Restriction map for
pFN21AE3923
(Linear) (Six-base) MAPSORT of: KIEE3923.seq Check: 9947 from: 1 to: 1341
KIEE3923.orf
With 182 enzymes: SgfI *
March 22, 2012 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1158 1341
Size: 1158 183
AccI GT'mk_AC
Cuts at: 0 417 516 1071 1341
Size: 417 99 555 270
Fragments arranged by size:
555 417 270 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1341
Size: 679 662
AfeI AGC'GCT
Cuts at: 0 340 1341
Size: 340 1001
AflIII A'CryG_T
Cuts at: 0 363 768 1341
Size: 363 405 573
Fragments arranged by size:
573 405 363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 1341
Size: 358 983
ApoI r'AATT_y
Cuts at: 0 501 681 922 1081 1326 1341
Size: 501 180 241 159 245 15
Fragments arranged by size:
501 245 241 180 159 15
AvrII C'CTAG_G
Cuts at: 0 307 1341
Size: 307 1034
BanI G'GyrC_C
Cuts at: 0 8 83 1341
Size: 8 75 1258
Fragments arranged by size:
1258 75 8
BanII G_rGCy'C
Cuts at: 0 307 1341
Size: 307 1034
BbeI G_GCGC'C
Cuts at: 0 12 87 1341
Size: 12 75 1254
Fragments arranged by size:
1254 75 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 213 434 1341
Size: 213 221 907
Fragments arranged by size:
907 221 213
BclI T'GATC_A
Cuts at: 0 1047 1341
Size: 1047 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1341
Size: 514 827
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 1341
Size: 403 938
BsaHI Gr'CG_yC
Cuts at: 0 9 84 1341
Size: 9 75 1257
Fragments arranged by size:
1257 75 9
BsaWI w'CCGG_w
Cuts at: 0 172 1341
Size: 172 1169
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 1261 1341
Size: 511 750 80
Fragments arranged by size:
750 511 80
BseYI C'CCAG_C
Cuts at: 0 61 1341
Size: 61 1280
BsiHKAI G_wGCw'C
Cuts at: 0 835 1341
Size: 835 506
BsmI GAATG_Cn'
Cuts at: 0 627 871 1341
Size: 627 244 470
Fragments arranged by size:
627 470 244
Bsp1286I G_dGCh'C
Cuts at: 0 307 835 1341
Size: 307 528 506
Fragments arranged by size:
528 506 307
BspEI T'CCGG_A
Cuts at: 0 172 1341
Size: 172 1169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1158 1192 1341
Size: 1158 34 149
Fragments arranged by size:
1158 149 34
BsrFI r'CCGG_y
Cuts at: 0 5 1341
Size: 5 1336
BsrGI T'GTAC_A
Cuts at: 0 881 1341
Size: 881 460
BstBI TT'CG_AA
Cuts at: 0 753 1341
Size: 753 588
BstEII G'GTnAC_C
Cuts at: 0 290 1341
Size: 290 1051
BstYI r'GATC_y
Cuts at: 0 848 1341
Size: 848 493
BtsI GCAGTG_nn'
Cuts at: 0 74 1341
Size: 74 1267
EaeI y'GGCC_r
Cuts at: 0 31 1341
Size: 31 1310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 679 1341
Size: 514 165 662
Fragments arranged by size:
662 514 165
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 1341
Size: 493 848
EcoO109I rG'GnC_Cy
Cuts at: 0 1294 1341
Size: 1294 47
EcoRI G'AATT_C
Cuts at: 0 1081 1341
Size: 1081 260
HaeII r_GCGC'y
Cuts at: 0 12 56 87 342 1341
Size: 12 44 31 255 999
Fragments arranged by size:
999 255 44 31 12
HincII GTy'rAC
Cuts at: 0 1072 1341
Size: 1072 269
KasI G'GCGC_C
Cuts at: 0 8 83 1341
Size: 8 75 1258
Fragments arranged by size:
1258 75 8
MspA1I CmG'CkG
Cuts at: 0 20 41 1341
Size: 20 21 1300
Fragments arranged by size:
1300 21 20
NaeI GCC'GGC
Cuts at: 0 7 1341
Size: 7 1334
NarI GG'CG_CC
Cuts at: 0 9 84 1341
Size: 9 75 1257
Fragments arranged by size:
1257 75 9
NgoMIV G'CCGG_C
Cuts at: 0 5 1341
Size: 5 1336
NspI r_CATG'y
Cuts at: 0 367 772 1341
Size: 367 405 569
Fragments arranged by size:
569 405 367
PciI A'CATG_T
Cuts at: 0 363 768 1341
Size: 363 405 573
Fragments arranged by size:
573 405 363
PfoI T'CCnGG_A
Cuts at: 0 491 1341
Size: 491 850
PpuMI rG'GwC_Cy
Cuts at: 0 1294 1341
Size: 1294 47
PstI C_TGCA'G
Cuts at: 0 19 52 695 1341
Size: 19 33 643 646
Fragments arranged by size:
646 643 33 19
PvuII CAG'CTG
Cuts at: 0 20 1341
Size: 20 1321
SalI G'TCGA_C
Cuts at: 0 1070 1341
Size: 1070 271
SfcI C'TryA_G
Cuts at: 0 15 48 691 1341
Size: 15 33 643 650
Fragments arranged by size:
650 643 33 15
SfoI GGC'GCC
Cuts at: 0 10 85 1341
Size: 10 75 1256
Fragments arranged by size:
1256 75 10
SpeI A'CTAG_T
Cuts at: 0 210 1341
Size: 210 1131
StyI C'CwwG_G
Cuts at: 0 307 1341
Size: 307 1034
TatI w'GTAC_w
Cuts at: 0 385 881 1341
Size: 385 496 460
Fragments arranged by size:
496 460 385
XmnI GAAnn'nnTTC
Cuts at: 0 749 1341
Size: 749 592
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI ApoI AvrII
BanI BanII BbeI BbsI BclI BpmI BsaI BsaHI
BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrFI BsrGI BstBI BstEII BstYI BtsI EaeI Eco57MI
EcoNI EcoO109I EcoRI HaeII HincII KasI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PpuMI PstI PvuII
SalI SfcI SfoI SpeI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaXI BsgI BsiEI BsiWI BsmBI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI ZraI