(Linear) (Six-base) MAPSORT of: KIEE3884.seq  Check: 1177  from: 1  to: 993



KIEE3884.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AflIII A'CryG_T

Cuts at:      0     911     993 
   Size:        911      82
 
AlwNI CAG_nnn'CTG

Cuts at:      0     508     993 
   Size:        508     485
 
BanI G'GyrC_C

Cuts at:      0      93     993 
   Size:         93     900
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     993 
   Size:         30     963
 
BbvCI CC'TCA_GC

Cuts at:      0     163     993 
   Size:        163     830
 
Bpu10I CC'TnA_GC

Cuts at:      0     163     993 
   Size:        163     830
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841     971     993 
   Size:        841     130      22

  Fragments arranged by size: 

                 841     130      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     386     993 
   Size:        386     607
 
BsmI GAATG_Cn'

Cuts at:      0     888     993 
   Size:        888     105
 
BsrDI GCAATG_nn'

Cuts at:      0      57     993 
   Size:         57     936
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     795     993 
   Size:        795     198
 
BstYI r'GATC_y

Cuts at:      0     122     519     687     993 
   Size:        122     397     168     306

  Fragments arranged by size: 

                 397     306     168     122
 
BtsI GCAGTG_nn'

Cuts at:      0     557     993 
   Size:        557     436
 
DraI TTT'AAA

Cuts at:      0     837     993 
   Size:        837     156
 
DraIII CAC_nnn'GTG

Cuts at:      0     924     993 
   Size:        924      69
 
EaeI y'GGCC_r

Cuts at:      0      17     993 
   Size:         17     976
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     449     993 
   Size:        449     544
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     156     188     297     329     389     421     993 
   Size:         26     130      32     109      32      60      32     572

  Fragments arranged by size: 

                 572     130     109      60      32      32      32      26
            
 
MluI A'CGCG_T

Cuts at:      0     911     993 
   Size:        911      82
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     572     993 
   Size:        513      59     421

  Fragments arranged by size: 

                 513     421      59
 
MscI TGG'CCA

Cuts at:      0      19     993 
   Size:         19     974
 
MslI CAynn'nnrTG

Cuts at:      0     875     993 
   Size:        875     118
 
MspA1I CmG'CkG

Cuts at:      0     271     993 
   Size:        271     722
 
NdeI CA'TA_TG

Cuts at:      0     808     993 
   Size:        808     185
 
PsiI TTA'TAA

Cuts at:      0     662     993 
   Size:        662     331
 
PstI C_TGCA'G

Cuts at:      0     305     933     993 
   Size:        305     628      60

  Fragments arranged by size: 

                 628     305      60
 
SfcI C'TryA_G

Cuts at:      0     301     929     993 
   Size:        301     628      64

  Fragments arranged by size: 

                 628     301      64
 
SmlI C'TyrA_G

Cuts at:      0     856     986     993 
   Size:        856     130       7

  Fragments arranged by size: 

                 856     130       7
 
SspI AAT'ATT

Cuts at:      0     277     683     993 
   Size:        277     406     310

  Fragments arranged by size: 

                 406     310     277
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      78     595     782     993 
   Size:         78     517     187     211

  Fragments arranged by size: 

                 517     211     187      78
 
TatI w'GTAC_w

Cuts at:      0      51     993 
   Size:         51     942
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     722     993 
   Size:        278     444     271

  Fragments arranged by size: 

                 444     278     271
 

 Enzymes that do cut:

   AflIII    AlwNI     BanI     BbsI    BbvCI   Bpu10I    BpuEI    BseRI
     BsmI    BsrDI    BstXI    BstYI     BtsI     DraI   DraIII     EaeI
    EcoNI    Hin4I     MluI     MmeI     MscI     MslI   MspA1I     NdeI
     PsiI     PstI     SfcI     SmlI     SspI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI