Restriction map for
pFN21AE3880
(Linear) (Six-base) MAPSORT of: KIEE3880.seq Check: 4593 from: 1 to: 1137
KIEE3880.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 421 1137
Size: 421 716
Acc65I G'GTAC_C
Cuts at: 0 370 1137
Size: 370 767
AclI AA'CG_TT
Cuts at: 0 945 1137
Size: 945 192
AlwNI CAG_nnn'CTG
Cuts at: 0 563 687 1137
Size: 563 124 450
Fragments arranged by size:
563 450 124
ApoI r'AATT_y
Cuts at: 0 929 938 1137
Size: 929 9 199
Fragments arranged by size:
929 199 9
AvaI C'yCGr_G
Cuts at: 0 13 489 634 711 1137
Size: 13 476 145 77 426
Fragments arranged by size:
476 426 145 77 13
BamHI G'GATC_C
Cuts at: 0 740 1137
Size: 740 397
BanI G'GyrC_C
Cuts at: 0 297 370 409 1137
Size: 297 73 39 728
Fragments arranged by size:
728 297 73 39
BanII G_rGCy'C
Cuts at: 0 214 393 452 496 1137
Size: 214 179 59 44 641
Fragments arranged by size:
641 214 179 59 44
BbeI G_GCGC'C
Cuts at: 0 301 1137
Size: 301 836
BbvCI CC'TCA_GC
Cuts at: 0 469 1137
Size: 469 668
BglI GCCn_nnn'nGGC
Cuts at: 0 295 1137
Size: 295 842
BlpI GC'TnA_GC
Cuts at: 0 875 1137
Size: 875 262
BmrI ACTGGGnnnn_n'
Cuts at: 0 551 1137
Size: 551 586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 552 1137
Size: 552 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1137
Size: 547 590
Bpu10I CC'TnA_GC
Cuts at: 0 469 680 1137
Size: 469 211 457
Fragments arranged by size:
469 457 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 133 667 1137
Size: 88 45 534 470
Fragments arranged by size:
534 470 88 45
BsaBI GATnn'nnATC
Cuts at: 0 862 1137
Size: 862 275
BsaHI Gr'CG_yC
Cuts at: 0 298 1137
Size: 298 839
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 195 231 595 622 625 628 673 676
Size: 195 36 364 27 3 3 45 3
Cuts at: 676 685 1137
Size: 9 452
Fragments arranged by size:
452 364 195 45 36 27 9 3
3 3
BseYI C'CCAG_C
Cuts at: 0 159 451 1137
Size: 159 292 686
Fragments arranged by size:
686 292 159
BsiEI CG_ry'CG
Cuts at: 0 50 173 1137
Size: 50 123 964
Fragments arranged by size:
964 123 50
BsmI GAATG_Cn'
Cuts at: 0 546 1137
Size: 546 591
BsmBI CGTCTCn'nnnn_
Cuts at: 0 374 1137
Size: 374 763
Bsp1286I G_dGCh'C
Cuts at: 0 214 393 452 496 1137
Size: 214 179 59 44 641
Fragments arranged by size:
641 214 179 59 44
BspHI T'CATG_A
Cuts at: 0 360 1137
Size: 360 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 421 551 1137
Size: 421 130 586
Fragments arranged by size:
586 421 130
BsrBI CCG'CTC
Cuts at: 0 589 679 1137
Size: 589 90 458
Fragments arranged by size:
589 458 90
BsrFI r'CCGG_y
Cuts at: 0 406 436 460 1137
Size: 406 30 24 677
Fragments arranged by size:
677 406 30 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 479 1137
Size: 479 658
BstEII G'GTnAC_C
Cuts at: 0 321 1137
Size: 321 816
BstYI r'GATC_y
Cuts at: 0 399 740 1137
Size: 399 341 397
Fragments arranged by size:
399 397 341
Bsu36I CC'TnA_GG
Cuts at: 0 218 605 1137
Size: 218 387 532
Fragments arranged by size:
532 387 218
BtgI C'CryG_G
Cuts at: 0 206 245 344 977 1137
Size: 206 39 99 633 160
Fragments arranged by size:
633 206 160 99 39
DraI TTT'AAA
Cuts at: 0 950 1137
Size: 950 187
DraIII CAC_nnn'GTG
Cuts at: 0 42 1137
Size: 42 1095
EaeI y'GGCC_r
Cuts at: 0 47 170 347 806 1137
Size: 47 123 177 459 331
Fragments arranged by size:
459 331 177 123 47
EagI C'GGCC_G
Cuts at: 0 47 170 1137
Size: 47 123 967
Fragments arranged by size:
967 123 47
EarI CTCTTCn'nnn_
Cuts at: 0 440 749 1137
Size: 440 309 388
Fragments arranged by size:
440 388 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 151 1137
Size: 151 986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1137
Size: 547 590
EcoNI CCTnn'n_nnAGG
Cuts at: 0 305 1137
Size: 305 832
EcoO109I rG'GnC_Cy
Cuts at: 0 600 1137
Size: 600 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 1137
Size: 723 414
HaeII r_GCGC'y
Cuts at: 0 301 461 1137
Size: 301 160 676
Fragments arranged by size:
676 301 160
KasI G'GCGC_C
Cuts at: 0 297 1137
Size: 297 840
KpnI G_GTAC'C
Cuts at: 0 374 1137
Size: 374 763
MscI TGG'CCA
Cuts at: 0 808 1137
Size: 808 329
MspA1I CmG'CkG
Cuts at: 0 109 146 208 247 882 1137
Size: 109 37 62 39 635 255
Fragments arranged by size:
635 255 109 62 39 37
NaeI GCC'GGC
Cuts at: 0 408 438 462 1137
Size: 408 30 24 675
Fragments arranged by size:
675 408 30 24
NarI GG'CG_CC
Cuts at: 0 298 1137
Size: 298 839
NcoI C'CATG_G
Cuts at: 0 977 1137
Size: 977 160
NgoMIV G'CCGG_C
Cuts at: 0 406 436 460 1137
Size: 406 30 24 677
Fragments arranged by size:
677 406 30 24
NotI GC'GGCC_GC
Cuts at: 0 170 1137
Size: 170 967
NspI r_CATG'y
Cuts at: 0 909 1137
Size: 909 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 1068 1137
Size: 1068 69
PfoI T'CCnGG_A
Cuts at: 0 188 225 1137
Size: 188 37 912
Fragments arranged by size:
912 188 37
PpuMI rG'GwC_Cy
Cuts at: 0 600 1137
Size: 600 537
PstI C_TGCA'G
Cuts at: 0 26 238 1054 1137
Size: 26 212 816 83
Fragments arranged by size:
816 212 83 26
PvuII CAG'CTG
Cuts at: 0 882 1137
Size: 882 255
SacII CC_GC'GG
Cuts at: 0 209 248 1137
Size: 209 39 889
Fragments arranged by size:
889 209 39
SanDI GG'GwC_CC
Cuts at: 0 600 1137
Size: 600 537
SapI GCTCTTCn'nnn_
Cuts at: 0 440 1137
Size: 440 697
SfcI C'TryA_G
Cuts at: 0 22 88 234 997 1050 1137
Size: 22 66 146 763 53 87
Fragments arranged by size:
763 146 87 66 53 22
SfoI GGC'GCC
Cuts at: 0 299 1137
Size: 299 838
SgrAI Cr'CCGG_yG
Cuts at: 0 460 1137
Size: 460 677
SmaI CCC'GGG
Cuts at: 0 636 713 1137
Size: 636 77 424
Fragments arranged by size:
636 424 77
SpeI A'CTAG_T
Cuts at: 0 1065 1137
Size: 1065 72
SspI AAT'ATT
Cuts at: 0 892 1020 1137
Size: 892 128 117
Fragments arranged by size:
892 128 117
StuI AGG'CCT
Cuts at: 0 1007 1137
Size: 1007 130
StyI C'CwwG_G
Cuts at: 0 698 977 1137
Size: 698 279 160
Fragments arranged by size:
698 279 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 780 790 1137
Size: 780 10 347
Fragments arranged by size:
780 347 10
TatI w'GTAC_w
Cuts at: 0 314 1137
Size: 314 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 707 739 1137
Size: 707 32 398
Fragments arranged by size:
707 398 32
XmaI C'CCGG_G
Cuts at: 0 634 711 1137
Size: 634 77 426
Fragments arranged by size:
634 426 77
Enzymes that do cut:
AarI Acc65I AclI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbvCI BglI BlpI BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BseRI BseYI BsiEI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII
BstYI Bsu36I BtgI DraI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII KasI KpnI
MscI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PflMI PfoI PpuMI PstI PvuII SacII SanDI SapI
SfcI SfoI SgrAI SmaI SpeI SspI StuI StyI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII
Bme1580I BmgBI BmtI BpuEI BsaAI BsaWI BsaXI BsgI
BsiHKAI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I BtgZI BtsI ClaI CspCI DrdI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI MmeI MslI NdeI NheI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SphI
SrfI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI