Restriction map for
pFN21AE3938
(Linear) (Six-base) MAPSORT of: KIEE3938.seq Check: 4297 from: 1 to: 573
KIEE3938.orf
With 182 enzymes: SgfI *
March 22, 2012 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 573
Size: 156 417
AccI GT'mk_AC
Cuts at: 0 389 485 573
Size: 389 96 88
Fragments arranged by size:
389 96 88
AleI CACnn'nnGTG
Cuts at: 0 343 573
Size: 343 230
AlwNI CAG_nnn'CTG
Cuts at: 0 72 573
Size: 72 501
AvaI C'yCGr_G
Cuts at: 0 185 573
Size: 185 388
BanII G_rGCy'C
Cuts at: 0 136 194 479 573
Size: 136 58 285 94
Fragments arranged by size:
285 136 94 58
BbvCI CC'TCA_GC
Cuts at: 0 535 573
Size: 535 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 573
Size: 3 34 536
Fragments arranged by size:
536 34 3
BlpI GC'TnA_GC
Cuts at: 0 404 573
Size: 404 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 573
Size: 420 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 493 573
Size: 82 411 80
Fragments arranged by size:
411 82 80
Bpu10I CC'TnA_GC
Cuts at: 0 535 573
Size: 535 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 573
Size: 283 290
BsaAI yAC'GTr
Cuts at: 0 129 573
Size: 129 444
BsaHI Gr'CG_yC
Cuts at: 0 37 573
Size: 37 536
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 448 478 573
Size: 118 30 300 30 95
Fragments arranged by size:
300 118 95 30 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 103 573
Size: 103 470
Bsp1286I G_dGCh'C
Cuts at: 0 136 194 479 573
Size: 136 58 285 94
Fragments arranged by size:
285 136 94 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 573
Size: 156 417
BsrFI r'CCGG_y
Cuts at: 0 458 573
Size: 458 115
BstEII G'GTnAC_C
Cuts at: 0 336 573
Size: 336 237
BtsI GCAGTG_nn'
Cuts at: 0 219 573
Size: 219 354
EaeI y'GGCC_r
Cuts at: 0 30 491 573
Size: 30 461 82
Fragments arranged by size:
461 82 30
EarI CTCTTCn'nnn_
Cuts at: 0 1 573
Size: 1 572
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 573
Size: 469 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 493 573
Size: 82 411 80
Fragments arranged by size:
411 82 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 543 573
Size: 384 159 30
Fragments arranged by size:
384 159 30
HaeII r_GCGC'y
Cuts at: 0 465 573
Size: 465 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 573
Size: 448 32 93
Fragments arranged by size:
448 93 32
MscI TGG'CCA
Cuts at: 0 32 493 573
Size: 32 461 80
Fragments arranged by size:
461 80 32
MslI CAynn'nnrTG
Cuts at: 0 127 343 573
Size: 127 216 230
Fragments arranged by size:
230 216 127
MspA1I CmG'CkG
Cuts at: 0 381 557 573
Size: 381 176 16
Fragments arranged by size:
381 176 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 90 573
Size: 90 483
PfoI T'CCnGG_A
Cuts at: 0 59 470 573
Size: 59 411 103
Fragments arranged by size:
411 103 59
RsrII CG'GwC_CG
Cuts at: 0 456 573
Size: 456 117
SapI GCTCTTCn'nnn_
Cuts at: 0 1 573
Size: 1 572
SfcI C'TryA_G
Cuts at: 0 255 573
Size: 255 318
SmaI CCC'GGG
Cuts at: 0 187 573
Size: 187 386
SmlI C'TyrA_G
Cuts at: 0 298 573
Size: 298 275
StyI C'CwwG_G
Cuts at: 0 320 351 563 573
Size: 320 31 212 10
Fragments arranged by size:
320 212 31 10
XmaI C'CCGG_G
Cuts at: 0 185 573
Size: 185 388
Enzymes that do cut:
AarI AccI AleI AlwNI AvaI BanII BbvCI BcgI
BlpI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BsmBI Bsp1286I BspMI BsrFI BstEII BtsI EaeI EarI
EciI Eco57MI FalI HaeII Hin4I MscI MslI MspA1I
PflMI PfoI RsrII SapI SfcI SmaI SmlI StyI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
BsaI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI