Restriction map for
pFN21AE3763
(Linear) (Six-base) MAPSORT of: KIEE3763.seq Check: 7786 from: 1 to: 1677
KIEE3763.orf
With 182 enzymes: SgfI *
March 22, 2012 16:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 771 1677
Size: 771 906
AccI GT'mk_AC
Cuts at: 0 1245 1377 1677
Size: 1245 132 300
Fragments arranged by size:
1245 300 132
AleI CACnn'nnGTG
Cuts at: 0 1199 1677
Size: 1199 478
AlwNI CAG_nnn'CTG
Cuts at: 0 17 1462 1528 1677
Size: 17 1445 66 149
Fragments arranged by size:
1445 149 66 17
ApoI r'AATT_y
Cuts at: 0 224 535 1633 1664 1677
Size: 224 311 1098 31 13
Fragments arranged by size:
1098 311 224 31 13
BamHI G'GATC_C
Cuts at: 0 786 870 984 1677
Size: 786 84 114 693
Fragments arranged by size:
786 693 114 84
BanI G'GyrC_C
Cuts at: 0 106 1677
Size: 106 1571
BbvCI CC'TCA_GC
Cuts at: 0 1529 1677
Size: 1529 148
BclI T'GATC_A
Cuts at: 0 1089 1677
Size: 1089 588
Bme1580I G_kGCm'C
Cuts at: 0 1155 1677
Size: 1155 522
BmrI ACTGGGnnnn_n'
Cuts at: 0 155 1677
Size: 155 1522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1677
Size: 39 1638
Bpu10I CC'TnA_GC
Cuts at: 0 1529 1677
Size: 1529 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1520 1677
Size: 220 1300 157
Fragments arranged by size:
1300 220 157
BsaBI GATnn'nnATC
Cuts at: 0 1091 1482 1677
Size: 1091 391 195
Fragments arranged by size:
1091 391 195
BsaHI Gr'CG_yC
Cuts at: 0 384 1677
Size: 384 1293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1452 1482 1677
Size: 1452 30 195
Fragments arranged by size:
1452 195 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 1677
Size: 341 1336
BseYI C'CCAG_C
Cuts at: 0 96 1677
Size: 96 1581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1677
Size: 557 1120
BsiHKAI G_wGCw'C
Cuts at: 0 940 1677
Size: 940 737
BsmI GAATG_Cn'
Cuts at: 0 492 1677
Size: 492 1185
Bsp1286I G_dGCh'C
Cuts at: 0 940 1155 1677
Size: 940 215 522
Fragments arranged by size:
940 522 215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 771 1677
Size: 771 906
BstYI r'GATC_y
Cuts at: 0 398 719 786 870 984 1677
Size: 398 321 67 84 114 693
Fragments arranged by size:
693 398 321 114 84 67
Bsu36I CC'TnA_GG
Cuts at: 0 866 1677
Size: 866 811
BtsI GCAGTG_nn'
Cuts at: 0 636 1677
Size: 636 1041
ClaI AT'CG_AT
Cuts at: 0 1500 1677
Size: 1500 177
DraI TTT'AAA
Cuts at: 0 743 1677
Size: 743 934
DrdI GACnn_nn'nnGTC
Cuts at: 0 1619 1677
Size: 1619 58
EaeI y'GGCC_r
Cuts at: 0 72 1677
Size: 72 1605
EarI CTCTTCn'nnn_
Cuts at: 0 790 938 1593 1677
Size: 790 148 655 84
Fragments arranged by size:
790 655 148 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1677
Size: 39 1638
EcoO109I rG'GnC_Cy
Cuts at: 0 843 1677
Size: 843 834
EcoRV GAT'ATC
Cuts at: 0 816 1677
Size: 816 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1576 1677
Size: 1576 101
FspI TGC'GCA
Cuts at: 0 4 1677
Size: 4 1673
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 859 891 1452 1484 1677
Size: 159 32 668 32 561 32 193
Fragments arranged by size:
668 561 193 159 32 32 32
HincII GTy'rAC
Cuts at: 0 1246 1599 1677
Size: 1246 353 78
Fragments arranged by size:
1246 353 78
HindIII A'AGCT_T
Cuts at: 0 238 1677
Size: 238 1439
MfeI C'AATT_G
Cuts at: 0 1288 1677
Size: 1288 389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 1677
Size: 417 1260
MslI CAynn'nnrTG
Cuts at: 0 307 485 1199 1677
Size: 307 178 714 478
Fragments arranged by size:
714 478 307 178
MspA1I CmG'CkG
Cuts at: 0 959 1677
Size: 959 718
NdeI CA'TA_TG
Cuts at: 0 431 773 1677
Size: 431 342 904
Fragments arranged by size:
904 431 342
PflMI CCAn_nnn'nTGG
Cuts at: 0 1615 1677
Size: 1615 62
PfoI T'CCnGG_A
Cuts at: 0 16 1677
Size: 16 1661
PpuMI rG'GwC_Cy
Cuts at: 0 843 1677
Size: 843 834
PstI C_TGCA'G
Cuts at: 0 782 1677
Size: 782 895
PvuII CAG'CTG
Cuts at: 0 959 1677
Size: 959 718
SalI G'TCGA_C
Cuts at: 0 1244 1677
Size: 1244 433
SexAI A'CCwGG_T
Cuts at: 0 272 1677
Size: 272 1405
SfcI C'TryA_G
Cuts at: 0 778 1378 1677
Size: 778 600 299
Fragments arranged by size:
778 600 299
SmlI C'TyrA_G
Cuts at: 0 235 1535 1677
Size: 235 1300 142
Fragments arranged by size:
1300 235 142
SspI AAT'ATT
Cuts at: 0 739 1677
Size: 739 938
StyI C'CwwG_G
Cuts at: 0 1147 1197 1677
Size: 1147 50 480
Fragments arranged by size:
1147 480 50
SwaI ATTT'AAAT
Cuts at: 0 743 1677
Size: 743 934
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 576 1677
Size: 76 500 1101
Fragments arranged by size:
1101 500 76
TatI w'GTAC_w
Cuts at: 0 283 1677
Size: 283 1394
Enzymes that do cut:
AarI AccI AleI AlwNI ApoI BamHI BanI BbvCI
BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BstYI Bsu36I BtsI ClaI DraI DrdI EaeI EarI
Eco57MI EcoO109I EcoRV FalI FspI Hin4I HincII HindIII
MfeI MmeI MslI MspA1I NdeI PflMI PfoI PpuMI
PstI PvuII SalI SexAI SfcI SmlI SspI StyI
SwaI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbsI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI
BsaAI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI CspCI DraIII EagI EciI
EcoICRI EcoNI EcoRI FseI FspAI HaeII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI