Restriction map for
pFN21AE3926
(Linear) (Six-base) MAPSORT of: KIEE3926.seq Check: 2765 from: 1 to: 1653
KIEE3926.orf
With 182 enzymes: SgfI *
March 22, 2012 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 374 468 1653
Size: 374 94 1185
Fragments arranged by size:
1185 374 94
AatII G_ACGT'C
Cuts at: 0 1174 1653
Size: 1174 479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 703 1038 1653
Size: 226 477 335 615
Fragments arranged by size:
615 477 335 226
AleI CACnn'nnGTG
Cuts at: 0 1063 1653
Size: 1063 590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1081 1653
Size: 1081 572
AlwNI CAG_nnn'CTG
Cuts at: 0 726 807 1245 1568 1653
Size: 726 81 438 323 85
Fragments arranged by size:
726 438 323 85 81
ApaI G_GGCC'C
Cuts at: 0 1183 1253 1653
Size: 1183 70 400
Fragments arranged by size:
1183 400 70
ApaLI G'TGCA_C
Cuts at: 0 1303 1653
Size: 1303 350
AvaI C'yCGr_G
Cuts at: 0 640 814 1653
Size: 640 174 839
Fragments arranged by size:
839 640 174
BanI G'GyrC_C
Cuts at: 0 333 392 1065 1291 1653
Size: 333 59 673 226 362
Fragments arranged by size:
673 362 333 226 59
BanII G_rGCy'C
Cuts at: 0 38 853 1183 1253 1653
Size: 38 815 330 70 400
Fragments arranged by size:
815 400 330 70 38
BbeI G_GCGC'C
Cuts at: 0 337 1295 1653
Size: 337 958 358
Fragments arranged by size:
958 358 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1377 1411 1653
Size: 1377 34 242
Fragments arranged by size:
1377 242 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1346 1653
Size: 1346 307
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1187 1248 1653
Size: 290 897 61 405
Fragments arranged by size:
897 405 290 61
Bme1580I G_kGCm'C
Cuts at: 0 395 1070 1183 1213 1253 1307 1493 1653
Size: 395 675 113 30 40 54 186 160
Fragments arranged by size:
675 395 186 160 113 54 40 30
BmgBI CAC'GTC
Cuts at: 0 417 543 1653
Size: 417 126 1110
Fragments arranged by size:
1110 417 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 1368 1533 1653
Size: 1368 165 120
Fragments arranged by size:
1368 165 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 447 619 1653
Size: 214 233 172 1034
Fragments arranged by size:
1034 233 214 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1293 1653
Size: 1293 360
BsaAI yAC'GTr
Cuts at: 0 246 354 1644 1653
Size: 246 108 1290 9
Fragments arranged by size:
1290 246 108 9
BsaHI Gr'CG_yC
Cuts at: 0 334 1171 1292 1577 1653
Size: 334 837 121 285 76
Fragments arranged by size:
837 334 285 121 76
BsaWI w'CCGG_w
Cuts at: 0 566 1653
Size: 566 1087
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 970 1000 1450 1480 1499 1529 1653
Size: 970 30 450 30 19 30 124
Fragments arranged by size:
970 450 124 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 46 616 679 1653
Size: 43 3 570 63 974
Fragments arranged by size:
974 570 63 43 3
BseYI C'CCAG_C
Cuts at: 0 846 1355 1549 1583 1653
Size: 846 509 194 34 70
Fragments arranged by size:
846 509 194 70 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 97 226 253 376 700 793 927
Size: 40 57 129 27 123 324 93 134
Cuts at: 927 1653
Size: 726
Fragments arranged by size:
726 324 134 129 123 93 57 40
27
BsiEI CG_ry'CG
Cuts at: 0 1386 1653
Size: 1386 267
BsiHKAI G_wGCw'C
Cuts at: 0 38 1307 1653
Size: 38 1269 346
Fragments arranged by size:
1269 346 38
BsiWI C'GTAC_G
Cuts at: 0 147 1653
Size: 147 1506
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1162 1653
Size: 1162 491
Bsp1286I G_dGCh'C
Cuts at: 0 38 395 853 1070 1183 1213 1253 1307
Size: 38 357 458 217 113 30 40 54
Cuts at: 1307 1493 1653
Size: 186 160
Fragments arranged by size:
458 357 217 186 160 113 54 40
38 30
BspEI T'CCGG_A
Cuts at: 0 566 1653
Size: 566 1087
BspHI T'CATG_A
Cuts at: 0 578 893 1653
Size: 578 315 760
Fragments arranged by size:
760 578 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 468 746 949 1653
Size: 374 94 278 203 704
Fragments arranged by size:
704 374 278 203 94
BsrBI CCG'CTC
Cuts at: 0 1466 1515 1653
Size: 1466 49 138
Fragments arranged by size:
1466 138 49
BsrFI r'CCGG_y
Cuts at: 0 258 593 1062 1653
Size: 258 335 469 591
Fragments arranged by size:
591 469 335 258
BsrGI T'GTAC_A
Cuts at: 0 632 1653
Size: 632 1021
BssHII G'CGCG_C
Cuts at: 0 515 1653
Size: 515 1138
BssSI C'ACGA_G
Cuts at: 0 945 1653
Size: 945 708
BstAPI GCAn_nnn'nTGC
Cuts at: 0 723 807 1365 1653
Size: 723 84 558 288
Fragments arranged by size:
723 558 288 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 1653
Size: 222 1431
BstYI r'GATC_y
Cuts at: 0 548 1653
Size: 548 1105
BtgI C'CryG_G
Cuts at: 0 288 387 1430 1440 1653
Size: 288 99 1043 10 213
Fragments arranged by size:
1043 288 213 99 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1313 1653
Size: 1313 340
BtsI GCAGTG_nn'
Cuts at: 0 270 462 1653
Size: 270 192 1191
Fragments arranged by size:
1191 270 192
DraIII CAC_nnn'GTG
Cuts at: 0 276 534 1275 1644 1653
Size: 276 258 741 369 9
Fragments arranged by size:
741 369 276 258 9
EaeI y'GGCC_r
Cuts at: 0 482 975 1010 1191 1383 1433 1446 1470
Size: 482 493 35 181 192 50 13 24
Cuts at: 1470 1593 1653
Size: 123 60
Fragments arranged by size:
493 482 192 181 123 60 50 35
24 13
EagI C'GGCC_G
Cuts at: 0 1383 1653
Size: 1383 270
EarI CTCTTCn'nnn_
Cuts at: 0 1000 1653
Size: 1000 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 424 1135 1338 1653
Size: 19 405 711 203 315
Fragments arranged by size:
711 405 315 203 19
EcoICRI GAG'CTC
Cuts at: 0 36 1653
Size: 36 1617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 226 447 619 703 1038 1653
Size: 214 12 221 172 84 335 615
Fragments arranged by size:
615 335 221 214 172 84 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 403 661 763 1653
Size: 403 258 102 890
Fragments arranged by size:
890 403 258 102
EcoO109I rG'GnC_Cy
Cuts at: 0 735 756 932 1048 1180 1249 1250 1653
Size: 735 21 176 116 132 69 1 403
Fragments arranged by size:
735 403 176 132 116 69 21 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 1653
Size: 39 1614
HaeII r_GCGC'y
Cuts at: 0 268 337 657 808 1295 1653
Size: 268 69 320 151 487 358
Fragments arranged by size:
487 358 320 268 151 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1216 1248 1653
Size: 1159 32 25 32 405
Fragments arranged by size:
1159 405 32 32 25
KasI G'GCGC_C
Cuts at: 0 333 1291 1653
Size: 333 958 362
Fragments arranged by size:
958 362 333
MscI TGG'CCA
Cuts at: 0 1012 1435 1653
Size: 1012 423 218
Fragments arranged by size:
1012 423 218
MslI CAynn'nnrTG
Cuts at: 0 1063 1653
Size: 1063 590
MspA1I CmG'CkG
Cuts at: 0 81 140 290 300 389 512 630 723
Size: 81 59 150 10 89 123 118 93
Cuts at: 723 861 1029 1220 1653
Size: 138 168 191 433
Fragments arranged by size:
433 191 168 150 138 123 118 93
89 81 59 10
NaeI GCC'GGC
Cuts at: 0 260 1653
Size: 260 1393
NarI GG'CG_CC
Cuts at: 0 334 1292 1653
Size: 334 958 361
Fragments arranged by size:
958 361 334
NcoI C'CATG_G
Cuts at: 0 1440 1653
Size: 1440 213
NgoMIV G'CCGG_C
Cuts at: 0 258 1653
Size: 258 1395
NotI GC'GGCC_GC
Cuts at: 0 1383 1653
Size: 1383 270
NspI r_CATG'y
Cuts at: 0 828 1653
Size: 828 825
PasI CC'CwG_GG
Cuts at: 0 1245 1653
Size: 1245 408
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 1653
Size: 726 927
PmlI CAC'GTG
Cuts at: 0 246 354 1653
Size: 246 108 1299
Fragments arranged by size:
1299 246 108
PpuMI rG'GwC_Cy
Cuts at: 0 735 756 932 1653
Size: 735 21 176 721
Fragments arranged by size:
735 721 176 21
PspOMI G'GGCC_C
Cuts at: 0 1179 1249 1653
Size: 1179 70 404
Fragments arranged by size:
1179 404 70
PspXI vC'TCGA_Gb
Cuts at: 0 640 1653
Size: 640 1013
PstI C_TGCA'G
Cuts at: 0 371 704 787 860 1079 1258 1653
Size: 371 333 83 73 219 179 395
Fragments arranged by size:
395 371 333 219 179 83 73
PvuII CAG'CTG
Cuts at: 0 81 630 723 861 1029 1653
Size: 81 549 93 138 168 624
Fragments arranged by size:
624 549 168 138 93 81
SacI G_AGCT'C
Cuts at: 0 38 1653
Size: 38 1615
SacII CC_GC'GG
Cuts at: 0 291 390 1653
Size: 291 99 1263
Fragments arranged by size:
1263 291 99
SapI GCTCTTCn'nnn_
Cuts at: 0 1000 1653
Size: 1000 653
ScaI AGT'ACT
Cuts at: 0 496 1504 1653
Size: 496 1008 149
Fragments arranged by size:
1008 496 149
SfcI C'TryA_G
Cuts at: 0 367 507 700 783 856 1075 1254 1608
Size: 367 140 193 83 73 219 179 354
Cuts at: 1608 1653
Size: 45
Fragments arranged by size:
367 354 219 193 179 140 83 73
45
SfoI GGC'GCC
Cuts at: 0 335 1293 1653
Size: 335 958 360
Fragments arranged by size:
958 360 335
SgrAI Cr'CCGG_yG
Cuts at: 0 258 1062 1653
Size: 258 804 591
Fragments arranged by size:
804 591 258
SmlI C'TyrA_G
Cuts at: 0 640 1308 1653
Size: 640 668 345
Fragments arranged by size:
668 640 345
SphI G_CATG'C
Cuts at: 0 828 1653
Size: 828 825
StuI AGG'CCT
Cuts at: 0 172 1653
Size: 172 1481
StyI C'CwwG_G
Cuts at: 0 131 1440 1653
Size: 131 1309 213
Fragments arranged by size:
1309 213 131
TatI w'GTAC_w
Cuts at: 0 494 632 1502 1653
Size: 494 138 870 151
Fragments arranged by size:
870 494 151 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 518 550 970 1002 1499 1517 1531 1549
Size: 518 32 420 32 497 18 14 18
Cuts at: 1549 1653
Size: 104
Fragments arranged by size:
518 497 420 104 32 32 18 18
14
Tth111I GACn'n_nGTC
Cuts at: 0 1173 1653
Size: 1173 480
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 843 1269 1653
Size: 843 426 384
Fragments arranged by size:
843 426 384
XhoI C'TCGA_G
Cuts at: 0 640 1653
Size: 640 1013
XmnI GAAnn'nnTTC
Cuts at: 0 22 1653
Size: 22 1631
ZraI GAC'GTC
Cuts at: 0 1172 1653
Size: 1172 481
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BcgI BciVI BglI Bme1580I
BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PflMI PmlI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SapI ScaI
SfcI SfoI SgrAI SmlI SphI StuI StyI TatI
TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BclI BfrBI BglII BlpI BmtI BplI
Bpu10I BsaI BsaBI BsmI BsrDI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI
SanDI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII XbaI XmaI