(Linear) (Six-base) MAPSORT of: KIEE3940.seq  Check: 8556  from: 1  to: 444



KIEE3940.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:55  ..

ApaLI G'TGCA_C

Cuts at:      0      97     444 
   Size:         97     347
 
ApoI r'AATT_y

Cuts at:      0     320     444 
   Size:        320     124
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      15     444 
   Size:         15     429
 
Bme1580I G_kGCm'C

Cuts at:      0     101     444 
   Size:        101     343
 
BmgBI CAC'GTC

Cuts at:      0     288     444 
   Size:        288     156
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     232     444 
   Size:        232     212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     444 
   Size:        153     291
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     320     444 
   Size:        320     124
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     444 
   Size:        101     343
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     278     444 
   Size:        278     166
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     444 
   Size:        101     343
 
BstBI TT'CG_AA

Cuts at:      0       6     444 
   Size:          6     438
 
BstYI r'GATC_y

Cuts at:      0      82     444 
   Size:         82     362
 
EaeI y'GGCC_r

Cuts at:      0     271     444 
   Size:        271     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     444 
   Size:        123     321
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     444 
   Size:        123     321
 
PshAI GACnn'nnGTC

Cuts at:      0      48     444 
   Size:         48     396
 
SmlI C'TyrA_G

Cuts at:      0     132     444 
   Size:        132     312
 
SspI AAT'ATT

Cuts at:      0     167     408     444 
   Size:        167     241      36

  Fragments arranged by size: 

                 241     167      36
 
StuI AGG'CCT

Cuts at:      0     278     444 
   Size:        278     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     214     444 
   Size:        214     230
 
Tth111I GACn'n_nGTC

Cuts at:      0     127     444 
   Size:        127     317
 

 Enzymes that do cut:

    ApaLI     ApoI     BbsI Bme1580I    BmgBI     BmrI    BpuEI     BsaI
  BsiHKAI    BsmBI Bsp1286I    BstBI    BstYI     EaeI EcoO109I    PpuMI
    PshAI     SmlI     SspI     StuI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StyI     SwaI     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI