(Linear) (Six-base) MAPSORT of: KIEE3774.seq  Check: 2859  from: 1  to: 657



KIEE3774.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:20  ..

AccI GT'mk_AC

Cuts at:      0     191     657 
   Size:        191     466
 
Acc65I G'GTAC_C

Cuts at:      0     248     657 
   Size:        248     409
 
AflIII A'CryG_T

Cuts at:      0      62     134     610     657 
   Size:         62      72     476      47

  Fragments arranged by size: 

                 476      72      62      47
 
AhdI GACnn_n'nnGTC

Cuts at:      0     558     657 
   Size:        558      99
 
ApoI r'AATT_y

Cuts at:      0      51     657 
   Size:         51     606
 
BanI G'GyrC_C

Cuts at:      0     248     657 
   Size:        248     409
 
BanII G_rGCy'C

Cuts at:      0     598     657 
   Size:        598      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     190     657 
   Size:        190     467
 
BglII A'GATC_T

Cuts at:      0     221     657 
   Size:        221     436
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     354     657 
   Size:        354     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     657 
   Size:         47     610
 
BsaWI w'CCGG_w

Cuts at:      0     251     657 
   Size:        251     406
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     588     657 
   Size:        588      69
 
BseYI C'CCAG_C

Cuts at:      0     144     235     591     657 
   Size:        144      91     356      66

  Fragments arranged by size: 

                 356     144      91      66
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     619     657 
   Size:        619      38
 
Bsp1286I G_dGCh'C

Cuts at:      0     598     657 
   Size:        598      59
 
BsrBI CCG'CTC

Cuts at:      0     246     657 
   Size:        246     411
 
BssSI C'ACGA_G

Cuts at:      0     199     657 
   Size:        199     458
 
BstYI r'GATC_y

Cuts at:      0     221     657 
   Size:        221     436
 
BtgI C'CryG_G

Cuts at:      0     161     276     284     657 
   Size:        161     115       8     373

  Fragments arranged by size: 

                 373     161     115       8
 
BtsI GCAGTG_nn'

Cuts at:      0      99     657 
   Size:         99     558
 
EarI CTCTTCn'nnn_

Cuts at:      0     121     314     416     657 
   Size:        121     193     102     241

  Fragments arranged by size: 

                 241     193     121     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     657 
   Size:         47     610
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     126     657 
   Size:        126     531
 
HaeII r_GCGC'y

Cuts at:      0     534     657 
   Size:        534     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     544     576     657 
   Size:        544      32      81

  Fragments arranged by size: 

                 544      81      32
 
KpnI G_GTAC'C

Cuts at:      0     252     657 
   Size:        252     405
 
MluI A'CGCG_T

Cuts at:      0     610     657 
   Size:        610      47
 
MslI CAynn'nnrTG

Cuts at:      0     298     657 
   Size:        298     359
 
MspA1I CmG'CkG

Cuts at:      0     235     633     657 
   Size:        235     398      24

  Fragments arranged by size: 

                 398     235      24
 
NcoI C'CATG_G

Cuts at:      0     284     657 
   Size:        284     373
 
NspI r_CATG'y

Cuts at:      0      66     657 
   Size:         66     591
 
PciI A'CATG_T

Cuts at:      0      62     657 
   Size:         62     595
 
PstI C_TGCA'G

Cuts at:      0     221     641     657 
   Size:        221     420      16

  Fragments arranged by size: 

                 420     221      16
 
PvuII CAG'CTG

Cuts at:      0     235     657 
   Size:        235     422
 
SfcI C'TryA_G

Cuts at:      0     217     637     657 
   Size:        217     420      20

  Fragments arranged by size: 

                 420     217      20
 
StuI AGG'CCT

Cuts at:      0     523     657 
   Size:        523     134
 
StyI C'CwwG_G

Cuts at:      0     284     657 
   Size:        284     373
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     393     425     657 
   Size:        393      32     232

  Fragments arranged by size: 

                 393     232      32
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     AhdI     ApoI     BanI    BanII     BbsI
    BglII     BmrI     BpmI    BsaWI    BseRI    BseYI    BsmBI Bsp1286I
    BsrBI    BssSI    BstYI     BtgI     BtsI     EarI  Eco57MI     FalI
    HaeII    Hin4I     KpnI     MluI     MslI   MspA1I     NcoI     NspI
     PciI     PstI    PvuII     SfcI     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI