Restriction map for
pFN21AE3878
(Linear) (Six-base) MAPSORT of: KIEE3878.seq Check: 9291 from: 1 to: 1896
KIEE3878.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1495 1896
Size: 1495 401
AatII G_ACGT'C
Cuts at: 0 1706 1896
Size: 1706 190
AccI GT'mk_AC
Cuts at: 0 1016 1589 1896
Size: 1016 573 307
Fragments arranged by size:
1016 573 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1476 1896
Size: 212 1264 420
Fragments arranged by size:
1264 420 212
AflIII A'CryG_T
Cuts at: 0 1509 1660 1896
Size: 1509 151 236
Fragments arranged by size:
1509 236 151
AhdI GACnn_n'nnGTC
Cuts at: 0 1616 1664 1896
Size: 1616 48 232
Fragments arranged by size:
1616 232 48
ApaI G_GGCC'C
Cuts at: 0 127 975 1896
Size: 127 848 921
Fragments arranged by size:
921 848 127
ApaLI G'TGCA_C
Cuts at: 0 112 1154 1896
Size: 112 1042 742
Fragments arranged by size:
1042 742 112
AvaI C'yCGr_G
Cuts at: 0 308 1042 1413 1896
Size: 308 734 371 483
Fragments arranged by size:
734 483 371 308
BamHI G'GATC_C
Cuts at: 0 71 1896
Size: 71 1825
BanI G'GyrC_C
Cuts at: 0 25 82 799 859 1072 1896
Size: 25 57 717 60 213 824
Fragments arranged by size:
824 717 213 60 57 25
BanII G_rGCy'C
Cuts at: 0 127 904 975 1041 1420 1896
Size: 127 777 71 66 379 476
Fragments arranged by size:
777 476 379 127 71 66
BbeI G_GCGC'C
Cuts at: 0 29 86 1896
Size: 29 57 1810
Fragments arranged by size:
1810 57 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1896
Size: 359 1537
BclI T'GATC_A
Cuts at: 0 713 1896
Size: 713 1183
BglII A'GATC_T
Cuts at: 0 872 1896
Size: 872 1024
Bme1580I G_kGCm'C
Cuts at: 0 116 127 328 804 975 1158 1896
Size: 116 11 201 476 171 183 738
Fragments arranged by size:
738 476 201 183 171 116 11
BmgBI CAC'GTC
Cuts at: 0 329 1554 1661 1896
Size: 329 1225 107 235
Fragments arranged by size:
1225 329 235 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 1073 1896
Size: 1073 823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 490 617 1778 1896
Size: 178 312 127 1161 118
Fragments arranged by size:
1161 312 178 127 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 1603 1896
Size: 1603 293
BsaHI Gr'CG_yC
Cuts at: 0 26 83 1703 1896
Size: 26 57 1620 193
Fragments arranged by size:
1620 193 57 26
BsaWI w'CCGG_w
Cuts at: 0 281 1268 1896
Size: 281 987 628
Fragments arranged by size:
987 628 281
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 898 928 1645 1675 1781 1811 1896
Size: 898 30 717 30 106 30 85
Fragments arranged by size:
898 717 106 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 906 1060 1669 1777 1780 1896
Size: 625 281 154 609 108 3 116
Fragments arranged by size:
625 609 281 154 116 108 3
BseYI C'CCAG_C
Cuts at: 0 182 586 1602 1896
Size: 182 404 1016 294
Fragments arranged by size:
1016 404 294 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1717 1866 1896
Size: 1717 149 30
Fragments arranged by size:
1717 149 30
BsiEI CG_ry'CG
Cuts at: 0 1718 1896
Size: 1718 178
BsiHKAI G_wGCw'C
Cuts at: 0 116 429 633 1158 1268 1896
Size: 116 313 204 525 110 628
Fragments arranged by size:
628 525 313 204 116 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1651 1896
Size: 1651 245
Bsp1286I G_dGCh'C
Cuts at: 0 116 127 328 429 633 804 904 975
Size: 116 11 201 101 204 171 100 71
Cuts at: 975 1041 1158 1268 1420 1896
Size: 66 117 110 152 476
Fragments arranged by size:
476 204 201 171 152 117 116 110
101 100 71 66 11
BspEI T'CCGG_A
Cuts at: 0 1268 1896
Size: 1268 628
BspHI T'CATG_A
Cuts at: 0 716 1896
Size: 716 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 352 968 1495 1896
Size: 352 616 527 401
Fragments arranged by size:
616 527 401 352
BsrDI GCAATG_nn'
Cuts at: 0 1100 1896
Size: 1100 796
BsrFI r'CCGG_y
Cuts at: 0 1207 1896
Size: 1207 689
BssHII G'CGCG_C
Cuts at: 0 41 1896
Size: 41 1855
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 1896
Size: 332 1564
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 1896
Size: 789 1107
BstYI r'GATC_y
Cuts at: 0 71 872 1896
Size: 71 801 1024
Fragments arranged by size:
1024 801 71
BtgI C'CryG_G
Cuts at: 0 77 474 1500 1613 1896
Size: 77 397 1026 113 283
Fragments arranged by size:
1026 397 283 113 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 374 415 1896
Size: 374 41 1481
Fragments arranged by size:
1481 374 41
BtsI GCAGTG_nn'
Cuts at: 0 755 1896
Size: 755 1141
DraI TTT'AAA
Cuts at: 0 695 1896
Size: 695 1201
DraIII CAC_nnn'GTG
Cuts at: 0 1674 1896
Size: 1674 222
EaeI y'GGCC_r
Cuts at: 0 408 759 1145 1556 1896
Size: 408 351 386 411 340
Fragments arranged by size:
411 408 386 351 340
EarI CTCTTCn'nnn_
Cuts at: 0 536 1514 1896
Size: 536 978 382
Fragments arranged by size:
978 536 382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 212 490 617 1476 1778 1896
Size: 178 34 278 127 859 302 118
Fragments arranged by size:
859 302 278 178 127 118 34
EcoO109I rG'GnC_Cy
Cuts at: 0 123 124 186 971 972 1896
Size: 123 1 62 785 1 924
Fragments arranged by size:
924 785 123 62 1 1
FspI TGC'GCA
Cuts at: 0 927 1085 1896
Size: 927 158 811
Fragments arranged by size:
927 811 158
FspAI rTGC'GCAy
Cuts at: 0 1085 1896
Size: 1085 811
HaeII r_GCGC'y
Cuts at: 0 29 58 86 108 1438 1896
Size: 29 29 28 22 1330 458
Fragments arranged by size:
1330 458 29 29 28 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 647 838 870 937 969 1206 1238
Size: 615 32 191 32 67 32 237 32
Cuts at: 1238 1263 1295 1602 1634 1650 1682 1896
Size: 25 32 307 32 16 32 214
Fragments arranged by size:
615 307 237 214 191 67 32 32
32 32 32 32 32 25 16
HincII GTy'rAC
Cuts at: 0 933 1896
Size: 933 963
KasI G'GCGC_C
Cuts at: 0 25 82 1896
Size: 25 57 1814
Fragments arranged by size:
1814 57 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1317 1376 1896
Size: 524 793 59 520
Fragments arranged by size:
793 524 520 59
MscI TGG'CCA
Cuts at: 0 761 1147 1558 1896
Size: 761 386 411 338
Fragments arranged by size:
761 411 386 338
MslI CAynn'nnrTG
Cuts at: 0 664 787 1202 1303 1390 1896
Size: 664 123 415 101 87 506
Fragments arranged by size:
664 506 415 123 101 87
MspA1I CmG'CkG
Cuts at: 0 79 130 757 1053 1641 1802 1880 1896
Size: 79 51 627 296 588 161 78 16
Fragments arranged by size:
627 588 296 161 79 78 51 16
NarI GG'CG_CC
Cuts at: 0 26 83 1896
Size: 26 57 1813
Fragments arranged by size:
1813 57 26
NcoI C'CATG_G
Cuts at: 0 474 1500 1613 1896
Size: 474 1026 113 283
Fragments arranged by size:
1026 474 283 113
NspI r_CATG'y
Cuts at: 0 1753 1896
Size: 1753 143
PacI TTA_AT'TAA
Cuts at: 0 869 1896
Size: 869 1027
PasI CC'CwG_GG
Cuts at: 0 1583 1896
Size: 1583 313
PpuMI rG'GwC_Cy
Cuts at: 0 186 1896
Size: 186 1710
PspOMI G'GGCC_C
Cuts at: 0 123 971 1896
Size: 123 848 925
Fragments arranged by size:
925 848 123
PspXI vC'TCGA_Gb
Cuts at: 0 308 1042 1896
Size: 308 734 854
Fragments arranged by size:
854 734 308
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 712 744 1896
Size: 712 32 1152
Fragments arranged by size:
1152 712 32
PstI C_TGCA'G
Cuts at: 0 1640 1896
Size: 1640 256
PvuI CG_AT'CG
Cuts at: 0 1718 1896
Size: 1718 178
PvuII CAG'CTG
Cuts at: 0 1053 1641 1880 1896
Size: 1053 588 239 16
Fragments arranged by size:
1053 588 239 16
SacII CC_GC'GG
Cuts at: 0 80 1896
Size: 80 1816
ScaI AGT'ACT
Cuts at: 0 1228 1896
Size: 1228 668
SfcI C'TryA_G
Cuts at: 0 1636 1734 1896
Size: 1636 98 162
Fragments arranged by size:
1636 162 98
SfoI GGC'GCC
Cuts at: 0 27 84 1896
Size: 27 57 1812
Fragments arranged by size:
1812 57 27
SmaI CCC'GGG
Cuts at: 0 1415 1896
Size: 1415 481
SmlI C'TyrA_G
Cuts at: 0 308 1042 1896
Size: 308 734 854
Fragments arranged by size:
854 734 308
StuI AGG'CCT
Cuts at: 0 1380 1896
Size: 1380 516
StyI C'CwwG_G
Cuts at: 0 398 474 1160 1500 1613 1869 1896
Size: 398 76 686 340 113 256 27
Fragments arranged by size:
686 398 340 256 113 76 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 616 863 1173 1882 1896
Size: 616 247 310 709 14
Fragments arranged by size:
709 616 310 247 14
TatI w'GTAC_w
Cuts at: 0 1226 1896
Size: 1226 670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1645 1677 1781 1813 1896
Size: 1645 32 104 32 83
Fragments arranged by size:
1645 104 83 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 786 1799 1896
Size: 786 1013 97
Fragments arranged by size:
1013 786 97
XhoI C'TCGA_G
Cuts at: 0 308 1042 1896
Size: 308 734 854
Fragments arranged by size:
854 734 308
XmaI C'CCGG_G
Cuts at: 0 1413 1896
Size: 1413 483
ZraI GAC'GTC
Cuts at: 0 1704 1896
Size: 1704 192
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BclI BglII
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII EaeI EarI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI NspI PacI PasI
PpuMI PspOMI PspXI PsrI PstI PvuI PvuII SacII
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AleI AlfI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BfrBI BglI BlpI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsiWI BsmI BsrBI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI RsrII SacI SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XmnI