Restriction map for
pFN21AE3811
(Linear) (Six-base) MAPSORT of: KIEE3811.seq Check: 9396 from: 1 to: 1257
KIEE3811.orf
With 182 enzymes: SgfI *
March 22, 2012 16:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 611 1257
Size: 552 59 646
Fragments arranged by size:
646 552 59
AfeI AGC'GCT
Cuts at: 0 110 1257
Size: 110 1147
AlwNI CAG_nnn'CTG
Cuts at: 0 222 385 445 1257
Size: 222 163 60 812
Fragments arranged by size:
812 222 163 60
ApaI G_GGCC'C
Cuts at: 0 25 1257
Size: 25 1232
ApoI r'AATT_y
Cuts at: 0 833 948 1257
Size: 833 115 309
Fragments arranged by size:
833 309 115
BamHI G'GATC_C
Cuts at: 0 873 1257
Size: 873 384
BanI G'GyrC_C
Cuts at: 0 145 225 1257
Size: 145 80 1032
Fragments arranged by size:
1032 145 80
BanII G_rGCy'C
Cuts at: 0 25 137 1112 1257
Size: 25 112 975 145
Fragments arranged by size:
975 145 112 25
BbeI G_GCGC'C
Cuts at: 0 149 229 1257
Size: 149 80 1028
Fragments arranged by size:
1028 149 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 579 1257
Size: 579 678
BclI T'GATC_A
Cuts at: 0 252 783 1208 1257
Size: 252 531 425 49
Fragments arranged by size:
531 425 252 49
Bme1580I G_kGCm'C
Cuts at: 0 25 1191 1257
Size: 25 1166 66
Fragments arranged by size:
1166 66 25
BmtI G_CTAG'C
Cuts at: 0 46 1229 1257
Size: 46 1183 28
Fragments arranged by size:
1183 46 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 526 1257
Size: 467 59 731
Fragments arranged by size:
731 467 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1257
Size: 1081 176
BsaHI Gr'CG_yC
Cuts at: 0 146 226 1257
Size: 146 80 1031
Fragments arranged by size:
1031 146 80
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 1171 1257
Size: 430 741 86
Fragments arranged by size:
741 430 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 321 812 1257
Size: 148 173 491 445
Fragments arranged by size:
491 445 173 148
BsiEI CG_ry'CG
Cuts at: 0 141 171 1257
Size: 141 30 1086
Fragments arranged by size:
1086 141 30
BsiHKAI G_wGCw'C
Cuts at: 0 1112 1257
Size: 1112 145
BsmI GAATG_Cn'
Cuts at: 0 557 973 1239 1257
Size: 557 416 266 18
Fragments arranged by size:
557 416 266 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 703 1257
Size: 703 554
Bsp1286I G_dGCh'C
Cuts at: 0 25 137 1112 1191 1257
Size: 25 112 975 79 66
Fragments arranged by size:
975 112 79 66 25
BspHI T'CATG_A
Cuts at: 0 464 1257
Size: 464 793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 1257
Size: 64 1193
BsrBI CCG'CTC
Cuts at: 0 153 1257
Size: 153 1104
BsrDI GCAATG_nn'
Cuts at: 0 1136 1257
Size: 1136 121
BsrFI r'CCGG_y
Cuts at: 0 69 1039 1257
Size: 69 970 218
Fragments arranged by size:
970 218 69
BsrGI T'GTAC_A
Cuts at: 0 522 1257
Size: 522 735
BstBI TT'CG_AA
Cuts at: 0 1071 1257
Size: 1071 186
BstYI r'GATC_y
Cuts at: 0 873 1082 1257
Size: 873 209 175
Fragments arranged by size:
873 209 175
Bsu36I CC'TnA_GG
Cuts at: 0 1097 1257
Size: 1097 160
BtgI C'CryG_G
Cuts at: 0 141 171 210 240 573 1257
Size: 141 30 39 30 333 684
Fragments arranged by size:
684 333 141 39 30 30
DraI TTT'AAA
Cuts at: 0 345 1257
Size: 345 912
EaeI y'GGCC_r
Cuts at: 0 67 138 213 1257
Size: 67 71 75 1044
Fragments arranged by size:
1044 75 71 67
EagI C'GGCC_G
Cuts at: 0 138 1257
Size: 138 1119
EarI CTCTTCn'nnn_
Cuts at: 0 1097 1257
Size: 1097 160
EcoICRI GAG'CTC
Cuts at: 0 1110 1257
Size: 1110 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 526 552 611 1257
Size: 467 59 26 59 646
Fragments arranged by size:
646 467 59 59 26
EcoO109I rG'GnC_Cy
Cuts at: 0 22 930 1257
Size: 22 908 327
Fragments arranged by size:
908 327 22
EcoRI G'AATT_C
Cuts at: 0 833 1257
Size: 833 424
HaeII r_GCGC'y
Cuts at: 0 112 149 180 229 1257
Size: 112 37 31 49 1028
Fragments arranged by size:
1028 112 49 37 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1010 1042 1074 1106 1257
Size: 340 32 638 32 32 32 151
Fragments arranged by size:
638 340 151 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1011 1257
Size: 1011 246
HpaI GTT'AAC
Cuts at: 0 1011 1257
Size: 1011 246
KasI G'GCGC_C
Cuts at: 0 145 225 1257
Size: 145 80 1032
Fragments arranged by size:
1032 145 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1257
Size: 522 735
MslI CAynn'nnrTG
Cuts at: 0 118 1257
Size: 118 1139
MspA1I CmG'CkG
Cuts at: 0 143 173 212 231 1257
Size: 143 30 39 19 1026
Fragments arranged by size:
1026 143 39 30 19
NaeI GCC'GGC
Cuts at: 0 71 1041 1257
Size: 71 970 216
Fragments arranged by size:
970 216 71
NarI GG'CG_CC
Cuts at: 0 146 226 1257
Size: 146 80 1031
Fragments arranged by size:
1031 146 80
NgoMIV G'CCGG_C
Cuts at: 0 69 1039 1257
Size: 69 970 218
Fragments arranged by size:
970 218 69
NheI G'CTAG_C
Cuts at: 0 42 1225 1257
Size: 42 1183 32
Fragments arranged by size:
1183 42 32
NspI r_CATG'y
Cuts at: 0 853 1257
Size: 853 404
PasI CC'CwG_GG
Cuts at: 0 933 1257
Size: 933 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 445 1257
Size: 222 223 812
Fragments arranged by size:
812 223 222
PspOMI G'GGCC_C
Cuts at: 0 21 1257
Size: 21 1236
PstI C_TGCA'G
Cuts at: 0 61 326 1257
Size: 61 265 931
Fragments arranged by size:
931 265 61
SacI G_AGCT'C
Cuts at: 0 1112 1257
Size: 1112 145
SacII CC_GC'GG
Cuts at: 0 144 174 213 1257
Size: 144 30 39 1044
Fragments arranged by size:
1044 144 39 30
ScaI AGT'ACT
Cuts at: 0 100 1257
Size: 100 1157
SfcI C'TryA_G
Cuts at: 0 57 322 386 1257
Size: 57 265 64 871
Fragments arranged by size:
871 265 64 57
SfoI GGC'GCC
Cuts at: 0 147 227 1257
Size: 147 80 1030
Fragments arranged by size:
1030 147 80
SgrAI Cr'CCGG_yG
Cuts at: 0 1039 1257
Size: 1039 218
SmlI C'TyrA_G
Cuts at: 0 1060 1257
Size: 1060 197
StyI C'CwwG_G
Cuts at: 0 197 1053 1257
Size: 197 856 204
Fragments arranged by size:
856 204 197
TatI w'GTAC_w
Cuts at: 0 98 522 958 1257
Size: 98 424 436 299
Fragments arranged by size:
436 424 299 98
XmnI GAAnn'nnTTC
Cuts at: 0 565 1257
Size: 565 692
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI BamHI BanI BanII
BbeI BbsI BclI Bme1580I BmtI BpmI BpuEI BsaHI
BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I
BtgI DraI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI HaeII Hin4I HincII HpaI KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PflMI
PspOMI PstI SacI SacII ScaI SfcI SfoI SgrAI
SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BplI Bpu10I BsaI BsaAI
BsaBI BsaWI BsaXI BseYI BsiWI BspEI BssHII BssSI
BstAPI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EciI EcoNI EcoRV FalI FseI FspI
FspAI HindIII KpnI MfeI MluI MscI NcoI NdeI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SalI SanDI SapI SbfI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI