(Linear) (Six-base) MAPSORT of: KIEE3803.seq  Check: 4484  from: 1  to: 303



KIEE3803.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:45  ..

Acc65I G'GTAC_C

Cuts at:      0     234     303 
   Size:        234      69
 
AflIII A'CryG_T

Cuts at:      0     180     303 
   Size:        180     123
 
AseI AT'TA_AT

Cuts at:      0     109     303 
   Size:        109     194
 
BanI G'GyrC_C

Cuts at:      0     234     303 
   Size:        234      69
 
BanII G_rGCy'C

Cuts at:      0     231     260     303 
   Size:        231      29      43

  Fragments arranged by size: 

                 231      43      29
 
BlpI GC'TnA_GC

Cuts at:      0     287     303 
   Size:        287      16
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     100     303 
   Size:        100     203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     303 
   Size:         89     214
 
BsiHKAI G_wGCw'C

Cuts at:      0     260     303 
   Size:        260      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     231     260     303 
   Size:        231      29      43

  Fragments arranged by size: 

                 231      43      29
 
EcoICRI GAG'CTC

Cuts at:      0     258     303 
   Size:        258      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     303 
   Size:         89     214
 
FspI TGC'GCA

Cuts at:      0      29     303 
   Size:         29     274
 
KpnI G_GTAC'C

Cuts at:      0     238     303 
   Size:        238      65
 
NspI r_CATG'y

Cuts at:      0     295     303 
   Size:        295       8
 
SacI G_AGCT'C

Cuts at:      0     260     303 
   Size:        260      43
 
SphI G_CATG'C

Cuts at:      0     295     303 
   Size:        295       8
 

 Enzymes that do cut:

   Acc65I   AflIII     AseI     BanI    BanII     BlpI     BplI     BpmI
  BsiHKAI Bsp1286I  EcoICRI  Eco57MI     FspI     KpnI     NspI     SacI
     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI