size: 331 447 95
Fragments arranged by size:
447 331 95
AfeI AGC'GCT
Cuts at: 0 67 873
Size: 67 806
AleI CACnn'nnGTG
Cuts at: 0 436 873
Size: 436 437
ApaI G_GGCC'C
Cuts at: 0 746 873
Size: 746 127
AvaI C'yCGr_G
Cuts at: 0 50 269 324 873
Size: 50 219 55 549
Fragments arranged by size:
549 219 55 50
BamHI G'GATC_C
Cuts at: 0 243 873
Size: 243 630
BanI G'GyrC_C
Cuts at: 0 328 529 689 824 873
Size: 328 201 160 135 49
Fragments arranged by size:
328 201 160 135 49
BanII G_rGCy'C
Cuts at: 0 446 731 746 873
Size: 446 285 15 127
Fragments arranged by size:
446 285 127 15
BbeI G_GCGC'C
Cuts at: 0 332 873
Size: 332 541
BbsI GAAGACnn'nnnn_
Cuts at: 0 712 849 873
Size: 712 137 24
Fragments arranged by size:
712 137 24
BclI T'GATC_A
Cuts at: 0 257 873
Size: 257 616
BlpI GC'TnA_GC
Cuts at: 0 69 873
Size: 69 804
Bme1580I G_kGCm'C
Cuts at: 0 369 433 532 629 746 784 873
Size: 369 64 99 97 117 38 89
Fragments arranged by size:
369 117 99 97 89 64 38
BmgBI CAC'GTC
Cuts at: 0 810 873
Size: 810 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 272 873
Size: 272 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 206 808 873
Size: 190 16 602 65
Fragments arranged by size:
602 190 65 16
Bpu10I CC'TnA_GC
Cuts at: 0 862 873
Size: 862 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 873
Size: 274 599
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 600 873
Size: 266 334 273
Fragments arranged by size:
334 273 266
BsaAI yAC'GTr
Cuts at: 0 210 873
Size: 210 663
BsaHI Gr'CG_yC
Cuts at: 0 329 395 873
Size: 329 66 478
Fragments arranged by size:
478 329 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 873
Size: 556 30 287
Fragments arranged by size:
556 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 564 873
Size: 70 494 309
Fragments arranged by size:
494 309 70
BseYI C'CCAG_C
Cuts at: 0 332 873
Size: 332 541
BsiHKAI G_wGCw'C
Cuts at: 0 731 868 873
Size: 731 137 5
Fragments arranged by size:
731 137 5
Bsp1286I G_dGCh'C
Cuts at: 0 369 433 446 532 629 731 746 784
Size: 369 64 13 86 97 102 15 38
Cuts at: 784 868 873
Size: 84 5
Fragments arranged by size:
369 102 97 86 84 64 38 15
13 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 467 873
Size: 467 406
BsrBI CCG'CTC
Cuts at: 0 90 873
Size: 90 783
BsrDI GCAATG_nn'
Cuts at: 0 501 873
Size: 501 372
BsrGI T'GTAC_A
Cuts at: 0 764 873
Size: 764 109
BssHII G'CGCG_C
Cuts at: 0 99 101 873
Size: 99 2 772
Fragments arranged by size:
772 99 2
BssSI C'ACGA_G
Cuts at: 0 418 873
Size: 418 455
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 873
Size: 210 663
BstYI r'GATC_y
Cuts at: 0 243 873
Size: 243 630
DraIII CAC_nnn'GTG
Cuts at: 0 491 696 873
Size: 491 205 177
Fragments arranged by size:
491 205 177
EcoICRI GAG'CTC
Cuts at: 0 729 873
Size: 729 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 206 331 778 808 873
Size: 190 16 125 447 30 65
Fragments arranged by size:
447 190 125 65 30 16
EcoO109I rG'GnC_Cy
Cuts at: 0 164 743 873
Size: 164 579 130
Fragments arranged by size:
579 164 130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 873
Size: 873
HaeII r_GCGC'y
Cuts at: 0 69 332 822 873
Size: 69 263 490 51
Fragments arranged by size:
490 263 69 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 873
Size: 664 32 177
Fragments arranged by size:
664 177 32
HincII GTy'rAC
Cuts at: 0 352 873
Size: 352 521
KasI G'GCGC_C
Cuts at: 0 328 873
Size: 328 545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 873
Size: 518 355
MslI CAynn'nnrTG
Cuts at: 0 212 436 873
Size: 212 224 437
Fragments arranged by size:
437 224 212
NarI GG'CG_CC
Cuts at: 0 329 873
Size: 329 544
PmlI CAC'GTG
Cuts at: 0 210 873
Size: 210 663
PpuMI rG'GwC_Cy
Cuts at: 0 164 873
Size: 164 709
PspOMI G'GGCC_C
Cuts at: 0 742 873
Size: 742 131
SacI G_AGCT'C
Cuts at: 0 731 873
Size: 731 142
SalI G'TCGA_C
Cuts at: 0 350 873
Size: 350 523
SanDI GG'GwC_CC
Cuts at: 0 164 873
Size: 164 709
SfoI GGC'GCC
Cuts at: 0 330 873
Size: 330 543
SmaI CCC'GGG
Cuts at: 0 52 326 873
Size: 52 274 547
Fragments arranged by size:
547 274 52
SmlI C'TyrA_G
Cuts at: 0 289 873
Size: 289 584
StyI C'CwwG_G
Cuts at: 0 248 873
Size: 248 625
TatI w'GTAC_w
Cuts at: 0 764 873
Size: 764 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 556 588 873
Size: 556 32 285
Fragments arranged by size:
556 285 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 807 873
Size: 463 344 66
Fragments arranged by size:
463 344 66
XmaI C'CCGG_G
Cuts at: 0 50 324 873
Size: 50 274 549
Fragments arranged by size:
549 274 50
Enzymes that do cut:
AarI AccI AcuI AfeI AleI ApaI AvaI BamHI
BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstXI BstYI DraIII EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MslI NarI
PmlI PpuMI PspOMI SacI SalI SanDI SfoI SmaI
SmlI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BfrBI BglI BglII BmtI
BplI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI