Restriction map for
pFN21AE3805
(Linear) (Six-base) MAPSORT of: KIEE3805.seq Check: 3482 from: 1 to: 690
KIEE3805.orf
With 182 enzymes: SgfI *
March 22, 2012 16:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 454 690
Size: 88 366 236
Fragments arranged by size:
366 236 88
AfeI AGC'GCT
Cuts at: 0 338 392 690
Size: 338 54 298
Fragments arranged by size:
338 298 54
ApaI G_GGCC'C
Cuts at: 0 479 690
Size: 479 211
ApoI r'AATT_y
Cuts at: 0 328 690
Size: 328 362
BamHI G'GATC_C
Cuts at: 0 351 690
Size: 351 339
BanII G_rGCy'C
Cuts at: 0 479 690
Size: 479 211
BglII A'GATC_T
Cuts at: 0 285 690
Size: 285 405
BlpI GC'TnA_GC
Cuts at: 0 29 690
Size: 29 661
Bme1580I G_kGCm'C
Cuts at: 0 479 690
Size: 479 211
BmgBI CAC'GTC
Cuts at: 0 111 690
Size: 111 579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 310 690
Size: 209 101 380
Fragments arranged by size:
380 209 101
BsaHI Gr'CG_yC
Cuts at: 0 596 690
Size: 596 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 167 690
Size: 167 523
BseYI C'CCAG_C
Cuts at: 0 98 690
Size: 98 592
BsiEI CG_ry'CG
Cuts at: 0 107 465 690
Size: 107 358 225
Fragments arranged by size:
358 225 107
BsmBI CGTCTCn'nnnn_
Cuts at: 0 550 690
Size: 550 140
Bsp1286I G_dGCh'C
Cuts at: 0 479 690
Size: 479 211
BsrBI CCG'CTC
Cuts at: 0 481 690
Size: 481 209
BsrFI r'CCGG_y
Cuts at: 0 102 690
Size: 102 588
BstYI r'GATC_y
Cuts at: 0 35 156 285 351 449 690
Size: 35 121 129 66 98 241
Fragments arranged by size:
241 129 121 98 66 35
BtgI C'CryG_G
Cuts at: 0 125 191 690
Size: 125 66 499
Fragments arranged by size:
499 125 66
BtsI GCAGTG_nn'
Cuts at: 0 259 425 690
Size: 259 166 265
Fragments arranged by size:
265 259 166
EaeI y'GGCC_r
Cuts at: 0 104 524 690
Size: 104 420 166
Fragments arranged by size:
420 166 104
EagI C'GGCC_G
Cuts at: 0 104 690
Size: 104 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 454 690
Size: 88 366 236
Fragments arranged by size:
366 236 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 690
Size: 319 371
EcoO109I rG'GnC_Cy
Cuts at: 0 416 690
Size: 416 274
HaeII r_GCGC'y
Cuts at: 0 340 394 690
Size: 340 54 296
Fragments arranged by size:
340 296 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 547 579 690
Size: 141 32 374 32 111
Fragments arranged by size:
374 141 111 32 32
MscI TGG'CCA
Cuts at: 0 526 690
Size: 526 164
MspA1I CmG'CkG
Cuts at: 0 127 690
Size: 127 563
NaeI GCC'GGC
Cuts at: 0 104 690
Size: 104 586
NgoMIV G'CCGG_C
Cuts at: 0 102 690
Size: 102 588
NspI r_CATG'y
Cuts at: 0 408 690
Size: 408 282
PpuMI rG'GwC_Cy
Cuts at: 0 416 690
Size: 416 274
PspOMI G'GGCC_C
Cuts at: 0 475 690
Size: 475 215
PvuI CG_AT'CG
Cuts at: 0 465 690
Size: 465 225
SacII CC_GC'GG
Cuts at: 0 128 690
Size: 128 562
SmlI C'TyrA_G
Cuts at: 0 224 289 690
Size: 224 65 401
Fragments arranged by size:
401 224 65
SphI G_CATG'C
Cuts at: 0 408 690
Size: 408 282
StuI AGG'CCT
Cuts at: 0 218 690
Size: 218 472
StyI C'CwwG_G
Cuts at: 0 80 551 690
Size: 80 471 139
Fragments arranged by size:
471 139 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 690
Size: 112 578
XmnI GAAnn'nnTTC
Cuts at: 0 73 690
Size: 73 617
Enzymes that do cut:
AcuI AfeI ApaI ApoI BamHI BanII BglII BlpI
Bme1580I BmgBI BpuEI BsaHI BseRI BseYI BsiEI BsmBI
Bsp1286I BsrBI BsrFI BstYI BtgI BtsI EaeI EagI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MspA1I NaeI
NgoMIV NspI PpuMI PspOMI PvuI SacII SmlI SphI
StuI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI