Restriction map for
pFN21AE3925
(Linear) (Six-base) MAPSORT of: KIEE3925.seq Check: 4502 from: 1 to: 669
KIEE3925.orf
With 182 enzymes: SgfI *
March 22, 2012 16:52 ..
Acc65I G'GTAC_C
Cuts at: 0 172 669
Size: 172 497
AlwNI CAG_nnn'CTG
Cuts at: 0 28 72 371 449 507 669
Size: 28 44 299 78 58 162
Fragments arranged by size:
299 162 78 58 44 28
ApaI G_GGCC'C
Cuts at: 0 205 228 435 669
Size: 205 23 207 234
Fragments arranged by size:
234 207 205 23
BanI G'GyrC_C
Cuts at: 0 172 669
Size: 172 497
BanII G_rGCy'C
Cuts at: 0 93 205 228 435 669
Size: 93 112 23 207 234
Fragments arranged by size:
234 207 112 93 23
BbvCI CC'TCA_GC
Cuts at: 0 112 304 669
Size: 112 192 365
Fragments arranged by size:
365 192 112
Bme1580I G_kGCm'C
Cuts at: 0 205 228 435 565 669
Size: 205 23 207 130 104
Fragments arranged by size:
207 205 130 104 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 274 283 403 669
Size: 175 99 9 120 266
Fragments arranged by size:
266 175 120 99 9
Bpu10I CC'TnA_GC
Cuts at: 0 112 304 319 669
Size: 112 192 15 350
Fragments arranged by size:
350 192 112 15
BsaAI yAC'GTr
Cuts at: 0 560 669
Size: 560 109
BsaHI Gr'CG_yC
Cuts at: 0 182 669
Size: 182 487
BseYI C'CCAG_C
Cuts at: 0 98 428 602 669
Size: 98 330 174 67
Fragments arranged by size:
330 174 98 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 669
Size: 549 120
BsiHKAI G_wGCw'C
Cuts at: 0 290 389 669
Size: 290 99 280
Fragments arranged by size:
290 280 99
Bsp1286I G_dGCh'C
Cuts at: 0 93 205 228 290 389 435 565 669
Size: 93 112 23 62 99 46 130 104
Fragments arranged by size:
130 112 104 99 93 62 46 23
BsrGI T'GTAC_A
Cuts at: 0 2 669
Size: 2 667
BstAPI GCAn_nnn'nTGC
Cuts at: 0 28 669
Size: 28 641
Bsu36I CC'TnA_GG
Cuts at: 0 168 669
Size: 168 501
BtsI GCAGTG_nn'
Cuts at: 0 123 402 669
Size: 123 279 267
Fragments arranged by size:
279 267 123
EaeI y'GGCC_r
Cuts at: 0 45 362 451 554 578 669
Size: 45 317 89 103 24 91
Fragments arranged by size:
317 103 91 89 45 24
EarI CTCTTCn'nnn_
Cuts at: 0 135 669
Size: 135 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 274 283 403 669
Size: 175 99 9 120 266
Fragments arranged by size:
266 175 120 99 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 453 669
Size: 453 216
EcoO109I rG'GnC_Cy
Cuts at: 0 69 201 202 225 669
Size: 69 132 1 23 444
Fragments arranged by size:
444 132 69 23 1
HaeII r_GCGC'y
Cuts at: 0 397 669
Size: 397 272
KpnI G_GTAC'C
Cuts at: 0 176 669
Size: 176 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 527 669
Size: 218 309 142
Fragments arranged by size:
309 218 142
MscI TGG'CCA
Cuts at: 0 364 453 556 669
Size: 364 89 103 113
Fragments arranged by size:
364 113 103 89
MslI CAynn'nnrTG
Cuts at: 0 574 669
Size: 574 95
MspA1I CmG'CkG
Cuts at: 0 102 303 312 482 606 669
Size: 102 201 9 170 124 63
Fragments arranged by size:
201 170 124 102 63 9
PasI CC'CwG_GG
Cuts at: 0 72 669
Size: 72 597
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 449 669
Size: 72 377 220
Fragments arranged by size:
377 220 72
PmlI CAC'GTG
Cuts at: 0 560 669
Size: 560 109
PshAI GACnn'nnGTC
Cuts at: 0 185 669
Size: 185 484
PspOMI G'GGCC_C
Cuts at: 0 201 224 431 669
Size: 201 23 207 238
Fragments arranged by size:
238 207 201 23
PstI C_TGCA'G
Cuts at: 0 24 33 233 256 409 464 487 669
Size: 24 9 200 23 153 55 23 182
Fragments arranged by size:
200 182 153 55 24 23 23 9
PvuII CAG'CTG
Cuts at: 0 102 303 312 482 606 669
Size: 102 201 9 170 124 63
Fragments arranged by size:
201 170 124 102 63 9
SapI GCTCTTCn'nnn_
Cuts at: 0 135 669
Size: 135 534
SbfI CC_TGCA'GG
Cuts at: 0 24 233 669
Size: 24 209 436
Fragments arranged by size:
436 209 24
SfcI C'TryA_G
Cuts at: 0 20 29 229 252 405 460 483 669
Size: 20 9 200 23 153 55 23 186
Fragments arranged by size:
200 186 153 55 23 23 20 9
StuI AGG'CCT
Cuts at: 0 166 669
Size: 166 503
StyI C'CwwG_G
Cuts at: 0 176 281 454 669
Size: 176 105 173 215
Fragments arranged by size:
215 176 173 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2 669
Size: 2 667
TatI w'GTAC_w
Cuts at: 0 2 669
Size: 2 667
Enzymes that do cut:
Acc65I AlwNI ApaI BanI BanII BbvCI Bme1580I BpmI
Bpu10I BsaAI BsaHI BseYI BsgI BsiHKAI Bsp1286I BsrGI
BstAPI Bsu36I BtsI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII KpnI MmeI MscI MslI MspA1I PasI PflMI
PmlI PshAI PspOMI PstI PvuII SapI SbfI SfcI
StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI