(Linear) (Six-base) MAPSORT of: KIEE3936.seq  Check: 5286  from: 1  to: 537



KIEE3936.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:54  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     406     537 
   Size:        406     131
 
BsiHKAI G_wGCw'C

Cuts at:      0     477     537 
   Size:        477      60
 
Bsp1286I G_dGCh'C

Cuts at:      0     477     537 
   Size:        477      60
 
BspHI T'CATG_A

Cuts at:      0     333     537 
   Size:        333     204
 
BsrDI GCAATG_nn'

Cuts at:      0     156     537 
   Size:        156     381
 
BtgI C'CryG_G

Cuts at:      0     451     537 
   Size:        451      86
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     537 
   Size:         37     500
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     537 
   Size:        160     377
 
MspA1I CmG'CkG

Cuts at:      0     406     537 
   Size:        406     131
 
NcoI C'CATG_G

Cuts at:      0     451     537 
   Size:        451      86
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      23     537 
   Size:         23     514
 
PfoI T'CCnGG_A

Cuts at:      0     226     520     537 
   Size:        226     294      17

  Fragments arranged by size: 

                 294     226      17
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     223     255     537 
   Size:        223      32     282

  Fragments arranged by size: 

                 282     223      32
 
PstI C_TGCA'G

Cuts at:      0     357     537 
   Size:        357     180
 
PvuII CAG'CTG

Cuts at:      0     406     537 
   Size:        406     131
 
SfcI C'TryA_G

Cuts at:      0     353     537 
   Size:        353     184
 
StyI C'CwwG_G

Cuts at:      0     451     498     537 
   Size:        451      47      39

  Fragments arranged by size: 

                 451      47      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     448     480     537 
   Size:        448      32      57

  Fragments arranged by size: 

                 448      57      32
 
XbaI T'CTAG_A

Cuts at:      0     504     537 
   Size:        504      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     122     537 
   Size:        122     415
 

 Enzymes that do cut:

    AlwNI  BsiHKAI Bsp1286I    BspHI    BsrDI     BtgI     EarI     MmeI
   MspA1I     NcoI    PflMI     PfoI     PsrI     PstI    PvuII     SfcI
     StyI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI     ZraI