(Linear) (Six-base) MAPSORT of: KIEE3804.seq  Check: 4527  from: 1  to: 597



KIEE3804.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:45  ..

Acc65I G'GTAC_C

Cuts at:      0     396     521     597 
   Size:        396     125      76

  Fragments arranged by size: 

                 396     125      76
 
AhdI GACnn_n'nnGTC

Cuts at:      0     279     597 
   Size:        279     318
 
AlwNI CAG_nnn'CTG

Cuts at:      0     593     597 
   Size:        593       4
 
ApaLI G'TGCA_C

Cuts at:      0      85     597 
   Size:         85     512
 
BanI G'GyrC_C

Cuts at:      0     396     521     597 
   Size:        396     125      76

  Fragments arranged by size: 

                 396     125      76
 
BanII G_rGCy'C

Cuts at:      0     241     513     597 
   Size:        241     272      84

  Fragments arranged by size: 

                 272     241      84
 
BglI GCCn_nnn'nGGC

Cuts at:      0     508     597 
   Size:        508      89
 
BglII A'GATC_T

Cuts at:      0     179     597 
   Size:        179     418
 
Bme1580I G_kGCm'C

Cuts at:      0      89     597 
   Size:         89     508
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130     597 
   Size:        130     467
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     597 
   Size:        298     299
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     597 
   Size:        398     199
 
BsaWI w'CCGG_w

Cuts at:      0     389     597 
   Size:        389     208
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     223     253     597 
   Size:        223      30     344

  Fragments arranged by size: 

                 344     223      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      89     597 
   Size:         89     508
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     241     513     597 
   Size:         89     152     272      84

  Fragments arranged by size: 

                 272     152      89      84
 
BsrBI CCG'CTC

Cuts at:      0     204     212     597 
   Size:        204       8     385

  Fragments arranged by size: 

                 385     204       8
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     593     597 
   Size:        593       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      54     597 
   Size:         54     543
 
BstYI r'GATC_y

Cuts at:      0     179     597 
   Size:        179     418
 
BtgI C'CryG_G

Cuts at:      0     215     335     353     597 
   Size:        215     120      18     244

  Fragments arranged by size: 

                 244     215     120      18
 
ClaI AT'CG_AT

Cuts at:      0     377     597 
   Size:        377     220
 
EaeI y'GGCC_r

Cuts at:      0     320     500     597 
   Size:        320     180      97

  Fragments arranged by size: 

                 320     180      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     597 
   Size:        298     299
 
EcoRV GAT'ATC

Cuts at:      0     300     597 
   Size:        300     297
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     597 
   Size:        265      32     300

  Fragments arranged by size: 

                 300     265      32
 
KpnI G_GTAC'C

Cuts at:      0     400     525     597 
   Size:        400     125      72

  Fragments arranged by size: 

                 400     125      72
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     597 
   Size:         21     576
 
MscI TGG'CCA

Cuts at:      0     322     502     597 
   Size:        322     180      95

  Fragments arranged by size: 

                 322     180      95
 
MslI CAynn'nnrTG

Cuts at:      0      52      80     475     597 
   Size:         52      28     395     122

  Fragments arranged by size: 

                 395     122      52      28
 
NcoI C'CATG_G

Cuts at:      0     215     335     353     597 
   Size:        215     120      18     244

  Fragments arranged by size: 

                 244     215     120      18
 
SmlI C'TyrA_G

Cuts at:      0     413     597 
   Size:        413     184
 
StyI C'CwwG_G

Cuts at:      0     215     335     353     597 
   Size:        215     120      18     244

  Fragments arranged by size: 

                 244     215     120      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     597 
   Size:        145     452
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     360     597 
   Size:        360     237
 

 Enzymes that do cut:

   Acc65I     AhdI    AlwNI    ApaLI     BanI    BanII     BglI    BglII
 Bme1580I     BmrI     BpmI    BpuEI    BsaWI    BsaXI  BsiHKAI Bsp1286I
    BsrBI   BstAPI    BstXI    BstYI     BtgI     ClaI     EaeI  Eco57MI
    EcoRV    Hin4I     KpnI     MmeI     MscI     MslI     NcoI     SmlI
     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI