Restriction map for
pFN21AE3872
(Linear) (Six-base) MAPSORT of: KIEE3872.seq Check: 6984 from: 1 to: 555
KIEE3872.orf
With 182 enzymes: SgfI *
August 4, 2021 20:19 ..
AfeI AGC'GCT
Cuts at: 0 280 555
Size: 280 275
AlwNI CAG_nnn'CTG
Cuts at: 0 357 555
Size: 357 198
ApaLI G'TGCA_C
Cuts at: 0 342 555
Size: 342 213
AvaI C'yCGr_G
Cuts at: 0 463 474 528 555
Size: 463 11 54 27
Fragments arranged by size:
463 54 27 11
AvrII C'CTAG_G
Cuts at: 0 48 555
Size: 48 507
BanI G'GyrC_C
Cuts at: 0 223 446 555
Size: 223 223 109
Fragments arranged by size:
223 223 109
BanII G_rGCy'C
Cuts at: 0 114 167 519 555
Size: 114 53 352 36
Fragments arranged by size:
352 114 53 36
BbeI G_GCGC'C
Cuts at: 0 450 555
Size: 450 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 485 555
Size: 248 237 70
Fragments arranged by size:
248 237 70
BglI GCCn_nnn'nGGC
Cuts at: 0 431 555
Size: 431 124
BglII A'GATC_T
Cuts at: 0 368 555
Size: 368 187
Bme1580I G_kGCm'C
Cuts at: 0 346 555
Size: 346 209
BmgBI CAC'GTC
Cuts at: 0 174 341 555
Size: 174 167 214
Fragments arranged by size:
214 174 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 555
Size: 503 52
BsaHI Gr'CG_yC
Cuts at: 0 447 555
Size: 447 108
BsaWI w'CCGG_w
Cuts at: 0 252 314 555
Size: 252 62 241
Fragments arranged by size:
252 241 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 555
Size: 370 185
BsiEI CG_ry'CG
Cuts at: 0 17 441 555
Size: 17 424 114
Fragments arranged by size:
424 114 17
BsiHKAI G_wGCw'C
Cuts at: 0 346 543 555
Size: 346 197 12
Fragments arranged by size:
346 197 12
Bsp1286I G_dGCh'C
Cuts at: 0 114 167 346 519 543 555
Size: 114 53 179 173 24 12
Fragments arranged by size:
179 173 114 53 24 12
BspEI T'CCGG_A
Cuts at: 0 252 314 555
Size: 252 62 241
Fragments arranged by size:
252 241 62
BsrBI CCG'CTC
Cuts at: 0 64 555
Size: 64 491
BsrFI r'CCGG_y
Cuts at: 0 443 449 469 555
Size: 443 6 20 86
Fragments arranged by size:
443 86 20 6
BstBI TT'CG_AA
Cuts at: 0 90 555
Size: 90 465
BstYI r'GATC_y
Cuts at: 0 368 555
Size: 368 187
DrdI GACnn_nn'nnGTC
Cuts at: 0 489 555
Size: 489 66
EaeI y'GGCC_r
Cuts at: 0 438 555
Size: 438 117
EagI C'GGCC_G
Cuts at: 0 438 555
Size: 438 117
EarI CTCTTCn'nnn_
Cuts at: 0 121 555
Size: 121 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 555
Size: 503 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 555
Size: 463 92
EcoO109I rG'GnC_Cy
Cuts at: 0 309 555
Size: 309 246
FseI GG_CCGG'CC
Cuts at: 0 473 555
Size: 473 82
HaeII r_GCGC'y
Cuts at: 0 56 282 450 456 555
Size: 56 226 168 6 99
Fragments arranged by size:
226 168 99 56 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 555
Size: 64 32 459
Fragments arranged by size:
459 64 32
KasI G'GCGC_C
Cuts at: 0 446 555
Size: 446 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 62 460 555
Size: 50 12 398 95
Fragments arranged by size:
398 95 50 12
NaeI GCC'GGC
Cuts at: 0 445 451 471 555
Size: 445 6 20 84
Fragments arranged by size:
445 84 20 6
NarI GG'CG_CC
Cuts at: 0 447 555
Size: 447 108
NgoMIV G'CCGG_C
Cuts at: 0 443 449 469 555
Size: 443 6 20 86
Fragments arranged by size:
443 86 20 6
PasI CC'CwG_GG
Cuts at: 0 429 555
Size: 429 126
PshAI GACnn'nnGTC
Cuts at: 0 248 555
Size: 248 307
SfoI GGC'GCC
Cuts at: 0 448 555
Size: 448 107
SgrAI Cr'CCGG_yG
Cuts at: 0 443 449 555
Size: 443 6 106
Fragments arranged by size:
443 106 6
SmaI CCC'GGG
Cuts at: 0 476 555
Size: 476 79
StyI C'CwwG_G
Cuts at: 0 48 555
Size: 48 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 555
Size: 3 552
Tth111I GACn'n_nGTC
Cuts at: 0 400 555
Size: 400 155
XmaI C'CCGG_G
Cuts at: 0 474 555
Size: 474 81
XmnI GAAnn'nnTTC
Cuts at: 0 301 555
Size: 301 254
Enzymes that do cut:
AfeI AlwNI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BglI BglII Bme1580I BmgBI BpmI BsaHI BsaWI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrFI BstBI
BstYI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I KasI MmeI NaeI NarI NgoMIV
PasI PshAI SfoI SgrAI SmaI StyI TaqII Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsiWI BsmI BsmBI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EciI EcoICRI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI ZraI