Restriction map for
pFN21AE3764
(Linear) (Six-base) MAPSORT of: KIEE3764.seq Check: 3713 from: 1 to: 1890
KIEE3764.orf
With 182 enzymes: SgfI *
March 22, 2012 16:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 206 1890
Size: 206 1684
AatII G_ACGT'C
Cuts at: 0 989 1890
Size: 989 901
Acc65I G'GTAC_C
Cuts at: 0 328 1890
Size: 328 1562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 370 748 1748 1890
Size: 227 143 378 1000 142
Fragments arranged by size:
1000 378 227 143 142
AflIII A'CryG_T
Cuts at: 0 1358 1890
Size: 1358 532
AlwNI CAG_nnn'CTG
Cuts at: 0 447 552 1495 1560 1890
Size: 447 105 943 65 330
Fragments arranged by size:
943 447 330 105 65
ApaLI G'TGCA_C
Cuts at: 0 249 1890
Size: 249 1641
ApoI r'AATT_y
Cuts at: 0 1130 1890
Size: 1130 760
AseI AT'TA_AT
Cuts at: 0 1397 1890
Size: 1397 493
AvaI C'yCGr_G
Cuts at: 0 64 688 1890
Size: 64 624 1202
Fragments arranged by size:
1202 624 64
BamHI G'GATC_C
Cuts at: 0 888 1722 1890
Size: 888 834 168
Fragments arranged by size:
888 834 168
BanI G'GyrC_C
Cuts at: 0 59 189 328 339 1485 1507 1890
Size: 59 130 139 11 1146 22 383
Fragments arranged by size:
1146 383 139 130 59 22 11
BanII G_rGCy'C
Cuts at: 0 163 238 1468 1890
Size: 163 75 1230 422
Fragments arranged by size:
1230 422 163 75
BglI GCCn_nnn'nGGC
Cuts at: 0 292 1890
Size: 292 1598
BlpI GC'TnA_GC
Cuts at: 0 1591 1890
Size: 1591 299
Bme1580I G_kGCm'C
Cuts at: 0 62 253 490 1890
Size: 62 191 237 1400
Fragments arranged by size:
1400 237 191 62
BmgBI CAC'GTC
Cuts at: 0 116 1890
Size: 116 1774
BmrI ACTGGGnnnn_n'
Cuts at: 0 1468 1890
Size: 1468 422
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1390 1890
Size: 1390 500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 707 902 1890
Size: 340 367 195 988
Fragments arranged by size:
988 367 340 195
BsaAI yAC'GTr
Cuts at: 0 918 1361 1890
Size: 918 443 529
Fragments arranged by size:
918 529 443
BsaBI GATnn'nnATC
Cuts at: 0 1862 1890
Size: 1862 28
BsaHI Gr'CG_yC
Cuts at: 0 986 1890
Size: 986 904
BsaWI w'CCGG_w
Cuts at: 0 136 1019 1890
Size: 136 883 871
Fragments arranged by size:
883 871 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 131 161 414 444 1697 1727 1890
Size: 131 30 253 30 1253 30 163
Fragments arranged by size:
1253 253 163 131 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1087 1114 1806 1890
Size: 1087 27 692 84
Fragments arranged by size:
1087 692 84 27
BseYI C'CCAG_C
Cuts at: 0 1331 1517 1890
Size: 1331 186 373
Fragments arranged by size:
1331 373 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 410 1278 1345 1890
Size: 58 352 868 67 545
Fragments arranged by size:
868 545 352 67 58
BsiEI CG_ry'CG
Cuts at: 0 149 1890
Size: 149 1741
BsiHKAI G_wGCw'C
Cuts at: 0 253 1890
Size: 253 1637
BsmI GAATG_Cn'
Cuts at: 0 967 1579 1878 1890
Size: 967 612 299 12
Fragments arranged by size:
967 612 299 12
Bsp1286I G_dGCh'C
Cuts at: 0 62 163 238 253 490 1468 1890
Size: 62 101 75 15 237 978 422
Fragments arranged by size:
978 422 237 101 75 62 15
BspEI T'CCGG_A
Cuts at: 0 136 1890
Size: 136 1754
BspHI T'CATG_A
Cuts at: 0 1113 1890
Size: 1113 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 1890
Size: 206 1684
BssSI C'ACGA_G
Cuts at: 0 34 1890
Size: 34 1856
BstYI r'GATC_y
Cuts at: 0 888 927 1371 1722 1890
Size: 888 39 444 351 168
Fragments arranged by size:
888 444 351 168 39
BtgI C'CryG_G
Cuts at: 0 1190 1468 1890
Size: 1190 278 422
Fragments arranged by size:
1190 422 278
BtsI GCAGTG_nn'
Cuts at: 0 1269 1473 1890
Size: 1269 204 417
Fragments arranged by size:
1269 417 204
EaeI y'GGCC_r
Cuts at: 0 12 284 293 1665 1770 1890
Size: 12 272 9 1372 105 120
Fragments arranged by size:
1372 272 120 105 12 9
EarI CTCTTCn'nnn_
Cuts at: 0 58 396 1415 1890
Size: 58 338 1019 475
Fragments arranged by size:
1019 475 338 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 340 370 707 748 902 1748 1890
Size: 227 113 30 337 41 154 846 142
Fragments arranged by size:
846 337 227 154 142 113 41 30
EcoO109I rG'GnC_Cy
Cuts at: 0 369 444 1890
Size: 369 75 1446
Fragments arranged by size:
1446 369 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1852 1890
Size: 1852 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 414 446 1095 1127 1734 1766
Size: 131 32 251 32 649 32 607 32
Cuts at: 1766 1890
Size: 124
Fragments arranged by size:
649 607 251 131 124 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 332 1890
Size: 332 1558
MscI TGG'CCA
Cuts at: 0 286 1667 1890
Size: 286 1381 223
Fragments arranged by size:
1381 286 223
MslI CAynn'nnrTG
Cuts at: 0 817 1890
Size: 817 1073
MspA1I CmG'CkG
Cuts at: 0 383 1560 1890
Size: 383 1177 330
Fragments arranged by size:
1177 383 330
NcoI C'CATG_G
Cuts at: 0 1468 1890
Size: 1468 422
NdeI CA'TA_TG
Cuts at: 0 857 1790 1890
Size: 857 933 100
Fragments arranged by size:
933 857 100
NspI r_CATG'y
Cuts at: 0 806 1890
Size: 806 1084
PasI CC'CwG_GG
Cuts at: 0 502 1890
Size: 502 1388
PflMI CCAn_nnn'nTGG
Cuts at: 0 1156 1458 1890
Size: 1156 302 432
Fragments arranged by size:
1156 432 302
PfoI T'CCnGG_A
Cuts at: 0 356 418 1890
Size: 356 62 1472
Fragments arranged by size:
1472 356 62
PmlI CAC'GTG
Cuts at: 0 1361 1890
Size: 1361 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 1890
Size: 145 32 1713
Fragments arranged by size:
1713 145 32
PpuMI rG'GwC_Cy
Cuts at: 0 369 444 1890
Size: 369 75 1446
Fragments arranged by size:
1446 369 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 633 665 1890
Size: 633 32 1225
Fragments arranged by size:
1225 633 32
PvuII CAG'CTG
Cuts at: 0 1560 1890
Size: 1560 330
SanDI GG'GwC_CC
Cuts at: 0 369 1890
Size: 369 1521
SapI GCTCTTCn'nnn_
Cuts at: 0 1415 1890
Size: 1415 475
ScaI AGT'ACT
Cuts at: 0 1033 1890
Size: 1033 857
SexAI A'CCwGG_T
Cuts at: 0 446 1890
Size: 446 1444
SfcI C'TryA_G
Cuts at: 0 544 899 1687 1890
Size: 544 355 788 203
Fragments arranged by size:
788 544 355 203
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 292 1890
Size: 292 1598
SmaI CCC'GGG
Cuts at: 0 66 690 1890
Size: 66 624 1200
Fragments arranged by size:
1200 624 66
SnaBI TAC'GTA
Cuts at: 0 918 1890
Size: 918 972
SspI AAT'ATT
Cuts at: 0 1120 1890
Size: 1120 770
StyI C'CwwG_G
Cuts at: 0 119 747 1468 1745 1890
Size: 119 628 721 277 145
Fragments arranged by size:
721 628 277 145 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 1890
Size: 135 1755
TatI w'GTAC_w
Cuts at: 0 86 1031 1417 1890
Size: 86 945 386 473
Fragments arranged by size:
945 473 386 86
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1197 1890
Size: 1197 693
XmaI C'CCGG_G
Cuts at: 0 64 688 1890
Size: 64 624 1202
Fragments arranged by size:
1202 624 64
XmnI GAAnn'nnTTC
Cuts at: 0 1496 1890
Size: 1496 394
ZraI GAC'GTC
Cuts at: 0 987 1890
Size: 987 903
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI ApaLI ApoI
AseI AvaI BamHI BanI BanII BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BssSI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoO109I FalI Hin4I KpnI MscI MslI
MspA1I NcoI NdeI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PsrI PvuII SanDI SapI ScaI SexAI
SfcI SfiI SmaI SnaBI SspI StyI TaqII TatI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI Bpu10I
BpuEI BsaI BsiWI BsmBI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SbfI SfoI SgfI SgrAI SmlI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI