(Linear) (Six-base) MAPSORT of: KIEE3914.seq  Check: 8306  from: 1  to: 681



KIEE3914.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:49  ..

AatII G_ACGT'C

Cuts at:      0     465     681 
   Size:        465     216
 
AccI GT'mk_AC

Cuts at:      0     459     681 
   Size:        459     222
 
AseI AT'TA_AT

Cuts at:      0     328     681 
   Size:        328     353
 
AvaI C'yCGr_G

Cuts at:      0     559     681 
   Size:        559     122
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     449     483     681 
   Size:        449      34     198

  Fragments arranged by size: 

                 449     198      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     681 
   Size:        511     170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     681 
   Size:        430     251
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     181     681 
   Size:        181     500
 
BsaHI Gr'CG_yC

Cuts at:      0     462     681 
   Size:        462     219
 
BsaWI w'CCGG_w

Cuts at:      0     635     681 
   Size:        635      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     539     569     681 
   Size:        539      30     112

  Fragments arranged by size: 

                 539     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     681 
   Size:        304     377
 
BseYI C'CCAG_C

Cuts at:      0      10      34     238     681 
   Size:         10      24     204     443

  Fragments arranged by size: 

                 443     204      24      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     558     681 
   Size:        520      38     123

  Fragments arranged by size: 

                 520     123      38
 
BsiHKAI G_wGCw'C

Cuts at:      0     679     681 
   Size:        679       2
 
Bsp1286I G_dGCh'C

Cuts at:      0     679     681 
   Size:        679       2
 
BspHI T'CATG_A

Cuts at:      0     614     681 
   Size:        614      67
 
BsrBI CCG'CTC

Cuts at:      0     145     681 
   Size:        145     536
 
BstYI r'GATC_y

Cuts at:      0     346     681 
   Size:        346     335
 
BtgI C'CryG_G

Cuts at:      0     594     681 
   Size:        594      87
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     535     681 
   Size:        535     146
 
EaeI y'GGCC_r

Cuts at:      0     397     597     681 
   Size:        397     200      84

  Fragments arranged by size: 

                 397     200      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     448     681 
   Size:        179     269     233

  Fragments arranged by size: 

                 269     233     179
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     681 
   Size:        511     170
 
HaeII r_GCGC'y

Cuts at:      0      34     651     681 
   Size:         34     617      30

  Fragments arranged by size: 

                 617      34      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      72     104     352     384     447     479     681 
   Size:         72      32     248      32      63      32     202

  Fragments arranged by size: 

                 248     202      72      63      32      32      32
 
HincII GTy'rAC

Cuts at:      0     460     681 
   Size:        460     221
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     681 
   Size:        280     401
 
MslI CAynn'nnrTG

Cuts at:      0     619     681 
   Size:        619      62
 
RsrII CG'GwC_CG

Cuts at:      0     425     681 
   Size:        425     256
 
SalI G'TCGA_C

Cuts at:      0     458     681 
   Size:        458     223
 
SmlI C'TyrA_G

Cuts at:      0     409     681 
   Size:        409     272
 
TatI w'GTAC_w

Cuts at:      0     151     681 
   Size:        151     530
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     492     592     618     681 
   Size:        492     100      26      63

  Fragments arranged by size: 

                 492     100      63      26
 
ZraI GAC'GTC

Cuts at:      0     463     681 
   Size:        463     218
 

 Enzymes that do cut:

    AatII     AccI     AseI     AvaI     BcgI     BpmI    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrBI    BstYI     BtgI    BtgZI     EaeI     EarI  Eco57MI
    HaeII    Hin4I   HincII     MmeI     MslI    RsrII     SalI     SmlI
     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII     SacI    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI