Restriction map for
pFN21AE3760
(Linear) (Six-base) MAPSORT of: KIEE3760.seq Check: 4415 from: 1 to: 1482
KIEE3760.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 416 1482
Size: 416 1066
AccI GT'mk_AC
Cuts at: 0 4 35 1482
Size: 4 31 1447
Fragments arranged by size:
1447 31 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1269 1440 1482
Size: 1269 171 42
Fragments arranged by size:
1269 171 42
AflII C'TTAA_G
Cuts at: 0 301 1482
Size: 301 1181
AhdI GACnn_n'nnGTC
Cuts at: 0 1356 1482
Size: 1356 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 1482
Size: 349 1133
AlwNI CAG_nnn'CTG
Cuts at: 0 257 1206 1482
Size: 257 949 276
Fragments arranged by size:
949 276 257
ApaI G_GGCC'C
Cuts at: 0 1044 1482
Size: 1044 438
ApoI r'AATT_y
Cuts at: 0 352 1482
Size: 352 1130
AvaI C'yCGr_G
Cuts at: 0 135 234 780 810 820 945 1482
Size: 135 99 546 30 10 125 537
Fragments arranged by size:
546 537 135 125 99 30 10
BanI G'GyrC_C
Cuts at: 0 238 295 682 949 1006 1015 1032 1209
Size: 238 57 387 267 57 9 17 177
Cuts at: 1209 1230 1482
Size: 21 252
Fragments arranged by size:
387 267 252 238 177 57 57 21
17 9
BanII G_rGCy'C
Cuts at: 0 1044 1106 1396 1482
Size: 1044 62 290 86
Fragments arranged by size:
1044 290 86 62
BbeI G_GCGC'C
Cuts at: 0 242 686 953 1019 1036 1213 1234 1482
Size: 242 444 267 66 17 177 21 248
Fragments arranged by size:
444 267 248 242 177 66 21 17
BbvCI CC'TCA_GC
Cuts at: 0 772 1405 1482
Size: 772 633 77
Fragments arranged by size:
772 633 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1256 1290 1482
Size: 1256 34 192
Fragments arranged by size:
1256 192 34
BglI GCCn_nnn'nGGC
Cuts at: 0 931 973 1156 1409 1482
Size: 931 42 183 253 73
Fragments arranged by size:
931 253 183 73 42
BlpI GC'TnA_GC
Cuts at: 0 1105 1482
Size: 1105 377
Bme1580I G_kGCm'C
Cuts at: 0 1044 1482
Size: 1044 438
BmgBI CAC'GTC
Cuts at: 0 851 1482
Size: 851 631
Bpu10I CC'TnA_GC
Cuts at: 0 772 1378 1390 1405 1482
Size: 772 606 12 15 77
Fragments arranged by size:
772 606 77 15 12
BsaAI yAC'GTr
Cuts at: 0 500 1482
Size: 500 982
BsaHI Gr'CG_yC
Cuts at: 0 112 239 683 950 1016 1033 1210 1231
Size: 112 127 444 267 66 17 177 21
Cuts at: 1231 1482
Size: 251
Fragments arranged by size:
444 267 251 177 127 112 66 21
17
BsaWI w'CCGG_w
Cuts at: 0 1098 1482
Size: 1098 384
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 888 891 900 1482
Size: 502 386 3 9 582
Fragments arranged by size:
582 502 386 9 3
BseYI C'CCAG_C
Cuts at: 0 130 205 1237 1339 1423 1482
Size: 130 75 1032 102 84 59
Fragments arranged by size:
1032 130 102 84 75 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 745 1482
Size: 388 357 737
Fragments arranged by size:
737 388 357
BsiEI CG_ry'CG
Cuts at: 0 275 387 980 992 1482
Size: 275 112 593 12 490
Fragments arranged by size:
593 490 275 112 12
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1396 1482
Size: 1106 290 86
Fragments arranged by size:
1106 290 86
Bsp1286I G_dGCh'C
Cuts at: 0 1044 1106 1396 1482
Size: 1044 62 290 86
Fragments arranged by size:
1044 290 86 62
BspEI T'CCGG_A
Cuts at: 0 1098 1482
Size: 1098 384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 416 704 1034 1482
Size: 188 228 288 330 448
Fragments arranged by size:
448 330 288 228 188
BsrBI CCG'CTC
Cuts at: 0 365 494 660 994 1125 1482
Size: 365 129 166 334 131 357
Fragments arranged by size:
365 357 334 166 131 129
BsrFI r'CCGG_y
Cuts at: 0 274 476 1227 1482
Size: 274 202 751 255
Fragments arranged by size:
751 274 255 202
BssSI C'ACGA_G
Cuts at: 0 541 1068 1482
Size: 541 527 414
Fragments arranged by size:
541 527 414
BstAPI GCAn_nnn'nTGC
Cuts at: 0 257 402 1482
Size: 257 145 1080
Fragments arranged by size:
1080 257 145
BtgI C'CryG_G
Cuts at: 0 739 748 929 971 980 1154 1482
Size: 739 9 181 42 9 174 328
Fragments arranged by size:
739 328 181 174 42 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 731 1482
Size: 731 751
EaeI y'GGCC_r
Cuts at: 0 65 119 170 272 977 989 1157 1437
Size: 65 54 51 102 705 12 168 280
Cuts at: 1437 1482
Size: 45
Fragments arranged by size:
705 280 168 102 65 54 51 45
12
EagI C'GGCC_G
Cuts at: 0 272 977 989 1482
Size: 272 705 12 493
Fragments arranged by size:
705 493 272 12
EarI CTCTTCn'nnn_
Cuts at: 0 885 1482
Size: 885 597
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 9 894 1482
Size: 9 885 588
Fragments arranged by size:
885 588 9
EcoICRI GAG'CTC
Cuts at: 0 1104 1394 1482
Size: 1104 290 88
Fragments arranged by size:
1104 290 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1269 1440 1482
Size: 1269 171 42
Fragments arranged by size:
1269 171 42
EcoO109I rG'GnC_Cy
Cuts at: 0 693 1023 1040 1041 1203 1482
Size: 693 330 17 1 162 279
Fragments arranged by size:
693 330 279 162 17 1
FspI TGC'GCA
Cuts at: 0 550 1482
Size: 550 932
HaeII r_GCGC'y
Cuts at: 0 242 686 953 1019 1036 1213 1234 1482
Size: 242 444 267 66 17 177 21 248
Fragments arranged by size:
444 267 248 242 177 66 21 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 1342 1374 1482
Size: 549 32 761 32 108
Fragments arranged by size:
761 549 108 32 32
HincII GTy'rAC
Cuts at: 0 5 36 1482
Size: 5 31 1446
Fragments arranged by size:
1446 31 5
KasI G'GCGC_C
Cuts at: 0 238 682 949 1015 1032 1209 1230 1482
Size: 238 444 267 66 17 177 21 252
Fragments arranged by size:
444 267 252 238 177 66 21 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 307 1337 1340 1482
Size: 183 124 1030 3 142
Fragments arranged by size:
1030 183 142 124 3
MscI TGG'CCA
Cuts at: 0 67 1482
Size: 67 1415
MspA1I CmG'CkG
Cuts at: 0 218 257 741 750 831 931 973 982
Size: 218 39 484 9 81 100 42 9
Cuts at: 982 1296 1409 1482
Size: 314 113 73
Fragments arranged by size:
484 314 218 113 100 81 73 42
39 9 9
NaeI GCC'GGC
Cuts at: 0 276 478 1229 1482
Size: 276 202 751 253
Fragments arranged by size:
751 276 253 202
NarI GG'CG_CC
Cuts at: 0 239 683 950 1016 1033 1210 1231 1482
Size: 239 444 267 66 17 177 21 251
Fragments arranged by size:
444 267 251 239 177 66 21 17
NgoMIV G'CCGG_C
Cuts at: 0 274 476 1227 1482
Size: 274 202 751 255
Fragments arranged by size:
751 274 255 202
NotI GC'GGCC_GC
Cuts at: 0 977 989 1482
Size: 977 12 493
Fragments arranged by size:
977 493 12
NspI r_CATG'y
Cuts at: 0 90 187 962 1482
Size: 90 97 775 520
Fragments arranged by size:
775 520 97 90
PfoI T'CCnGG_A
Cuts at: 0 356 1482
Size: 356 1126
PmlI CAC'GTG
Cuts at: 0 500 1482
Size: 500 982
PpuMI rG'GwC_Cy
Cuts at: 0 693 1023 1482
Size: 693 330 459
Fragments arranged by size:
693 459 330
PspOMI G'GGCC_C
Cuts at: 0 1040 1482
Size: 1040 442
PspXI vC'TCGA_Gb
Cuts at: 0 820 1482
Size: 820 662
PstI C_TGCA'G
Cuts at: 0 413 1482
Size: 413 1069
PvuI CG_AT'CG
Cuts at: 0 387 1482
Size: 387 1095
PvuII CAG'CTG
Cuts at: 0 218 257 1296 1482
Size: 218 39 1039 186
Fragments arranged by size:
1039 218 186 39
RsrII CG'GwC_CG
Cuts at: 0 280 1096 1362 1482
Size: 280 816 266 120
Fragments arranged by size:
816 280 266 120
SacI G_AGCT'C
Cuts at: 0 1106 1396 1482
Size: 1106 290 86
Fragments arranged by size:
1106 290 86
SacII CC_GC'GG
Cuts at: 0 742 751 932 974 983 1482
Size: 742 9 181 42 9 499
Fragments arranged by size:
742 499 181 42 9 9
SalI G'TCGA_C
Cuts at: 0 3 34 1482
Size: 3 31 1448
Fragments arranged by size:
1448 31 3
SapI GCTCTTCn'nnn_
Cuts at: 0 885 1482
Size: 885 597
SfcI C'TryA_G
Cuts at: 0 409 1482
Size: 409 1073
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 931 1482
Size: 931 551
SfoI GGC'GCC
Cuts at: 0 240 684 951 1017 1034 1211 1232 1482
Size: 240 444 267 66 17 177 21 250
Fragments arranged by size:
444 267 250 240 177 66 21 17
SgrAI Cr'CCGG_yG
Cuts at: 0 1227 1482
Size: 1227 255
SmaI CCC'GGG
Cuts at: 0 236 812 1482
Size: 236 576 670
Fragments arranged by size:
670 576 236
SmlI C'TyrA_G
Cuts at: 0 301 820 1482
Size: 301 519 662
Fragments arranged by size:
662 519 301
SphI G_CATG'C
Cuts at: 0 187 1482
Size: 187 1295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 595 1084 1482
Size: 481 114 489 398
Fragments arranged by size:
489 481 398 114
TatI w'GTAC_w
Cuts at: 0 572 1482
Size: 572 910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 529 1482
Size: 529 953
XhoI C'TCGA_G
Cuts at: 0 820 1482
Size: 820 662
XmaI C'CCGG_G
Cuts at: 0 234 810 1482
Size: 234 576 672
Fragments arranged by size:
672 576 234
XmnI GAAnn'nnTTC
Cuts at: 0 352 1482
Size: 352 1130
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmgBI Bpu10I BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BssSI BstAPI BtgI BtgZI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII
KasI MmeI MscI MspA1I NaeI NarI NgoMIV NotI
NspI PfoI PmlI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI TaqII TatI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BciVI BclI BfrBI BglII BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI MslI NcoI NdeI NheI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
ZraI