Restriction map for
pFN21AE3924
(Linear) (Six-base) MAPSORT of: KIEE3924.seq Check: 4058 from: 1 to: 1038
KIEE3924.orf
With 182 enzymes: SgfI *
March 22, 2012 16:51 ..
AflIII A'CryG_T
Cuts at: 0 252 531 1038
Size: 252 279 507
Fragments arranged by size:
507 279 252
AlwNI CAG_nnn'CTG
Cuts at: 0 204 348 606 1038
Size: 204 144 258 432
Fragments arranged by size:
432 258 204 144
ApaI G_GGCC'C
Cuts at: 0 699 1038
Size: 699 339
ApaLI G'TGCA_C
Cuts at: 0 876 1038
Size: 876 162
BanI G'GyrC_C
Cuts at: 0 91 642 902 1038
Size: 91 551 260 136
Fragments arranged by size:
551 260 136 91
BanII G_rGCy'C
Cuts at: 0 699 1038
Size: 699 339
BbeI G_GCGC'C
Cuts at: 0 95 646 1038
Size: 95 551 392
Fragments arranged by size:
551 392 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 552 1038
Size: 483 69 486
Fragments arranged by size:
486 483 69
BbvCI CC'TCA_GC
Cuts at: 0 607 1038
Size: 607 431
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 1038
Size: 242 796
BglI GCCn_nnn'nGGC
Cuts at: 0 402 1038
Size: 402 636
Bme1580I G_kGCm'C
Cuts at: 0 260 699 880 1038
Size: 260 439 181 158
Fragments arranged by size:
439 260 181 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 1038
Size: 171 867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 358 505 910 1038
Size: 148 210 147 405 128
Fragments arranged by size:
405 210 148 147 128
Bpu10I CC'TnA_GC
Cuts at: 0 414 607 1038
Size: 414 193 431
Fragments arranged by size:
431 414 193
BsaAI yAC'GTr
Cuts at: 0 596 1038
Size: 596 442
BsaHI Gr'CG_yC
Cuts at: 0 92 643 1038
Size: 92 551 395
Fragments arranged by size:
551 395 92
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 252 483 862 907 1038
Size: 226 26 231 379 45 131
Fragments arranged by size:
379 231 226 131 45 26
BseYI C'CCAG_C
Cuts at: 0 717 938 1038
Size: 717 221 100
Fragments arranged by size:
717 221 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 721 796 981 1038
Size: 82 639 75 185 57
Fragments arranged by size:
639 185 82 75 57
BsiEI CG_ry'CG
Cuts at: 0 11 1038
Size: 11 1027
BsiHKAI G_wGCw'C
Cuts at: 0 880 1038
Size: 880 158
Bsp1286I G_dGCh'C
Cuts at: 0 260 699 880 1038
Size: 260 439 181 158
Fragments arranged by size:
439 260 181 158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 1038
Size: 320 718
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 744 884 1038
Size: 348 396 140 154
Fragments arranged by size:
396 348 154 140
BstYI r'GATC_y
Cuts at: 0 615 1038
Size: 615 423
Bsu36I CC'TnA_GG
Cuts at: 0 622 691 1038
Size: 622 69 347
Fragments arranged by size:
622 347 69
BtgI C'CryG_G
Cuts at: 0 63 273 552 752 816 1038
Size: 63 210 279 200 64 222
Fragments arranged by size:
279 222 210 200 64 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 227 1038
Size: 227 811
BtsI GCAGTG_nn'
Cuts at: 0 730 1038
Size: 730 308
DraIII CAC_nnn'GTG
Cuts at: 0 744 884 956 1038
Size: 744 140 72 82
Fragments arranged by size:
744 140 82 72
EaeI y'GGCC_r
Cuts at: 0 8 446 572 924 1038
Size: 8 438 126 352 114
Fragments arranged by size:
438 352 126 114 8
EagI C'GGCC_G
Cuts at: 0 8 1038
Size: 8 1030
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 988 1038
Size: 988 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 358 505 910 1038
Size: 148 210 147 405 128
Fragments arranged by size:
405 210 148 147 128
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 340 502 1038
Size: 208 132 162 536
Fragments arranged by size:
536 208 162 132
EcoO109I rG'GnC_Cy
Cuts at: 0 324 507 627 687 695 836 1038
Size: 324 183 120 60 8 141 202
Fragments arranged by size:
324 202 183 141 120 60 8
HaeII r_GCGC'y
Cuts at: 0 95 429 478 607 646 1038
Size: 95 334 49 129 39 392
Fragments arranged by size:
392 334 129 95 49 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 600 632 1038
Size: 494 32 74 32 406
Fragments arranged by size:
494 406 74 32 32
KasI G'GCGC_C
Cuts at: 0 91 642 1038
Size: 91 551 396
Fragments arranged by size:
551 396 91
MscI TGG'CCA
Cuts at: 0 574 1038
Size: 574 464
MslI CAynn'nnrTG
Cuts at: 0 232 1038
Size: 232 806
MspA1I CmG'CkG
Cuts at: 0 65 525 765 783 818 1038
Size: 65 460 240 18 35 220
Fragments arranged by size:
460 240 220 65 35 18
NarI GG'CG_CC
Cuts at: 0 92 643 1038
Size: 92 551 395
Fragments arranged by size:
551 395 92
NcoI C'CATG_G
Cuts at: 0 752 1038
Size: 752 286
NspI r_CATG'y
Cuts at: 0 256 298 535 1038
Size: 256 42 237 503
Fragments arranged by size:
503 256 237 42
PasI CC'CwG_GG
Cuts at: 0 204 1038
Size: 204 834
PciI A'CATG_T
Cuts at: 0 252 531 1038
Size: 252 279 507
Fragments arranged by size:
507 279 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 1038
Size: 204 834
PpuMI rG'GwC_Cy
Cuts at: 0 627 687 1038
Size: 627 60 351
Fragments arranged by size:
627 351 60
PspOMI G'GGCC_C
Cuts at: 0 695 1038
Size: 695 343
PstI C_TGCA'G
Cuts at: 0 353 764 1038
Size: 353 411 274
Fragments arranged by size:
411 353 274
PvuII CAG'CTG
Cuts at: 0 765 1038
Size: 765 273
SacII CC_GC'GG
Cuts at: 0 66 819 1038
Size: 66 753 219
Fragments arranged by size:
753 219 66
ScaI AGT'ACT
Cuts at: 0 58 1038
Size: 58 980
SexAI A'CCwGG_T
Cuts at: 0 740 1038
Size: 740 298
SfcI C'TryA_G
Cuts at: 0 349 708 760 1038
Size: 349 359 52 278
Fragments arranged by size:
359 349 278 52
SfoI GGC'GCC
Cuts at: 0 93 644 1038
Size: 93 551 394
Fragments arranged by size:
551 394 93
SphI G_CATG'C
Cuts at: 0 298 1038
Size: 298 740
StyI C'CwwG_G
Cuts at: 0 752 842 1038
Size: 752 90 196
Fragments arranged by size:
752 196 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 920 1038
Size: 920 118
TatI w'GTAC_w
Cuts at: 0 56 1038
Size: 56 982
Enzymes that do cut:
AflIII AlwNI ApaI ApaLI BanI BanII BbeI BbsI
BbvCI BciVI BglI Bme1580I BplI BpmI Bpu10I BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MscI MslI MspA1I NarI NcoI NspI PasI PciI
PflMI PpuMI PspOMI PstI PvuII SacII ScaI SexAI
SfcI SfoI SphI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI