Restriction map for
pFN21AE3808
(Linear) (Six-base) MAPSORT of: KIEE3808.seq Check: 6893 from: 1 to: 1077
KIEE3808.orf
With 182 enzymes: SgfI *
March 22, 2012 16:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1077
Size: 232 845
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1077
Size: 309 768
AvaI C'yCGr_G
Cuts at: 0 871 1077
Size: 871 206
AvrII C'CTAG_G
Cuts at: 0 540 1077
Size: 540 537
BamHI G'GATC_C
Cuts at: 0 867 1077
Size: 867 210
BanI G'GyrC_C
Cuts at: 0 609 1077
Size: 609 468
BanII G_rGCy'C
Cuts at: 0 151 450 623 886 1074 1077
Size: 151 299 173 263 188 3
Fragments arranged by size:
299 263 188 173 151 3
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 1077
Size: 504 573
BbvCI CC'TCA_GC
Cuts at: 0 376 788 797 1077
Size: 376 412 9 280
Fragments arranged by size:
412 376 280 9
BglI GCCn_nnn'nGGC
Cuts at: 0 439 657 1077
Size: 439 218 420
Fragments arranged by size:
439 420 218
BmgBI CAC'GTC
Cuts at: 0 729 1077
Size: 729 348
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 514 930 1077
Size: 429 85 416 147
Fragments arranged by size:
429 416 147 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1014 1077
Size: 1014 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1027 1077
Size: 832 195 50
Fragments arranged by size:
832 195 50
Bpu10I CC'TnA_GC
Cuts at: 0 376 617 788 797 1077
Size: 376 241 171 9 280
Fragments arranged by size:
376 280 241 171 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1077
Size: 984 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 539 1077
Size: 13 526 538
Fragments arranged by size:
538 526 13
BsaWI w'CCGG_w
Cuts at: 0 137 1077
Size: 137 940
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 123 163 1003 1024 1077
Size: 58 65 40 840 21 53
Fragments arranged by size:
840 65 58 53 40 21
BseYI C'CCAG_C
Cuts at: 0 144 179 770 831 1077
Size: 144 35 591 61 246
Fragments arranged by size:
591 246 144 61 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1077
Size: 269 808
BsiEI CG_ry'CG
Cuts at: 0 165 1077
Size: 165 912
BsiHKAI G_wGCw'C
Cuts at: 0 173 450 623 1074 1077
Size: 173 277 173 451 3
Fragments arranged by size:
451 277 173 173 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 285 1077
Size: 285 792
Bsp1286I G_dGCh'C
Cuts at: 0 151 173 450 623 886 1074 1077
Size: 151 22 277 173 263 188 3
Fragments arranged by size:
277 263 188 173 151 22 3
BspEI T'CCGG_A
Cuts at: 0 137 1077
Size: 137 940
BstEII G'GTnAC_C
Cuts at: 0 96 774 1077
Size: 96 678 303
Fragments arranged by size:
678 303 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 812 1077
Size: 812 265
BstYI r'GATC_y
Cuts at: 0 867 1077
Size: 867 210
Bsu36I CC'TnA_GG
Cuts at: 0 26 253 1077
Size: 26 227 824
Fragments arranged by size:
824 227 26
BtgI C'CryG_G
Cuts at: 0 605 665 1077
Size: 605 60 412
Fragments arranged by size:
605 412 60
BtsI GCAGTG_nn'
Cuts at: 0 707 1077
Size: 707 370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1026 1061 1077
Size: 1026 35 16
Fragments arranged by size:
1026 35 16
EaeI y'GGCC_r
Cuts at: 0 162 562 658 1077
Size: 162 400 96 419
Fragments arranged by size:
419 400 162 96
EagI C'GGCC_G
Cuts at: 0 162 1077
Size: 162 915
EarI CTCTTCn'nnn_
Cuts at: 0 700 1077
Size: 700 377
EcoICRI GAG'CTC
Cuts at: 0 448 621 1072 1077
Size: 448 173 451 5
Fragments arranged by size:
451 448 173 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 832 1027 1077
Size: 232 600 195 50
Fragments arranged by size:
600 232 195 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 252 1077
Size: 252 825
EcoO109I rG'GnC_Cy
Cuts at: 0 11 36 316 372 399 678 946 1077
Size: 11 25 280 56 27 279 268 131
Fragments arranged by size:
280 279 268 131 56 27 25 11
HaeII r_GCGC'y
Cuts at: 0 968 1077
Size: 968 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1077
Size: 90 987
MslI CAynn'nnrTG
Cuts at: 0 485 1077
Size: 485 592
MspA1I CmG'CkG
Cuts at: 0 202 275 380 1077
Size: 202 73 105 697
Fragments arranged by size:
697 202 105 73
PasI CC'CwG_GG
Cuts at: 0 812 1077
Size: 812 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 1077
Size: 72 1005
PfoI T'CCnGG_A
Cuts at: 0 899 1077
Size: 899 178
PpuMI rG'GwC_Cy
Cuts at: 0 36 316 372 946 1077
Size: 36 280 56 574 131
Fragments arranged by size:
574 280 131 56 36
PshAI GACnn'nnGTC
Cuts at: 0 551 1077
Size: 551 526
PstI C_TGCA'G
Cuts at: 0 305 392 529 1077
Size: 305 87 137 548
Fragments arranged by size:
548 305 137 87
PvuII CAG'CTG
Cuts at: 0 202 1077
Size: 202 875
SacI G_AGCT'C
Cuts at: 0 450 623 1074 1077
Size: 450 173 451 3
Fragments arranged by size:
451 450 173 3
SanDI GG'GwC_CC
Cuts at: 0 36 1077
Size: 36 1041
SfcI C'TryA_G
Cuts at: 0 301 322 388 525 1077
Size: 301 21 66 137 552
Fragments arranged by size:
552 301 137 66 21
SmlI C'TyrA_G
Cuts at: 0 999 1077
Size: 999 78
StuI AGG'CCT
Cuts at: 0 296 760 1077
Size: 296 464 317
Fragments arranged by size:
464 317 296
StyI C'CwwG_G
Cuts at: 0 540 721 1077
Size: 540 181 356
Fragments arranged by size:
540 356 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 762 1077
Size: 305 457 315
Fragments arranged by size:
457 315 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 881 913 1077
Size: 881 32 164
Fragments arranged by size:
881 164 32
Tth111I GACn'n_nGTC
Cuts at: 0 520 1077
Size: 520 557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 809 1077
Size: 809 268
Enzymes that do cut:
AcuI AlwNI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BglI BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII MmeI MslI MspA1I PasI PflMI PfoI PpuMI
PshAI PstI PvuII SacI SanDI SfcI SmlI StuI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BbeI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmtI BsaAI BsaBI BsaHI
BsaXI BsiWI BsmI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI XmaI XmnI ZraI