(Linear) (Six-base) MAPSORT of: KIEE3920.seq  Check: 7541  from: 1  to: 816



KIEE3920.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:51  ..

AccI GT'mk_AC

Cuts at:      0     152     816 
   Size:        152     664
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     425     719     764     816 
   Size:        229     196     294      45      52

  Fragments arranged by size: 

                 294     229     196      52      45
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      95     816 
   Size:         95     721
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     816 
   Size:         15     801
 
ApoI r'AATT_y

Cuts at:      0     121     223     816 
   Size:        121     102     593

  Fragments arranged by size: 

                 593     121     102
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     707     816 
   Size:        707     109
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     272     816 
   Size:        272     544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     816 
   Size:        590     226
 
Bpu10I CC'TnA_GC

Cuts at:      0     343     816 
   Size:        343     473
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     816 
   Size:        341     475
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     107     816 
   Size:        107     709
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     464     816 
   Size:        464     352
 
BsiHKAI G_wGCw'C

Cuts at:      0     752     816 
   Size:        752      64
 
BsmI GAATG_Cn'

Cuts at:      0     718     816 
   Size:        718      98
 
Bsp1286I G_dGCh'C

Cuts at:      0     752     816 
   Size:        752      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     311     327     816 
   Size:        311      16     489

  Fragments arranged by size: 

                 489     311      16
 
BtsI GCAGTG_nn'

Cuts at:      0     493     816 
   Size:        493     323
 
EaeI y'GGCC_r

Cuts at:      0     138     611     816 
   Size:        138     473     205

  Fragments arranged by size: 

                 473     205     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     425     590     719     764     816 
   Size:        229     196     165     129      45      52

  Fragments arranged by size: 

                 229     196     165     129      52      45
 
EcoRI G'AATT_C

Cuts at:      0     121     816 
   Size:        121     695
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     530     681     816 
   Size:        530     151     135

  Fragments arranged by size: 

                 530     151     135
 
HaeII r_GCGC'y

Cuts at:      0     448     816 
   Size:        448     368
 
HindIII A'AGCT_T

Cuts at:      0     359     816 
   Size:        359     457
 
MfeI C'AATT_G

Cuts at:      0     395     816 
   Size:        395     421
 
MscI TGG'CCA

Cuts at:      0     140     613     816 
   Size:        140     473     203

  Fragments arranged by size: 

                 473     203     140
 
NspI r_CATG'y

Cuts at:      0     102     816 
   Size:        102     714
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     525     816 
   Size:        525     291
 
SexAI A'CCwGG_T

Cuts at:      0     524     816 
   Size:        524     292
 
SfcI C'TryA_G

Cuts at:      0     307     717     816 
   Size:        307     410      99

  Fragments arranged by size: 

                 410     307      99
 
SmlI C'TyrA_G

Cuts at:      0     356     816 
   Size:        356     460
 
XmnI GAAnn'nnTTC

Cuts at:      0      43     665     816 
   Size:         43     622     151

  Fragments arranged by size: 

                 622     151      43
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     BbsI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI
     BtsI     EaeI  Eco57MI    EcoRI     FalI    HaeII  HindIII     MfeI
     MscI     NspI    PflMI    SexAI     SfcI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI