Restriction map for
pFN21AE3767
(Linear) (Six-base) MAPSORT of: KIEE3767.seq Check: 4238 from: 1 to: 2265
KIEE3767.orf
With 182 enzymes: SgfI *
March 22, 2012 16:44 ..
AatII G_ACGT'C
Cuts at: 0 2138 2265
Size: 2138 127
Acc65I G'GTAC_C
Cuts at: 0 2244 2265
Size: 2244 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 457 773 1481 1741 2003 2265
Size: 323 134 316 708 260 262 262
Fragments arranged by size:
708 323 316 262 262 260 134
AflII C'TTAA_G
Cuts at: 0 1920 2265
Size: 1920 345
AflIII A'CryG_T
Cuts at: 0 1010 2265
Size: 1010 1255
AgeI A'CCGG_T
Cuts at: 0 1841 2192 2265
Size: 1841 351 73
Fragments arranged by size:
1841 351 73
AlwNI CAG_nnn'CTG
Cuts at: 0 1459 1719 1821 2229 2265
Size: 1459 260 102 408 36
Fragments arranged by size:
1459 408 260 102 36
ApaI G_GGCC'C
Cuts at: 0 211 2265
Size: 211 2054
ApaLI G'TGCA_C
Cuts at: 0 55 2265
Size: 55 2210
ApoI r'AATT_y
Cuts at: 0 270 637 733 2265
Size: 270 367 96 1532
Fragments arranged by size:
1532 367 270 96
BanI G'GyrC_C
Cuts at: 0 148 2244 2265
Size: 148 2096 21
Fragments arranged by size:
2096 148 21
BanII G_rGCy'C
Cuts at: 0 211 965 1250 1949 2265
Size: 211 754 285 699 316
Fragments arranged by size:
754 699 316 285 211
BbeI G_GCGC'C
Cuts at: 0 152 2265
Size: 152 2113
BbsI GAAGACnn'nnnn_
Cuts at: 0 1183 1918 2182 2265
Size: 1183 735 264 83
Fragments arranged by size:
1183 735 264 83
BclI T'GATC_A
Cuts at: 0 1530 1739 2265
Size: 1530 209 526
Fragments arranged by size:
1530 526 209
BglI GCCn_nnn'nGGC
Cuts at: 0 140 2265
Size: 140 2125
BlpI GC'TnA_GC
Cuts at: 0 1336 2265
Size: 1336 929
Bme1580I G_kGCm'C
Cuts at: 0 59 211 2265
Size: 59 152 2054
Fragments arranged by size:
2054 152 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 1506 2265
Size: 93 1413 759
Fragments arranged by size:
1413 759 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2250 2265
Size: 2250 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1003 1102 1305 1372 2080 2265
Size: 680 323 99 203 67 708 185
Fragments arranged by size:
708 680 323 203 185 99 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 2265
Size: 918 1347
BsaAI yAC'GTr
Cuts at: 0 1011 2265
Size: 1011 1254
BsaBI GATnn'nnATC
Cuts at: 0 286 2265
Size: 286 1979
BsaHI Gr'CG_yC
Cuts at: 0 91 149 2135 2265
Size: 91 58 1986 130
Fragments arranged by size:
1986 130 91 58
BsaWI w'CCGG_w
Cuts at: 0 850 1841 2192 2265
Size: 850 991 351 73
Fragments arranged by size:
991 850 351 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 1114 1144 1828 1858 2265
Size: 803 30 281 30 684 30 407
Fragments arranged by size:
803 684 407 281 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 811 2265
Size: 610 201 1454
Fragments arranged by size:
1454 610 201
BseYI C'CCAG_C
Cuts at: 0 1047 1171 2265
Size: 1047 124 1094
Fragments arranged by size:
1094 1047 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 913 1207 1393 2265
Size: 298 615 294 186 872
Fragments arranged by size:
872 615 298 294 186
BsiHKAI G_wGCw'C
Cuts at: 0 59 965 1250 2265
Size: 59 906 285 1015
Fragments arranged by size:
1015 906 285 59
BsmI GAATG_Cn'
Cuts at: 0 1086 1368 2265
Size: 1086 282 897
Fragments arranged by size:
1086 897 282
Bsp1286I G_dGCh'C
Cuts at: 0 59 211 965 1250 1949 2265
Size: 59 152 754 285 699 316
Fragments arranged by size:
754 699 316 285 152 59
BsrBI CCG'CTC
Cuts at: 0 168 910 2265
Size: 168 742 1355
Fragments arranged by size:
1355 742 168
BsrDI GCAATG_nn'
Cuts at: 0 1562 2265
Size: 1562 703
BsrFI r'CCGG_y
Cuts at: 0 1841 2192 2265
Size: 1841 351 73
Fragments arranged by size:
1841 351 73
BsrGI T'GTAC_A
Cuts at: 0 461 2265
Size: 461 1804
BssSI C'ACGA_G
Cuts at: 0 13 2265
Size: 13 2252
BstEII G'GTnAC_C
Cuts at: 0 1115 2265
Size: 1115 1150
BstYI r'GATC_y
Cuts at: 0 1060 2265
Size: 1060 1205
BtgI C'CryG_G
Cuts at: 0 1307 1757 2265
Size: 1307 450 508
Fragments arranged by size:
1307 508 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 1779 2215 2265
Size: 1248 531 436 50
Fragments arranged by size:
1248 531 436 50
BtsI GCAGTG_nn'
Cuts at: 0 464 615 930 1312 2265
Size: 464 151 315 382 953
Fragments arranged by size:
953 464 382 315 151
ClaI AT'CG_AT
Cuts at: 0 1253 2265
Size: 1253 1012
EaeI y'GGCC_r
Cuts at: 0 2 888 1277 1296 1937 2000 2265
Size: 2 886 389 19 641 63 265
Fragments arranged by size:
886 641 389 265 63 19 2
EarI CTCTTCn'nnn_
Cuts at: 0 242 587 872 1051 2212 2265
Size: 242 345 285 179 1161 53
Fragments arranged by size:
1161 345 285 242 179 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 186 1481 2265
Size: 186 1295 784
Fragments arranged by size:
1295 784 186
EcoICRI GAG'CTC
Cuts at: 0 963 1248 2265
Size: 963 285 1017
Fragments arranged by size:
1017 963 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 457 680 773 1003 1102 1305 1372
Size: 323 134 223 93 230 99 203 67
Cuts at: 1372 1481 1741 2003 2080 2265
Size: 109 260 262 77 185
Fragments arranged by size:
323 262 260 230 223 203 185 134
109 99 93 77 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 973 1831 2265
Size: 202 771 858 434
Fragments arranged by size:
858 771 434 202
EcoO109I rG'GnC_Cy
Cuts at: 0 207 687 2265
Size: 207 480 1578
Fragments arranged by size:
1578 480 207
EcoRV GAT'ATC
Cuts at: 0 1257 2265
Size: 1257 1008
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 2265
Size: 589 1676
FspI TGC'GCA
Cuts at: 0 1889 2265
Size: 1889 376
HaeII r_GCGC'y
Cuts at: 0 152 2265
Size: 152 2113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 586 618 2244 2265
Size: 340 32 214 32 1626 21
Fragments arranged by size:
1626 340 214 32 32 21
HincII GTy'rAC
Cuts at: 0 2112 2265
Size: 2112 153
HindIII A'AGCT_T
Cuts at: 0 433 1684 2265
Size: 433 1251 581
Fragments arranged by size:
1251 581 433
KasI G'GCGC_C
Cuts at: 0 148 2265
Size: 148 2117
KpnI G_GTAC'C
Cuts at: 0 2248 2265
Size: 2248 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1238 2265
Size: 1025 213 1027
Fragments arranged by size:
1027 1025 213
MscI TGG'CCA
Cuts at: 0 4 1939 2002 2265
Size: 4 1935 63 263
Fragments arranged by size:
1935 263 63 4
MslI CAynn'nnrTG
Cuts at: 0 62 1732 2265
Size: 62 1670 533
Fragments arranged by size:
1670 533 62
MspA1I CmG'CkG
Cuts at: 0 186 264 2217 2265
Size: 186 78 1953 48
Fragments arranged by size:
1953 186 78 48
NarI GG'CG_CC
Cuts at: 0 149 2265
Size: 149 2116
NcoI C'CATG_G
Cuts at: 0 1307 2265
Size: 1307 958
PasI CC'CwG_GG
Cuts at: 0 1134 2169 2265
Size: 1134 1035 96
Fragments arranged by size:
1134 1035 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 2169 2265
Size: 2169 96
PfoI T'CCnGG_A
Cuts at: 0 1970 2265
Size: 1970 295
PmlI CAC'GTG
Cuts at: 0 1011 2265
Size: 1011 1254
PshAI GACnn'nnGTC
Cuts at: 0 1979 2265
Size: 1979 286
PspOMI G'GGCC_C
Cuts at: 0 207 2265
Size: 207 2058
PstI C_TGCA'G
Cuts at: 0 206 974 1455 1817 2265
Size: 206 768 481 362 448
Fragments arranged by size:
768 481 448 362 206
PvuII CAG'CTG
Cuts at: 0 186 264 2217 2265
Size: 186 78 1953 48
Fragments arranged by size:
1953 186 78 48
SacI G_AGCT'C
Cuts at: 0 965 1250 2265
Size: 965 285 1015
Fragments arranged by size:
1015 965 285
SapI GCTCTTCn'nnn_
Cuts at: 0 2212 2265
Size: 2212 53
SbfI CC_TGCA'GG
Cuts at: 0 974 1817 2265
Size: 974 843 448
Fragments arranged by size:
974 843 448
ScaI AGT'ACT
Cuts at: 0 481 1852 2265
Size: 481 1371 413
Fragments arranged by size:
1371 481 413
SfcI C'TryA_G
Cuts at: 0 202 426 970 1451 1813 2265
Size: 202 224 544 481 362 452
Fragments arranged by size:
544 481 452 362 224 202
SfoI GGC'GCC
Cuts at: 0 150 2265
Size: 150 2115
SmlI C'TyrA_G
Cuts at: 0 897 1920 2265
Size: 897 1023 345
Fragments arranged by size:
1023 897 345
StuI AGG'CCT
Cuts at: 0 1918 2265
Size: 1918 347
StyI C'CwwG_G
Cuts at: 0 1307 1832 2265
Size: 1307 525 433
Fragments arranged by size:
1307 525 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 931 2265
Size: 75 856 1334
Fragments arranged by size:
1334 856 75
TatI w'GTAC_w
Cuts at: 0 461 479 1104 1769 1850 2265
Size: 461 18 625 665 81 415
Fragments arranged by size:
665 625 461 415 81 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 1512 1544 2181 2213 2265
Size: 803 32 677 32 637 32 52
Fragments arranged by size:
803 677 637 52 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1995 2010 2265
Size: 1995 15 255
Fragments arranged by size:
1995 255 15
XmnI GAAnn'nnTTC
Cuts at: 0 641 1487 2265
Size: 641 846 778
Fragments arranged by size:
846 778 641
ZraI GAC'GTC
Cuts at: 0 2136 2265
Size: 2136 129
Enzymes that do cut:
AatII Acc65I AcuI AflII AflIII AgeI AlwNI ApaI
ApaLI ApoI BanI BanII BbeI BbsI BclI BglI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI
BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI PasI PflMI PfoI PmlI PshAI PspOMI
PstI PvuII SacI SapI SbfI ScaI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AhdI AleI AlfI AloI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BsaI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BssHII BstAPI
BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI
EagI EcoRI FseI FspAI HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI