Restriction map for
pFN21AE3891
(Linear) (Six-base) MAPSORT of: KIEE3891.seq Check: 6683 from: 1 to: 1029
KIEE3891.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1029
Size: 226 803
AfeI AGC'GCT
Cuts at: 0 454 1029
Size: 454 575
AflIII A'CryG_T
Cuts at: 0 675 1029
Size: 675 354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 383 1029
Size: 383 646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 911 943 1029
Size: 911 32 86
Fragments arranged by size:
911 86 32
ApoI r'AATT_y
Cuts at: 0 556 598 1029
Size: 556 42 431
Fragments arranged by size:
556 431 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 232 1029
Size: 196 36 797
Fragments arranged by size:
797 196 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 872 889 1029
Size: 872 17 140
Fragments arranged by size:
872 140 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1029
Size: 1004 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 496 1029
Size: 496 533
BsaAI yAC'GTr
Cuts at: 0 676 1029
Size: 676 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 911 941 1029
Size: 911 30 88
Fragments arranged by size:
911 88 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 374 1029
Size: 262 112 655
Fragments arranged by size:
655 262 112
BseYI C'CCAG_C
Cuts at: 0 166 419 1029
Size: 166 253 610
Fragments arranged by size:
610 253 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1029
Size: 353 676
BtsI GCAGTG_nn'
Cuts at: 0 188 583 1029
Size: 188 395 446
Fragments arranged by size:
446 395 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1004 1029
Size: 226 778 25
Fragments arranged by size:
778 226 25
EcoRI G'AATT_C
Cuts at: 0 556 1029
Size: 556 473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 576 1029
Size: 507 69 453
Fragments arranged by size:
507 453 69
HaeII r_GCGC'y
Cuts at: 0 456 671 1029
Size: 456 215 358
Fragments arranged by size:
456 358 215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 1029
Size: 676 32 321
Fragments arranged by size:
676 321 32
MfeI C'AATT_G
Cuts at: 0 785 1029
Size: 785 244
MspA1I CmG'CkG
Cuts at: 0 717 975 1029
Size: 717 258 54
Fragments arranged by size:
717 258 54
PmlI CAC'GTG
Cuts at: 0 676 1029
Size: 676 353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 911 943 1029
Size: 911 32 86
Fragments arranged by size:
911 86 32
PsiI TTA'TAA
Cuts at: 0 622 1029
Size: 622 407
PstI C_TGCA'G
Cuts at: 0 183 336 1029
Size: 183 153 693
Fragments arranged by size:
693 183 153
PvuII CAG'CTG
Cuts at: 0 975 1029
Size: 975 54
ScaI AGT'ACT
Cuts at: 0 878 1029
Size: 878 151
SfcI C'TryA_G
Cuts at: 0 179 332 536 545 970 1029
Size: 179 153 204 9 425 59
Fragments arranged by size:
425 204 179 153 59 9
SspI AAT'ATT
Cuts at: 0 102 1029
Size: 102 927
StuI AGG'CCT
Cuts at: 0 38 1029
Size: 38 991
StyI C'CwwG_G
Cuts at: 0 777 1029
Size: 777 252
TatI w'GTAC_w
Cuts at: 0 876 1029
Size: 876 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 773 805 1029
Size: 773 32 224
Fragments arranged by size:
773 224 32
XmnI GAAnn'nnTTC
Cuts at: 0 443 490 1029
Size: 443 47 539
Fragments arranged by size:
539 443 47
Enzymes that do cut:
AcuI AfeI AflIII AlfI AloI ApoI BbsI BmrI
BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BtsI
Eco57MI EcoRI FalI HaeII Hin4I MfeI MspA1I PmlI
PpiI PsiI PstI PvuII ScaI SfcI SspI StuI
StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI