Restriction map for
pFN21AE3921
(Linear) (Six-base) MAPSORT of: KIEE3921.seq Check: 2952 from: 1 to: 1197
KIEE3921.orf
With 182 enzymes: SgfI *
March 22, 2012 16:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1197
Size: 384 813
AfeI AGC'GCT
Cuts at: 0 1163 1197
Size: 1163 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1029 1197
Size: 1029 168
AlwNI CAG_nnn'CTG
Cuts at: 0 616 1127 1197
Size: 616 511 70
Fragments arranged by size:
616 511 70
ApoI r'AATT_y
Cuts at: 0 330 513 756 997 1197
Size: 330 183 243 241 200
Fragments arranged by size:
330 243 241 200 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 174 208 1197
Size: 174 34 989
Fragments arranged by size:
989 174 34
BclI T'GATC_A
Cuts at: 0 555 1197
Size: 555 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1197
Size: 1134 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 259 1197
Size: 259 938
BsaHI Gr'CG_yC
Cuts at: 0 28 1197
Size: 28 1169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1116 1146 1197
Size: 1116 30 51
Fragments arranged by size:
1116 51 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 106 109 118 1197
Size: 37 69 3 9 1079
Fragments arranged by size:
1079 69 37 9 3
BseYI C'CCAG_C
Cuts at: 0 616 1197
Size: 616 581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1197
Size: 718 479
BsiEI CG_ry'CG
Cuts at: 0 56 1197
Size: 56 1141
BsmI GAATG_Cn'
Cuts at: 0 1016 1197
Size: 1016 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 19 1197
Size: 19 1178
BspHI T'CATG_A
Cuts at: 0 1089 1197
Size: 1089 108
BsrDI GCAATG_nn'
Cuts at: 0 806 1197
Size: 806 391
BsrFI r'CCGG_y
Cuts at: 0 37 1197
Size: 37 1160
BstEII G'GTnAC_C
Cuts at: 0 1132 1197
Size: 1132 65
ClaI AT'CG_AT
Cuts at: 0 866 1197
Size: 866 331
DraI TTT'AAA
Cuts at: 0 983 1197
Size: 983 214
EaeI y'GGCC_r
Cuts at: 0 53 1197
Size: 53 1144
EagI C'GGCC_G
Cuts at: 0 53 1197
Size: 53 1144
EarI CTCTTCn'nnn_
Cuts at: 0 95 1197
Size: 95 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 137 1197
Size: 34 103 1060
Fragments arranged by size:
1060 103 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1134 1197
Size: 384 750 63
Fragments arranged by size:
750 384 63
HaeII r_GCGC'y
Cuts at: 0 170 1165 1197
Size: 170 995 32
Fragments arranged by size:
995 170 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 823 855 922 954 1116 1148
Size: 532 32 259 32 67 32 162 32
Cuts at: 1148 1197
Size: 49
Fragments arranged by size:
532 259 162 67 49 32 32 32
32
HincII GTy'rAC
Cuts at: 0 501 1197
Size: 501 696
HindIII A'AGCT_T
Cuts at: 0 773 1032 1197
Size: 773 259 165
Fragments arranged by size:
773 259 165
HpaI GTT'AAC
Cuts at: 0 501 1197
Size: 501 696
MspA1I CmG'CkG
Cuts at: 0 215 1197
Size: 215 982
NaeI GCC'GGC
Cuts at: 0 39 1197
Size: 39 1158
NgoMIV G'CCGG_C
Cuts at: 0 37 1197
Size: 37 1160
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 1197
Size: 237 960
PsiI TTA'TAA
Cuts at: 0 299 1197
Size: 299 898
PstI C_TGCA'G
Cuts at: 0 11 1197
Size: 11 1186
SfcI C'TryA_G
Cuts at: 0 7 1197
Size: 7 1190
SpeI A'CTAG_T
Cuts at: 0 652 1141 1197
Size: 652 489 56
Fragments arranged by size:
652 489 56
SspI AAT'ATT
Cuts at: 0 674 1197
Size: 674 523
StyI C'CwwG_G
Cuts at: 0 725 1197
Size: 725 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 1096 1197
Size: 273 823 101
Fragments arranged by size:
823 273 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 1197
Size: 234 963
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApoI BcgI BclI BpmI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI
BsmBI BspHI BsrDI BsrFI BstEII ClaI DraI EaeI
EagI EarI EciI Eco57MI HaeII Hin4I HincII HindIII
HpaI MspA1I NaeI NgoMIV PflMI PsiI PstI SfcI
SpeI SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI