(Linear) (Six-base) MAPSORT of: KIEE3871.seq  Check: 8816  from: 1  to: 396



KIEE3871.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:19  ..

AleI CACnn'nnGTG

Cuts at:      0     179     396 
   Size:        179     217
 
AvaI C'yCGr_G

Cuts at:      0     103     396 
   Size:        103     293
 
BamHI G'GATC_C

Cuts at:      0      99     396 
   Size:         99     297
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     167     396 
   Size:        167     229
 
BlpI GC'TnA_GC

Cuts at:      0       6     396 
   Size:          6     390
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     396 
   Size:          7     389
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     396 
   Size:          7     389
 
BsrBI CCG'CTC

Cuts at:      0      75     396 
   Size:         75     321
 
BsrFI r'CCGG_y

Cuts at:      0     186     396 
   Size:        186     210
 
BstYI r'GATC_y

Cuts at:      0      99     396 
   Size:         99     297
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85     396 
   Size:         85     311
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     396 
   Size:        171     225
 
MslI CAynn'nnrTG

Cuts at:      0     179     265     396 
   Size:        179      86     131

  Fragments arranged by size: 

                 179     131      86
 
MspA1I CmG'CkG

Cuts at:      0     117     132     396 
   Size:        117      15     264

  Fragments arranged by size: 

                 264     117      15
 
SmaI CCC'GGG

Cuts at:      0     105     396 
   Size:        105     291
 
SpeI A'CTAG_T

Cuts at:      0     354     396 
   Size:        354      42
 
StyI C'CwwG_G

Cuts at:      0     333     348     396 
   Size:        333      15      48

  Fragments arranged by size: 

                 333      48      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106     396 
   Size:        106     290
 
XmaI C'CCGG_G

Cuts at:      0     103     396 
   Size:        103     293
 

 Enzymes that do cut:

     AleI     AvaI    BamHI     BbsI     BlpI  BsiHKAI Bsp1286I    BsrBI
    BsrFI    BstYI     EciI     MmeI     MslI   MspA1I     SmaI     SpeI
     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI