Restriction map for
pFN21AE3896
(Linear) (Six-base) MAPSORT of: KIEE3896.seq Check: 7569 from: 1 to: 975
KIEE3896.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AclI AA'CG_TT
Cuts at: 0 821 975
Size: 821 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 975
Size: 866 109
AlwNI CAG_nnn'CTG
Cuts at: 0 426 504 975
Size: 426 78 471
Fragments arranged by size:
471 426 78
ApaI G_GGCC'C
Cuts at: 0 441 975
Size: 441 534
ApoI r'AATT_y
Cuts at: 0 251 975
Size: 251 724
AvaI C'yCGr_G
Cuts at: 0 440 975
Size: 440 535
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 587 620 975
Size: 587 33 355
Fragments arranged by size:
587 355 33
BanI G'GyrC_C
Cuts at: 0 404 975
Size: 404 571
BanII G_rGCy'C
Cuts at: 0 441 975
Size: 441 534
BglI GCCn_nnn'nGGC
Cuts at: 0 421 493 975
Size: 421 72 482
Fragments arranged by size:
482 421 72
Bme1580I G_kGCm'C
Cuts at: 0 441 975
Size: 441 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 975
Size: 84 891
BsaAI yAC'GTr
Cuts at: 0 118 975
Size: 118 857
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 975
Size: 82 30 863
Fragments arranged by size:
863 82 30
BseYI C'CCAG_C
Cuts at: 0 581 975
Size: 581 394
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 975
Size: 439 536
BsiHKAI G_wGCw'C
Cuts at: 0 944 975
Size: 944 31
BsmI GAATG_Cn'
Cuts at: 0 111 975
Size: 111 864
BsmBI CGTCTCn'nnnn_
Cuts at: 0 617 975
Size: 617 358
Bsp1286I G_dGCh'C
Cuts at: 0 441 944 975
Size: 441 503 31
Fragments arranged by size:
503 441 31
BspHI T'CATG_A
Cuts at: 0 306 975
Size: 306 669
BsrBI CCG'CTC
Cuts at: 0 705 975
Size: 705 270
BsrDI GCAATG_nn'
Cuts at: 0 122 975
Size: 122 853
BsrFI r'CCGG_y
Cuts at: 0 59 975
Size: 59 916
BsrGI T'GTAC_A
Cuts at: 0 729 975
Size: 729 246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 504 975
Size: 426 78 471
Fragments arranged by size:
471 426 78
BstYI r'GATC_y
Cuts at: 0 186 577 975
Size: 186 391 398
Fragments arranged by size:
398 391 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 790 975
Size: 790 185
DraI TTT'AAA
Cuts at: 0 320 975
Size: 320 655
EaeI y'GGCC_r
Cuts at: 0 2 217 267 296 975
Size: 2 215 50 29 679
Fragments arranged by size:
679 215 50 29 2
EarI CTCTTCn'nnn_
Cuts at: 0 344 400 695 975
Size: 344 56 295 280
Fragments arranged by size:
344 295 280 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 866 975
Size: 84 782 109
Fragments arranged by size:
782 109 84
EcoO109I rG'GnC_Cy
Cuts at: 0 423 501 975
Size: 423 78 474
Fragments arranged by size:
474 423 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 975
Size: 562 32 381
Fragments arranged by size:
562 381 32
MscI TGG'CCA
Cuts at: 0 4 298 975
Size: 4 294 677
Fragments arranged by size:
677 294 4
MslI CAynn'nnrTG
Cuts at: 0 691 937 975
Size: 691 246 38
Fragments arranged by size:
691 246 38
MspA1I CmG'CkG
Cuts at: 0 66 975
Size: 66 909
NspI r_CATG'y
Cuts at: 0 361 975
Size: 361 614
PspOMI G'GGCC_C
Cuts at: 0 437 975
Size: 437 538
PstI C_TGCA'G
Cuts at: 0 509 975
Size: 509 466
PvuII CAG'CTG
Cuts at: 0 66 975
Size: 66 909
SapI GCTCTTCn'nnn_
Cuts at: 0 400 695 975
Size: 400 295 280
Fragments arranged by size:
400 295 280
SbfI CC_TGCA'GG
Cuts at: 0 509 975
Size: 509 466
SfcI C'TryA_G
Cuts at: 0 505 975
Size: 505 470
SmaI CCC'GGG
Cuts at: 0 442 975
Size: 442 533
SrfI GCCC'GGGC
Cuts at: 0 442 975
Size: 442 533
SspI AAT'ATT
Cuts at: 0 758 920 975
Size: 758 162 55
Fragments arranged by size:
758 162 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 975
Size: 222 753
TatI w'GTAC_w
Cuts at: 0 729 975
Size: 729 246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 975
Size: 82 32 861
Fragments arranged by size:
861 82 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 227 975
Size: 227 748
XmaI C'CCGG_G
Cuts at: 0 440 975
Size: 440 535
Enzymes that do cut:
AclI AcuI AlwNI ApaI ApoI AvaI BaeI BanI
BanII BglI Bme1580I BpmI BsaAI BsaXI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI
BsrGI BstAPI BstYI BtgZI DraI EaeI EarI Eco57MI
EcoO109I Hin4I MscI MslI MspA1I NspI PspOMI PstI
PvuII SapI SbfI SfcI SmaI SrfI SspI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BspEI BspMI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
StuI StyI SwaI Tth111I XbaI XhoI XmnI ZraI