(Linear) (Six-base) MAPSORT of: KIEE3886.seq  Check: 8339  from: 1  to: 348



KIEE3886.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AccI GT'mk_AC

Cuts at:      0     139     348 
   Size:        139     209
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     229     348 
   Size:         34     195     119

  Fragments arranged by size: 

                 195     119      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      25     212     348 
   Size:         25     187     136

  Fragments arranged by size: 

                 187     136      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     348 
   Size:        124     224
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      47     348 
   Size:         47     301
 
BsrGI T'GTAC_A

Cuts at:      0      80     348 
   Size:         80     268
 
BssSI C'ACGA_G

Cuts at:      0      61     348 
   Size:         61     287
 
BstZ17I GTA'TAC

Cuts at:      0     140     348 
   Size:        140     208
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      12      47     348 
   Size:         12      35     301

  Fragments arranged by size: 

                 301      35      12
 
EaeI y'GGCC_r

Cuts at:      0     340     348 
   Size:        340       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     308     348 
   Size:        227      81      40

  Fragments arranged by size: 

                 227      81      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     229     348 
   Size:         34     195     119

  Fragments arranged by size: 

                 195     119      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     229     348 
   Size:        229     119
 
MscI TGG'CCA

Cuts at:      0     342     348 
   Size:        342       6
 
SapI GCTCTTCn'nnn_

Cuts at:      0     308     348 
   Size:        308      40
 
SmlI C'TyrA_G

Cuts at:      0     103     348 
   Size:        103     245
 
SspI AAT'ATT

Cuts at:      0      77     348 
   Size:         77     271
 
TatI w'GTAC_w

Cuts at:      0      80     348 
   Size:         80     268
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61     348 
   Size:         29      32     287

  Fragments arranged by size: 

                 287      32      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30     348 
   Size:         30     318
 

 Enzymes that do cut:

     AccI     AcuI     BbsI    BpuEI    BseRI    BsrGI    BssSI  BstZ17I
    CspCI     EaeI     EarI  Eco57MI     FalI     MscI     SapI     SmlI
     SspI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI