(Linear) (Six-base) MAPSORT of: KIEE3897.seq  Check: 7953  from: 1  to: 579



KIEE3897.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     317     476     579 
   Size:        181     136     159     103

  Fragments arranged by size: 

                 181     159     136     103
 
AflIII A'CryG_T

Cuts at:      0     311     579 
   Size:        311     268
 
AseI AT'TA_AT

Cuts at:      0     370     579 
   Size:        370     209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     467     579 
   Size:        467     112
 
BglII A'GATC_T

Cuts at:      0      11     579 
   Size:         11     568
 
Bme1580I G_kGCm'C

Cuts at:      0     564     579 
   Size:        564      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     218     579 
   Size:        193      25     361

  Fragments arranged by size: 

                 361     193      25
 
BsaBI GATnn'nnATC

Cuts at:      0     221     579 
   Size:        221     358
 
BsaWI w'CCGG_w

Cuts at:      0      54     579 
   Size:         54     525
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     166     196     579 
   Size:        166      30     383

  Fragments arranged by size: 

                 383     166      30
 
BsiEI CG_ry'CG

Cuts at:      0      77     579 
   Size:         77     502
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     579 
   Size:         35     544
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     564     579 
   Size:         35     529      15

  Fragments arranged by size: 

                 529      35      15
 
BsrBI CCG'CTC

Cuts at:      0      46     579 
   Size:         46     533
 
BsrFI r'CCGG_y

Cuts at:      0      76     124     579 
   Size:         76      48     455

  Fragments arranged by size: 

                 455      76      48
 
BstYI r'GATC_y

Cuts at:      0      11     579 
   Size:         11     568
 
EaeI y'GGCC_r

Cuts at:      0      41      74     126     579 
   Size:         41      33      52     453

  Fragments arranged by size: 

                 453      52      41      33
 
EagI C'GGCC_G

Cuts at:      0      74     579 
   Size:         74     505
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     193     218     317     476     579 
   Size:        181      12      25      99     159     103

  Fragments arranged by size: 

                 181     159     103      99      25      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     156     579 
   Size:        156     423
 
NaeI GCC'GGC

Cuts at:      0      78     126     579 
   Size:         78      48     453

  Fragments arranged by size: 

                 453      78      48
 
NgoMIV G'CCGG_C

Cuts at:      0      76     124     579 
   Size:         76      48     455

  Fragments arranged by size: 

                 455      76      48
 
NspI r_CATG'y

Cuts at:      0     315     579 
   Size:        315     264
 
PciI A'CATG_T

Cuts at:      0     311     579 
   Size:        311     268
 
PfoI T'CCnGG_A

Cuts at:      0     374     467     579 
   Size:        374      93     112

  Fragments arranged by size: 

                 374     112      93
 
PsiI TTA'TAA

Cuts at:      0     481     579 
   Size:        481      98
 
SfcI C'TryA_G

Cuts at:      0     287     579 
   Size:        287     292
 
StuI AGG'CCT

Cuts at:      0     166     579 
   Size:        166     413
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     193     579 
   Size:        193     386
 

 Enzymes that do cut:

     AcuI   AflIII     AseI     BbsI    BglII Bme1580I     BpmI    BsaBI
    BsaWI    BsaXI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrFI    BstYI
     EaeI     EagI  Eco57MI     FalI     NaeI   NgoMIV     NspI     PciI
     PfoI     PsiI     SfcI     StuI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI