(Linear) (Six-base) MAPSORT of: KIEE3802.seq  Check: 9643  from: 1  to: 222



KIEE3802.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:45  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     145     222 
   Size:        145      77
 
BanI G'GyrC_C

Cuts at:      0     133     222 
   Size:        133      89
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      68     222 
   Size:         68     154
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     176     206     222 
   Size:        176      30      16

  Fragments arranged by size: 

                 176      30      16
 
BstYI r'GATC_y

Cuts at:      0      29     222 
   Size:         29     193
 
MslI CAynn'nnrTG

Cuts at:      0     200     222 
   Size:        200      22
 

 Enzymes that do cut:

    AlwNI     BanI     BbsI    BsaXI    BstYI     MslI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI