Restriction map for
pFN21AE3881
(Linear) (Six-base) MAPSORT of: KIEE3881.seq Check: 4181 from: 1 to: 1356
KIEE3881.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1199 1356
Size: 955 244 157
Fragments arranged by size:
955 244 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 837 1356
Size: 837 519
AlwNI CAG_nnn'CTG
Cuts at: 0 1275 1356
Size: 1275 81
ApoI r'AATT_y
Cuts at: 0 386 590 1356
Size: 386 204 766
Fragments arranged by size:
766 386 204
AseI AT'TA_AT
Cuts at: 0 209 1356
Size: 209 1147
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 1356
Size: 67 1289
BglII A'GATC_T
Cuts at: 0 1126 1356
Size: 1126 230
Bpu10I CC'TnA_GC
Cuts at: 0 902 1356
Size: 902 454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1356
Size: 1333 23
BsaBI GATnn'nnATC
Cuts at: 0 35 1356
Size: 35 1321
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1356
Size: 1244 112
BsiHKAI G_wGCw'C
Cuts at: 0 530 1356
Size: 530 826
BsmI GAATG_Cn'
Cuts at: 0 906 1175 1356
Size: 906 269 181
Fragments arranged by size:
906 269 181
Bsp1286I G_dGCh'C
Cuts at: 0 530 1356
Size: 530 826
BspHI T'CATG_A
Cuts at: 0 1151 1356
Size: 1151 205
BsrBI CCG'CTC
Cuts at: 0 720 1356
Size: 720 636
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1275 1356
Size: 1275 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 676 1356
Size: 676 680
BstYI r'GATC_y
Cuts at: 0 1126 1356
Size: 1126 230
BtsI GCAGTG_nn'
Cuts at: 0 1095 1356
Size: 1095 261
DraI TTT'AAA
Cuts at: 0 1115 1356
Size: 1115 241
DraIII CAC_nnn'GTG
Cuts at: 0 954 1356
Size: 954 402
EaeI y'GGCC_r
Cuts at: 0 435 666 678 1356
Size: 435 231 12 678
Fragments arranged by size:
678 435 231 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1199 1356
Size: 955 244 157
Fragments arranged by size:
955 244 157
EcoO109I rG'GnC_Cy
Cuts at: 0 566 1356
Size: 566 790
EcoRV GAT'ATC
Cuts at: 0 702 871 1356
Size: 702 169 485
Fragments arranged by size:
702 485 169
FspI TGC'GCA
Cuts at: 0 46 1356
Size: 46 1310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 961 993 1356
Size: 961 32 363
Fragments arranged by size:
961 363 32
MfeI C'AATT_G
Cuts at: 0 1328 1356
Size: 1328 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1182 1356
Size: 1182 174
MscI TGG'CCA
Cuts at: 0 437 668 680 1356
Size: 437 231 12 676
Fragments arranged by size:
676 437 231 12
MslI CAynn'nnrTG
Cuts at: 0 668 1356
Size: 668 688
NdeI CA'TA_TG
Cuts at: 0 1056 1356
Size: 1056 300
PstI C_TGCA'G
Cuts at: 0 1287 1356
Size: 1287 69
SfcI C'TryA_G
Cuts at: 0 227 561 1283 1356
Size: 227 334 722 73
Fragments arranged by size:
722 334 227 73
SmlI C'TyrA_G
Cuts at: 0 1312 1356
Size: 1312 44
SspI AAT'ATT
Cuts at: 0 1191 1356
Size: 1191 165
TatI w'GTAC_w
Cuts at: 0 145 1356
Size: 145 1211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 1356
Size: 936 32 388
Fragments arranged by size:
936 388 32
XbaI T'CTAG_A
Cuts at: 0 393 1356
Size: 393 963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1194 1356
Size: 1194 162
XmnI GAAnn'nnTTC
Cuts at: 0 63 464 1356
Size: 63 401 892
Fragments arranged by size:
892 401 63
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI BbsI BglII Bpu10I
BpuEI BsaBI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BstAPI BstXI BstYI BtsI DraI DraIII EaeI Eco57MI
EcoO109I EcoRV FspI Hin4I MfeI MmeI MscI MslI
NdeI PstI SfcI SmlI SspI TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI FalI FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII Tth111I XhoI
XmaI ZraI