Restriction map for
pFN21AE3898
(Linear) (Six-base) MAPSORT of: KIEE3898.seq Check: 4756 from: 1 to: 927
KIEE3898.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AatII G_ACGT'C
Cuts at: 0 496 927
Size: 496 431
AccI GT'mk_AC
Cuts at: 0 461 927
Size: 461 466
Acc65I G'GTAC_C
Cuts at: 0 521 927
Size: 521 406
AlwNI CAG_nnn'CTG
Cuts at: 0 87 927
Size: 87 840
BamHI G'GATC_C
Cuts at: 0 774 927
Size: 774 153
BanI G'GyrC_C
Cuts at: 0 9 521 927
Size: 9 512 406
Fragments arranged by size:
512 406 9
BanII G_rGCy'C
Cuts at: 0 728 927
Size: 728 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 157 341 927
Size: 157 184 586
Fragments arranged by size:
586 184 157
BclI T'GATC_A
Cuts at: 0 422 554 927
Size: 422 132 373
Fragments arranged by size:
422 373 132
BlpI GC'TnA_GC
Cuts at: 0 370 927
Size: 370 557
Bme1580I G_kGCm'C
Cuts at: 0 770 927
Size: 770 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 459 927
Size: 459 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 417 568 927
Size: 119 298 151 359
Fragments arranged by size:
359 298 151 119
BsaAI yAC'GTr
Cuts at: 0 330 927
Size: 330 597
BsaHI Gr'CG_yC
Cuts at: 0 493 824 927
Size: 493 331 103
Fragments arranged by size:
493 331 103
BsaWI w'CCGG_w
Cuts at: 0 114 518 593 927
Size: 114 404 75 334
Fragments arranged by size:
404 334 114 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 927
Size: 715 30 182
Fragments arranged by size:
715 182 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 745 927
Size: 19 726 182
Fragments arranged by size:
726 182 19
BseYI C'CCAG_C
Cuts at: 0 837 927
Size: 837 90
BsiEI CG_ry'CG
Cuts at: 0 515 927
Size: 515 412
BsiHKAI G_wGCw'C
Cuts at: 0 728 927
Size: 728 199
Bsp1286I G_dGCh'C
Cuts at: 0 728 770 927
Size: 728 42 157
Fragments arranged by size:
728 157 42
BspEI T'CCGG_A
Cuts at: 0 114 593 927
Size: 114 479 334
Fragments arranged by size:
479 334 114
BsrBI CCG'CTC
Cuts at: 0 103 780 927
Size: 103 677 147
Fragments arranged by size:
677 147 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 927
Size: 306 621
BstYI r'GATC_y
Cuts at: 0 774 927
Size: 774 153
BtgI C'CryG_G
Cuts at: 0 228 927
Size: 228 699
BtsI GCAGTG_nn'
Cuts at: 0 557 927
Size: 557 370
ClaI AT'CG_AT
Cuts at: 0 275 292 542 927
Size: 275 17 250 385
Fragments arranged by size:
385 275 250 17
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 927
Size: 745 182
EaeI y'GGCC_r
Cuts at: 0 527 927
Size: 527 400
EarI CTCTTCn'nnn_
Cuts at: 0 269 902 927
Size: 269 633 25
Fragments arranged by size:
633 269 25
EcoICRI GAG'CTC
Cuts at: 0 726 927
Size: 726 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 417 568 927
Size: 119 298 151 359
Fragments arranged by size:
359 298 151 119
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 927
Size: 238 689
EcoO109I rG'GnC_Cy
Cuts at: 0 403 747 927
Size: 403 344 180
Fragments arranged by size:
403 344 180
HaeII r_GCGC'y
Cuts at: 0 41 218 927
Size: 41 177 709
Fragments arranged by size:
709 177 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 539 571 687 719 927
Size: 539 32 116 32 208
Fragments arranged by size:
539 208 116 32 32
HincII GTy'rAC
Cuts at: 0 462 927
Size: 462 465
KpnI G_GTAC'C
Cuts at: 0 525 927
Size: 525 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 927
Size: 476 451
MscI TGG'CCA
Cuts at: 0 529 927
Size: 529 398
MslI CAynn'nnrTG
Cuts at: 0 542 640 927
Size: 542 98 287
Fragments arranged by size:
542 287 98
MspA1I CmG'CkG
Cuts at: 0 84 927
Size: 84 843
NcoI C'CATG_G
Cuts at: 0 228 927
Size: 228 699
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 927
Size: 741 186
PfoI T'CCnGG_A
Cuts at: 0 584 927
Size: 584 343
PpuMI rG'GwC_Cy
Cuts at: 0 403 747 927
Size: 403 344 180
Fragments arranged by size:
403 344 180
SacI G_AGCT'C
Cuts at: 0 728 927
Size: 728 199
SalI G'TCGA_C
Cuts at: 0 460 927
Size: 460 467
SanDI GG'GwC_CC
Cuts at: 0 403 927
Size: 403 524
SapI GCTCTTCn'nnn_
Cuts at: 0 269 927
Size: 269 658
SfcI C'TryA_G
Cuts at: 0 125 852 927
Size: 125 727 75
Fragments arranged by size:
727 125 75
StyI C'CwwG_G
Cuts at: 0 228 927
Size: 228 699
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 501 927
Size: 501 426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 177 209 715 747 927
Size: 177 32 506 32 180
Fragments arranged by size:
506 180 177 32 32
XbaI T'CTAG_A
Cuts at: 0 267 927
Size: 267 660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 639 927
Size: 226 413 288
Fragments arranged by size:
413 288 226
ZraI GAC'GTC
Cuts at: 0 494 927
Size: 494 433
Enzymes that do cut:
AatII AccI Acc65I AlwNI BamHI BanI BanII BbsI
BclI BlpI Bme1580I BmrI BpmI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BsrBI
BstXI BstYI BtgI BtsI ClaI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NcoI PflMI PfoI PpuMI
SacI SalI SanDI SapI SfcI StyI TaqII TstI
XbaI XcmI ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsgI BsiWI BsmI BsmBI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XhoI XmaI
XmnI