Restriction map for
pFN21AE3810
(Linear) (Six-base) MAPSORT of: KIEE3810.seq Check: 6511 from: 1 to: 1221
KIEE3810.orf
With 182 enzymes: SgfI *
March 22, 2012 16:46 ..
AccI GT'mk_AC
Cuts at: 0 716 1118 1221
Size: 716 402 103
Fragments arranged by size:
716 402 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 51 894 1221
Size: 43 8 843 327
Fragments arranged by size:
843 327 43 8
AflIII A'CryG_T
Cuts at: 0 917 1199 1221
Size: 917 282 22
Fragments arranged by size:
917 282 22
AlwNI CAG_nnn'CTG
Cuts at: 0 108 168 1221
Size: 108 60 1053
Fragments arranged by size:
1053 108 60
AvaI C'yCGr_G
Cuts at: 0 307 1221
Size: 307 914
BamHI G'GATC_C
Cuts at: 0 118 303 1221
Size: 118 185 918
Fragments arranged by size:
918 185 118
BanI G'GyrC_C
Cuts at: 0 45 378 949 1136 1221
Size: 45 333 571 187 85
Fragments arranged by size:
571 333 187 85 45
BanII G_rGCy'C
Cuts at: 0 755 989 1193 1221
Size: 755 234 204 28
Fragments arranged by size:
755 234 204 28
BbeI G_GCGC'C
Cuts at: 0 49 1140 1221
Size: 49 1091 81
Fragments arranged by size:
1091 81 49
BbvCI CC'TCA_GC
Cuts at: 0 478 1221
Size: 478 743
BglI GCCn_nnn'nGGC
Cuts at: 0 772 1221
Size: 772 449
BglII A'GATC_T
Cuts at: 0 846 1221
Size: 846 375
BlpI GC'TnA_GC
Cuts at: 0 226 623 1221
Size: 226 397 598
Fragments arranged by size:
598 397 226
Bme1580I G_kGCm'C
Cuts at: 0 383 1181 1221
Size: 383 798 40
Fragments arranged by size:
798 383 40
BmgBI CAC'GTC
Cuts at: 0 375 1202 1221
Size: 375 827 19
Fragments arranged by size:
827 375 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 1221
Size: 159 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1221
Size: 685 536
Bpu10I CC'TnA_GC
Cuts at: 0 325 478 1221
Size: 325 153 743
Fragments arranged by size:
743 325 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 909 1063 1221
Size: 424 485 154 158
Fragments arranged by size:
485 424 158 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 1019 1221
Size: 1019 202
BsaAI yAC'GTr
Cuts at: 0 1208 1221
Size: 1208 13
BsaHI Gr'CG_yC
Cuts at: 0 46 341 1137 1221
Size: 46 295 796 84
Fragments arranged by size:
796 295 84 46
BsaWI w'CCGG_w
Cuts at: 0 356 1221
Size: 356 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 709 739 907 937 1221
Size: 421 30 258 30 168 30 284
Fragments arranged by size:
421 284 258 168 30 30 30
BseYI C'CCAG_C
Cuts at: 0 5 543 941 1180 1221
Size: 5 538 398 239 41
Fragments arranged by size:
538 398 239 41 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 271 1221
Size: 262 9 950
Fragments arranged by size:
950 262 9
BsiEI CG_ry'CG
Cuts at: 0 219 1221
Size: 219 1002
BsiHKAI G_wGCw'C
Cuts at: 0 65 232 755 1221
Size: 65 167 523 466
Fragments arranged by size:
523 466 167 65
BsmI GAATG_Cn'
Cuts at: 0 33 768 1221
Size: 33 735 453
Fragments arranged by size:
735 453 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 441 594 1221
Size: 441 153 627
Fragments arranged by size:
627 441 153
Bsp1286I G_dGCh'C
Cuts at: 0 65 232 383 755 989 1181 1193 1221
Size: 65 167 151 372 234 192 12 28
Fragments arranged by size:
372 234 192 167 151 65 28 12
BspEI T'CCGG_A
Cuts at: 0 356 1221
Size: 356 865
BspHI T'CATG_A
Cuts at: 0 191 1221
Size: 191 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1037 1221
Size: 215 822 184
Fragments arranged by size:
822 215 184
BsrDI GCAATG_nn'
Cuts at: 0 132 1221
Size: 132 1089
BsrFI r'CCGG_y
Cuts at: 0 362 1162 1221
Size: 362 800 59
Fragments arranged by size:
800 362 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 249 1221
Size: 168 81 972
Fragments arranged by size:
972 168 81
BstEII G'GTnAC_C
Cuts at: 0 348 1221
Size: 348 873
BstYI r'GATC_y
Cuts at: 0 118 303 668 846 1221
Size: 118 185 365 178 375
Fragments arranged by size:
375 365 185 178 118
Bsu36I CC'TnA_GG
Cuts at: 0 157 1221
Size: 157 1064
BtgI C'CryG_G
Cuts at: 0 386 1043 1091 1221
Size: 386 657 48 130
Fragments arranged by size:
657 386 130 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 301 440 1221
Size: 301 139 781
Fragments arranged by size:
781 301 139
DrdI GACnn_nn'nnGTC
Cuts at: 0 346 1221
Size: 346 875
EaeI y'GGCC_r
Cuts at: 0 182 1221
Size: 182 1039
EarI CTCTTCn'nnn_
Cuts at: 0 760 913 1042 1221
Size: 760 153 129 179
Fragments arranged by size:
760 179 153 129
EcoICRI GAG'CTC
Cuts at: 0 753 1221
Size: 753 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 51 685 894 1221
Size: 43 8 634 209 327
Fragments arranged by size:
634 327 209 43 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 156 160 298 1221
Size: 156 4 138 923
Fragments arranged by size:
923 156 138 4
EcoO109I rG'GnC_Cy
Cuts at: 0 1083 1221
Size: 1083 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 418 1221
Size: 418 803
HaeII r_GCGC'y
Cuts at: 0 19 49 1110 1140 1221
Size: 19 30 1061 30 81
Fragments arranged by size:
1061 81 30 30 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 421 453 1221
Size: 103 32 286 32 768
Fragments arranged by size:
768 286 103 32 32
KasI G'GCGC_C
Cuts at: 0 45 1136 1221
Size: 45 1091 85
Fragments arranged by size:
1091 85 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1221
Size: 464 757
MscI TGG'CCA
Cuts at: 0 184 1221
Size: 184 1037
MslI CAynn'nnrTG
Cuts at: 0 496 859 1221
Size: 496 363 362
Fragments arranged by size:
496 363 362
MspA1I CmG'CkG
Cuts at: 0 90 246 966 1221
Size: 90 156 720 255
Fragments arranged by size:
720 255 156 90
NaeI GCC'GGC
Cuts at: 0 1164 1221
Size: 1164 57
NarI GG'CG_CC
Cuts at: 0 46 1137 1221
Size: 46 1091 84
Fragments arranged by size:
1091 84 46
NcoI C'CATG_G
Cuts at: 0 386 1043 1221
Size: 386 657 178
Fragments arranged by size:
657 386 178
NgoMIV G'CCGG_C
Cuts at: 0 1162 1221
Size: 1162 59
NspI r_CATG'y
Cuts at: 0 501 744 921 1221
Size: 501 243 177 300
Fragments arranged by size:
501 300 243 177
PciI A'CATG_T
Cuts at: 0 917 1221
Size: 917 304
PfoI T'CCnGG_A
Cuts at: 0 275 293 461 671 1154 1221
Size: 275 18 168 210 483 67
Fragments arranged by size:
483 275 210 168 67 18
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 439 471 1221
Size: 439 32 750
Fragments arranged by size:
750 439 32
PstI C_TGCA'G
Cuts at: 0 1034 1221
Size: 1034 187
PvuII CAG'CTG
Cuts at: 0 246 1221
Size: 246 975
SacI G_AGCT'C
Cuts at: 0 755 1221
Size: 755 466
SapI GCTCTTCn'nnn_
Cuts at: 0 760 1042 1221
Size: 760 282 179
Fragments arranged by size:
760 282 179
ScaI AGT'ACT
Cuts at: 0 457 1015 1221
Size: 457 558 206
Fragments arranged by size:
558 457 206
SfcI C'TryA_G
Cuts at: 0 1030 1221
Size: 1030 191
SfoI GGC'GCC
Cuts at: 0 47 1138 1221
Size: 47 1091 83
Fragments arranged by size:
1091 83 47
SmlI C'TyrA_G
Cuts at: 0 403 888 1078 1221
Size: 403 485 190 143
Fragments arranged by size:
485 403 190 143
SphI G_CATG'C
Cuts at: 0 501 1221
Size: 501 720
StyI C'CwwG_G
Cuts at: 0 386 833 1043 1221
Size: 386 447 210 178
Fragments arranged by size:
447 386 210 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 826 828 884 948 1075 1221
Size: 233 593 2 56 64 127 146
Fragments arranged by size:
593 233 146 127 64 56 2
TatI w'GTAC_w
Cuts at: 0 53 407 455 920 1013 1221
Size: 53 354 48 465 93 208
Fragments arranged by size:
465 354 208 93 53 48
Tth111I GACn'n_nGTC
Cuts at: 0 433 1221
Size: 433 788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 822 1098 1221
Size: 822 276 123
Fragments arranged by size:
822 276 123
Enzymes that do cut:
AccI AcuI AflIII AlwNI AvaI BamHI BanI BanII
BbeI BbvCI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BstAPI BstEII BstYI Bsu36I
BtgI BtgZI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PsrI PstI PvuII SacI SapI ScaI SfcI SfoI
SmlI SphI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BmrI BmtI BsaBI BseRI BsiWI BsrBI BsrGI BssHII
BssSI BstBI BstXI BstZ17I BtsI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI XmnI ZraI