Restriction map for
pFN21AE3758
(Linear) (Six-base) MAPSORT of: KIEE3758.seq Check: 2347 from: 1 to: 1293
KIEE3758.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 312 635 1293
Size: 19 293 323 658
Fragments arranged by size:
658 323 293 19
AgeI A'CCGG_T
Cuts at: 0 597 1293
Size: 597 696
AleI CACnn'nnGTG
Cuts at: 0 991 1293
Size: 991 302
AlwNI CAG_nnn'CTG
Cuts at: 0 42 1293
Size: 42 1251
ApoI r'AATT_y
Cuts at: 0 568 882 1293
Size: 568 314 411
Fragments arranged by size:
568 411 314
BamHI G'GATC_C
Cuts at: 0 279 1293
Size: 279 1014
BanI G'GyrC_C
Cuts at: 0 394 981 1293
Size: 394 587 312
Fragments arranged by size:
587 394 312
BanII G_rGCy'C
Cuts at: 0 186 551 1036 1293
Size: 186 365 485 257
Fragments arranged by size:
485 365 257 186
BbvCI CC'TCA_GC
Cuts at: 0 386 1293
Size: 386 907
BciVI GTATCCnnnnn_n'
Cuts at: 0 1129 1293
Size: 1129 164
BclI T'GATC_A
Cuts at: 0 995 1293
Size: 995 298
BmrI ACTGGGnnnn_n'
Cuts at: 0 1030 1293
Size: 1030 263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1293
Size: 1055 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1098 1149 1293
Size: 913 185 51 144
Fragments arranged by size:
913 185 144 51
Bpu10I CC'TnA_GC
Cuts at: 0 386 1293
Size: 386 907
BsaWI w'CCGG_w
Cuts at: 0 597 1293
Size: 597 696
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 459 530 1293
Size: 459 71 763
Fragments arranged by size:
763 459 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 1293
Size: 133 1160
Bsp1286I G_dGCh'C
Cuts at: 0 186 551 1036 1293
Size: 186 365 485 257
Fragments arranged by size:
485 365 257 186
BsrDI GCAATG_nn'
Cuts at: 0 420 1293
Size: 420 873
BsrFI r'CCGG_y
Cuts at: 0 597 1293
Size: 597 696
BsrGI T'GTAC_A
Cuts at: 0 242 1293
Size: 242 1051
BssSI C'ACGA_G
Cuts at: 0 999 1293
Size: 999 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 1293
Size: 357 936
BstYI r'GATC_y
Cuts at: 0 279 952 1293
Size: 279 673 341
Fragments arranged by size:
673 341 279
Bsu36I CC'TnA_GG
Cuts at: 0 542 1293
Size: 542 751
BtsI GCAGTG_nn'
Cuts at: 0 1011 1293
Size: 1011 282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 272 307 1293
Size: 272 35 986
Fragments arranged by size:
986 272 35
DraIII CAC_nnn'GTG
Cuts at: 0 20 1293
Size: 20 1273
EarI CTCTTCn'nnn_
Cuts at: 0 110 1293
Size: 110 1183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 312 635 913 1098 1149 1293
Size: 19 293 323 278 185 51 144
Fragments arranged by size:
323 293 278 185 144 51 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 520 524 552 871 903 993 1025
Size: 492 28 4 28 319 32 90 32
Cuts at: 1025 1293
Size: 268
Fragments arranged by size:
492 319 268 90 32 32 28 28
4
HindIII A'AGCT_T
Cuts at: 0 100 481 668 1293
Size: 100 381 187 625
Fragments arranged by size:
625 381 187 100
MfeI C'AATT_G
Cuts at: 0 626 1293
Size: 626 667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1293
Size: 55 1238
MslI CAynn'nnrTG
Cuts at: 0 991 1293
Size: 991 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 1293
Size: 672 621
PstI C_TGCA'G
Cuts at: 0 47 1293
Size: 47 1246
ScaI AGT'ACT
Cuts at: 0 1134 1293
Size: 1134 159
SfcI C'TryA_G
Cuts at: 0 43 1046 1282 1293
Size: 43 1003 236 11
Fragments arranged by size:
1003 236 43 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 839 999 1098 1293
Size: 774 65 160 99 195
Fragments arranged by size:
774 195 160 99 65
TatI w'GTAC_w
Cuts at: 0 242 1132 1206 1293
Size: 242 890 74 87
Fragments arranged by size:
890 242 87 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 291 323 1293
Size: 291 32 970
Fragments arranged by size:
970 291 32
XmnI GAAnn'nnTTC
Cuts at: 0 801 1293
Size: 801 492
Enzymes that do cut:
AcuI AgeI AleI AlwNI ApoI BamHI BanI BanII
BbvCI BciVI BclI BmrI BplI BpmI Bpu10I BsaWI
BseRI BsmBI Bsp1286I BsrDI BsrFI BsrGI BssSI BstXI
BstYI Bsu36I BtsI CspCI DraIII EarI Eco57MI Hin4I
HindIII MfeI MmeI MslI PflMI PstI ScaI SfcI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BspMI BsrBI BssHII BstAPI BstBI
BstEII BstZ17I BtgI BtgZI ClaI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI