Restriction map for
pFN21AE3894
(Linear) (Six-base) MAPSORT of: KIEE3894.seq Check: 9446 from: 1 to: 927
KIEE3894.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
Acc65I G'GTAC_C
Cuts at: 0 645 927
Size: 645 282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 617 927
Size: 128 489 310
Fragments arranged by size:
489 310 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 533 927
Size: 533 394
ApaI G_GGCC'C
Cuts at: 0 18 627 835 927
Size: 18 609 208 92
Fragments arranged by size:
609 208 92 18
AvaI C'yCGr_G
Cuts at: 0 400 927
Size: 400 527
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 637 670 927
Size: 637 33 257
Fragments arranged by size:
637 257 33
BanI G'GyrC_C
Cuts at: 0 142 645 927
Size: 142 503 282
Fragments arranged by size:
503 282 142
BanII G_rGCy'C
Cuts at: 0 18 627 763 835 927
Size: 18 609 136 72 92
Fragments arranged by size:
609 136 92 72 18
BbeI G_GCGC'C
Cuts at: 0 146 927
Size: 146 781
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 809 927
Size: 230 579 118
Fragments arranged by size:
579 230 118
Bme1580I G_kGCm'C
Cuts at: 0 18 627 835 927
Size: 18 609 208 92
Fragments arranged by size:
609 208 92 18
BmgBI CAC'GTC
Cuts at: 0 294 927
Size: 294 633
BmrI ACTGGGnnnn_n'
Cuts at: 0 864 873 927
Size: 864 9 54
Fragments arranged by size:
864 54 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 695 927
Size: 121 574 232
Fragments arranged by size:
574 232 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 927
Size: 361 566
BsaHI Gr'CG_yC
Cuts at: 0 143 927
Size: 143 784
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 668 698 927
Size: 60 30 578 30 229
Fragments arranged by size:
578 229 60 30 30
BseYI C'CCAG_C
Cuts at: 0 176 906 927
Size: 176 730 21
Fragments arranged by size:
730 176 21
BsiEI CG_ry'CG
Cuts at: 0 11 131 927
Size: 11 120 796
Fragments arranged by size:
796 120 11
Bsp1286I G_dGCh'C
Cuts at: 0 18 627 763 835 927
Size: 18 609 136 72 92
Fragments arranged by size:
609 136 92 72 18
BsrBI CCG'CTC
Cuts at: 0 738 927
Size: 738 189
BsrFI r'CCGG_y
Cuts at: 0 165 927
Size: 165 762
BsrGI T'GTAC_A
Cuts at: 0 662 927
Size: 662 265
BssSI C'ACGA_G
Cuts at: 0 149 927
Size: 149 778
BstXI CCAn_nnnn'nTGG
Cuts at: 0 184 450 927
Size: 184 266 477
Fragments arranged by size:
477 266 184
BstYI r'GATC_y
Cuts at: 0 338 927
Size: 338 589
BtgI C'CryG_G
Cuts at: 0 113 755 836 927
Size: 113 642 81 91
Fragments arranged by size:
642 113 91 81
DraI TTT'AAA
Cuts at: 0 699 927
Size: 699 228
EaeI y'GGCC_r
Cuts at: 0 8 128 927
Size: 8 120 799
Fragments arranged by size:
799 120 8
EagI C'GGCC_G
Cuts at: 0 8 128 927
Size: 8 120 799
Fragments arranged by size:
799 120 8
EarI CTCTTCn'nnn_
Cuts at: 0 524 927
Size: 524 403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 128 617 695 927
Size: 121 7 489 78 232
Fragments arranged by size:
489 232 121 78 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 791 927
Size: 791 136
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 189 623 832 927
Size: 14 1 174 434 209 95
Fragments arranged by size:
434 209 174 95 14 1
FspI TGC'GCA
Cuts at: 0 509 927
Size: 509 418
HaeII r_GCGC'y
Cuts at: 0 146 636 927
Size: 146 490 291
Fragments arranged by size:
490 291 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 927
Size: 339 32 556
Fragments arranged by size:
556 339 32
HincII GTy'rAC
Cuts at: 0 93 927
Size: 93 834
KasI G'GCGC_C
Cuts at: 0 142 927
Size: 142 785
KpnI G_GTAC'C
Cuts at: 0 649 927
Size: 649 278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 895 927
Size: 560 335 32
Fragments arranged by size:
560 335 32
MspA1I CmG'CkG
Cuts at: 0 115 651 717 927
Size: 115 536 66 210
Fragments arranged by size:
536 210 115 66
NaeI GCC'GGC
Cuts at: 0 167 927
Size: 167 760
NarI GG'CG_CC
Cuts at: 0 143 927
Size: 143 784
NcoI C'CATG_G
Cuts at: 0 755 927
Size: 755 172
NgoMIV G'CCGG_C
Cuts at: 0 165 927
Size: 165 762
NspI r_CATG'y
Cuts at: 0 663 927
Size: 663 264
PasI CC'CwG_GG
Cuts at: 0 184 927
Size: 184 743
PflMI CCAn_nnn'nTGG
Cuts at: 0 816 927
Size: 816 111
PpuMI rG'GwC_Cy
Cuts at: 0 189 927
Size: 189 738
PspOMI G'GGCC_C
Cuts at: 0 14 623 831 927
Size: 14 609 208 96
Fragments arranged by size:
609 208 96 14
PstI C_TGCA'G
Cuts at: 0 164 556 927
Size: 164 392 371
Fragments arranged by size:
392 371 164
PvuII CAG'CTG
Cuts at: 0 717 927
Size: 717 210
SacII CC_GC'GG
Cuts at: 0 116 927
Size: 116 811
SanDI GG'GwC_CC
Cuts at: 0 189 927
Size: 189 738
SfcI C'TryA_G
Cuts at: 0 160 552 927
Size: 160 392 375
Fragments arranged by size:
392 375 160
SfoI GGC'GCC
Cuts at: 0 144 927
Size: 144 783
StuI AGG'CCT
Cuts at: 0 205 927
Size: 205 722
StyI C'CwwG_G
Cuts at: 0 755 927
Size: 755 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 198 510 846 927
Size: 198 312 336 81
Fragments arranged by size:
336 312 198 81
TatI w'GTAC_w
Cuts at: 0 662 860 927
Size: 662 198 67
Fragments arranged by size:
662 198 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 668 700 927
Size: 668 32 227
Fragments arranged by size:
668 227 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 721 771 927
Size: 450 271 50 156
Fragments arranged by size:
450 271 156 50
Enzymes that do cut:
Acc65I AcuI AlfI ApaI AvaI BaeI BanI BanII
BbeI BbsI Bme1580I BmgBI BmrI BpmI BsaI BsaHI
BsaXI BseYI BsiEI Bsp1286I BsrBI BsrFI BsrGI BssSI
BstXI BstYI BtgI DraI EaeI EagI EarI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI
MmeI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PpuMI PspOMI PstI PvuII SacII SanDI SfcI
SfoI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI MfeI MluI MscI MslI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI