Restriction map for
pFN21AE3751
(Linear) (Six-base) MAPSORT of: KIEE3751.seq Check: 6481 from: 1 to: 4407
KIEE3751.orf
With 182 enzymes: SgfI *
March 22, 2012 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 486 4407
Size: 486 3921
AatII G_ACGT'C
Cuts at: 0 3568 4407
Size: 3568 839
AccI GT'mk_AC
Cuts at: 0 275 1127 4407
Size: 275 852 3280
Fragments arranged by size:
3280 852 275
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 260 598 649 1216 2674 2689 2906
Size: 143 117 338 51 567 1458 15 217
Cuts at: 2906 3608 4407
Size: 702 799
Fragments arranged by size:
1458 799 702 567 338 217 143 117
51 15
AfeI AGC'GCT
Cuts at: 0 1673 4407
Size: 1673 2734
AflIII A'CryG_T
Cuts at: 0 3198 4407
Size: 3198 1209
AleI CACnn'nnGTG
Cuts at: 0 385 466 2365 3232 3849 4407
Size: 385 81 1899 867 617 558
Fragments arranged by size:
1899 867 617 558 385 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 974 1907 2248 3916 4407
Size: 421 553 933 341 1668 491
Fragments arranged by size:
1668 933 553 491 421 341
AlwNI CAG_nnn'CTG
Cuts at: 0 121 999 1473 1977 2008 2763 3001 3108
Size: 121 878 474 504 31 755 238 107
Cuts at: 3108 3646 3855 4297 4407
Size: 538 209 442 110
Fragments arranged by size:
878 755 538 504 474 442 238 209
121 110 107 31
ApaI G_GGCC'C
Cuts at: 0 1215 1536 1902 2740 2871 3031 3373 3381
Size: 1215 321 366 838 131 160 342 8
Cuts at: 3381 3753 3804 4407
Size: 372 51 603
Fragments arranged by size:
1215 838 603 372 366 342 321 160
131 51 8
ApoI r'AATT_y
Cuts at: 0 1006 1368 4407
Size: 1006 362 3039
Fragments arranged by size:
3039 1006 362
AseI AT'TA_AT
Cuts at: 0 1399 4407
Size: 1399 3008
AvaI C'yCGr_G
Cuts at: 0 75 109 634 1914 2471 2732 2826 3055
Size: 75 34 525 1280 557 261 94 229
Cuts at: 3055 3356 4407
Size: 301 1051
Fragments arranged by size:
1280 1051 557 525 301 261 229 94
75 34
BamHI G'GATC_C
Cuts at: 0 63 153 1185 4407
Size: 63 90 1032 3222
Fragments arranged by size:
3222 1032 90 63
BanI G'GyrC_C
Cuts at: 0 379 1290 3117 4407
Size: 379 911 1827 1290
Fragments arranged by size:
1827 1290 911 379
BanII G_rGCy'C
Cuts at: 0 82 536 1215 1536 1558 1902 2740 2871
Size: 82 454 679 321 22 344 838 131
Cuts at: 2871 3031 3373 3381 3560 3692 3753 3804 3939
Size: 160 342 8 179 132 61 51 135
Cuts at: 3939 4192 4290 4407
Size: 253 98 117
Fragments arranged by size:
838 679 454 344 342 321 253 179
160 135 132 131 117 98 82 61
51 22 8
BbeI G_GCGC'C
Cuts at: 0 3121 4407
Size: 3121 1286
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 2598 2967 4214 4407
Size: 248 2350 369 1247 193
Fragments arranged by size:
2350 1247 369 248 193
BbvCI CC'TCA_GC
Cuts at: 0 305 1792 2023 2077 3172 4407
Size: 305 1487 231 54 1095 1235
Fragments arranged by size:
1487 1235 1095 305 231 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1863 1897 4407
Size: 1863 34 2510
Fragments arranged by size:
2510 1863 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4320 4407
Size: 4320 87
BglI GCCn_nnn'nGGC
Cuts at: 0 1826 1958 2044 2353 2451 2725 3538 3696
Size: 1826 132 86 309 98 274 813 158
Cuts at: 3696 4141 4407
Size: 445 266
Fragments arranged by size:
1826 813 445 309 274 266 158 132
98 86
BglII A'GATC_T
Cuts at: 0 92 3038 4407
Size: 92 2946 1369
Fragments arranged by size:
2946 1369 92
BlpI GC'TnA_GC
Cuts at: 0 4010 4407
Size: 4010 397
Bme1580I G_kGCm'C
Cuts at: 0 431 1215 1536 1902 2273 2514 2740 2871
Size: 431 784 321 366 371 241 226 131
Cuts at: 2871 2995 3031 3373 3381 3753 3804 4407
Size: 124 36 342 8 372 51 603
Fragments arranged by size:
784 603 431 372 371 366 342 321
241 226 131 124 51 36 8
BmgBI CAC'GTC
Cuts at: 0 4356 4407
Size: 4356 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 795 1218 1305 1434 1461 4051 4407
Size: 567 228 423 87 129 27 2590 356
Fragments arranged by size:
2590 567 423 356 228 129 87 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2216 4407
Size: 2216 2191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 697 712 964 1883 1999 2211 2770
Size: 651 46 15 252 919 116 212 559
Cuts at: 2770 3545 3730 3781 3991 4048 4407
Size: 775 185 51 210 57 359
Fragments arranged by size:
919 775 651 559 359 252 212 210
185 116 57 51 46 15
Bpu10I CC'TnA_GC
Cuts at: 0 305 1792 2023 2032 2077 3172 3554 4407
Size: 305 1487 231 9 45 1095 382 853
Fragments arranged by size:
1487 1095 853 382 305 231 45 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2297 2490 2537 4407
Size: 2297 193 47 1870
Fragments arranged by size:
2297 1870 193 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 1516 2008 2286 2772 3070 3952 4407
Size: 1516 492 278 486 298 882 455
Fragments arranged by size:
1516 882 492 486 455 298 278
BsaAI yAC'GTr
Cuts at: 0 87 4407
Size: 87 4320
BsaBI GATnn'nnATC
Cuts at: 0 1325 4407
Size: 1325 3082
BsaHI Gr'CG_yC
Cuts at: 0 1992 2599 2632 3118 3565 3661 4154 4407
Size: 1992 607 33 486 447 96 493 253
Fragments arranged by size:
1992 607 493 486 447 253 96 33
BsaWI w'CCGG_w
Cuts at: 0 839 1514 1547 1878 2207 3429 3943 4407
Size: 839 675 33 331 329 1222 514 464
Fragments arranged by size:
1222 839 675 514 464 331 329 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1521 1551 3113 3143 3444 3463 3474 3493
Size: 1521 30 1562 30 301 19 11 19
Cuts at: 3493 4407
Size: 914
Fragments arranged by size:
1562 1521 914 301 30 30 19 19
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 544 847 913 916 919 979 1066
Size: 63 481 303 66 3 3 60 87
Cuts at: 1066 1069 1642 1766 1882 2760 3452 4407
Size: 3 573 124 116 878 692 955
Fragments arranged by size:
955 878 692 573 481 303 124 116
87 66 63 60 3 3 3
BseYI C'CCAG_C
Cuts at: 0 367 759 868 969 1770 2384 2756 2820
Size: 367 392 109 101 801 614 372 64
Cuts at: 2820 2914 3142 3381 3408 3456 3639 3779 3855
Size: 94 228 239 27 48 183 140 76
Cuts at: 3855 3915 4407
Size: 60 492
Fragments arranged by size:
801 614 492 392 372 367 239 228
183 140 109 101 94 76 64 60
48 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 914 1102 1903 2043 2167 2188 2259
Size: 848 66 188 801 140 124 21 71
Cuts at: 2259 2326 3945 4242 4407
Size: 67 1619 297 165
Fragments arranged by size:
1619 848 801 297 188 165 140 124
71 67 66 21
BsiEI CG_ry'CG
Cuts at: 0 2817 4018 4022 4407
Size: 2817 1201 4 385
Fragments arranged by size:
2817 1201 385 4
BsiHKAI G_wGCw'C
Cuts at: 0 536 866 1558 1735 2033 2472 2521 2750
Size: 536 330 692 177 298 439 49 229
Cuts at: 2750 3239 3939 3977 4029 4361 4407
Size: 489 700 38 52 332 46
Fragments arranged by size:
700 692 536 489 439 332 330 298
229 177 52 49 46 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1864 4407
Size: 1864 2543
Bsp1286I G_dGCh'C
Cuts at: 0 82 431 536 866 1215 1536 1558 1735
Size: 82 349 105 330 349 321 22 177
Cuts at: 1735 1902 2033 2273 2472 2514 2521 2740 2750
Size: 167 131 240 199 42 7 219 10
Cuts at: 2750 2871 2995 3031 3239 3373 3381 3560 3692
Size: 121 124 36 208 134 8 179 132
Cuts at: 3692 3753 3804 3939 3977 4029 4192 4290 4361
Size: 61 51 135 38 52 163 98 71
Cuts at: 4361 4407
Size: 46
Fragments arranged by size:
349 349 330 321 240 219 208 199
179 177 167 163 135 134 132 131
124 121 105 98 82 71 61 52
51 46 42 38 36 22 10 8
7
BspEI T'CCGG_A
Cuts at: 0 839 1514 4407
Size: 839 675 2893
Fragments arranged by size:
2893 839 675
BspHI T'CATG_A
Cuts at: 0 353 434 1490 4407
Size: 353 81 1056 2917
Fragments arranged by size:
2917 1056 353 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 262 323 486 1875 4407
Size: 262 61 163 1389 2532
Fragments arranged by size:
2532 1389 262 163 61
BsrBI CCG'CTC
Cuts at: 0 909 2853 4014 4407
Size: 909 1944 1161 393
Fragments arranged by size:
1944 1161 909 393
BsrDI GCAATG_nn'
Cuts at: 0 210 4407
Size: 210 4197
BsrFI r'CCGG_y
Cuts at: 0 2407 2547 2650 4017 4407
Size: 2407 140 103 1367 390
Fragments arranged by size:
2407 1367 390 140 103
BssSI C'ACGA_G
Cuts at: 0 494 4407
Size: 494 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 1080 1089 2958 3108 4407
Size: 609 471 9 1869 150 1299
Fragments arranged by size:
1869 1299 609 471 150 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 1182 1459 2610 3990 4407
Size: 675 507 277 1151 1380 417
Fragments arranged by size:
1380 1151 675 507 417 277
BstYI r'GATC_y
Cuts at: 0 63 92 153 360 722 836 1185 3038
Size: 63 29 61 207 362 114 349 1853
Cuts at: 3038 4407
Size: 1369
Fragments arranged by size:
1853 1369 362 349 207 114 63 61
29
Bsu36I CC'TnA_GG
Cuts at: 0 2326 3113 3675 4407
Size: 2326 787 562 732
Fragments arranged by size:
2326 787 732 562
BtgI C'CryG_G
Cuts at: 0 1744 1853 1902 4407
Size: 1744 109 49 2505
Fragments arranged by size:
2505 1744 109 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1319 4407
Size: 1319 3088
BtsI GCAGTG_nn'
Cuts at: 0 133 831 1587 1596 2155 3154 3513 3856
Size: 133 698 756 9 559 999 359 343
Cuts at: 3856 4027 4407
Size: 171 380
Fragments arranged by size:
999 756 698 559 380 359 343 171
133 9
ClaI AT'CG_AT
Cuts at: 0 2399 4407
Size: 2399 2008
DraI TTT'AAA
Cuts at: 0 1275 1373 4407
Size: 1275 98 3034
Fragments arranged by size:
3034 1275 98
DrdI GACnn_nn'nnGTC
Cuts at: 0 3666 4407
Size: 3666 741
EaeI y'GGCC_r
Cuts at: 0 936 2354 2814 3033 3326 3539 3839 4015
Size: 936 1418 460 219 293 213 300 176
Cuts at: 4015 4019 4133 4407
Size: 4 114 274
Fragments arranged by size:
1418 936 460 300 293 274 219 213
176 114 4
EagI C'GGCC_G
Cuts at: 0 2814 4015 4019 4407
Size: 2814 1201 4 388
Fragments arranged by size:
2814 1201 388 4
EarI CTCTTCn'nnn_
Cuts at: 0 743 1280 1373 2539 3890 4407
Size: 743 537 93 1166 1351 517
Fragments arranged by size:
1351 1166 743 537 517 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1542 4407
Size: 1542 2865
EcoICRI GAG'CTC
Cuts at: 0 534 1556 3937 4407
Size: 534 1022 2381 470
Fragments arranged by size:
2381 1022 534 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 260 598 649 651 697 712 964
Size: 143 117 338 51 2 46 15 252
Cuts at: 964 1216 1883 1999 2211 2674 2689 2770 2906
Size: 252 667 116 212 463 15 81 136
Cuts at: 2906 3545 3608 3730 3781 3991 4048 4407
Size: 639 63 122 51 210 57 359
Fragments arranged by size:
667 639 463 359 338 252 252 212
210 143 136 122 117 116 81 63
57 51 51 46 15 15 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2252 2626 3112 4407
Size: 2252 374 486 1295
Fragments arranged by size:
2252 1295 486 374
EcoO109I rG'GnC_Cy
Cuts at: 0 1212 1532 1763 1777 1887 1898 1974 2439
Size: 1212 320 231 14 110 11 76 465
Cuts at: 2439 2475 2736 2830 2867 2868 3028 3081 3105
Size: 36 261 94 37 1 160 53 24
Cuts at: 3105 3302 3369 3370 3377 3378 3668 3697 3749
Size: 197 67 1 7 1 290 29 52
Cuts at: 3749 3750 3800 3801 3966 4407
Size: 1 50 1 165 441
Fragments arranged by size:
1212 465 441 320 290 261 231 197
165 160 110 94 76 67 53 52
50 37 36 29 24 14 11 7
1 1 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 4021 4407
Size: 4021 386
HaeII r_GCGC'y
Cuts at: 0 989 1675 2190 3121 4407
Size: 989 686 515 931 1286
Fragments arranged by size:
1286 989 931 686 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 55 80 87 1170 1202 1521 1553
Size: 48 7 25 7 1083 32 319 32
Cuts at: 1553 3339 3371 4407
Size: 1786 32 1036
Fragments arranged by size:
1786 1083 1036 319 48 32 32 32
25 7 7
HindIII A'AGCT_T
Cuts at: 0 1838 4407
Size: 1838 2569
KasI G'GCGC_C
Cuts at: 0 3117 4407
Size: 3117 1290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2660 4407
Size: 2660 1747
MscI TGG'CCA
Cuts at: 0 2356 3328 3541 4135 4407
Size: 2356 972 213 594 272
Fragments arranged by size:
2356 972 594 272 213
MslI CAynn'nnrTG
Cuts at: 0 385 421 466 2365 3232 3849 4407
Size: 385 36 45 1899 867 617 558
Fragments arranged by size:
1899 867 617 558 385 45 36
MspA1I CmG'CkG
Cuts at: 0 264 1077 1158 1571 1783 1855 2076 2184
Size: 264 813 81 413 212 72 221 108
Cuts at: 2184 2337 2388 2760 3088 3274 3867 4407
Size: 153 51 372 328 186 593 540
Fragments arranged by size:
813 593 540 413 372 328 264 221
212 186 153 108 81 72 51
NaeI GCC'GGC
Cuts at: 0 2409 2549 4019 4407
Size: 2409 140 1470 388
Fragments arranged by size:
2409 1470 388 140
NarI GG'CG_CC
Cuts at: 0 3118 4407
Size: 3118 1289
NcoI C'CATG_G
Cuts at: 0 1744 4407
Size: 1744 2663
NgoMIV G'CCGG_C
Cuts at: 0 2407 2547 4017 4407
Size: 2407 140 1470 390
Fragments arranged by size:
2407 1470 390 140
NspI r_CATG'y
Cuts at: 0 1068 3202 4274 4375 4407
Size: 1068 2134 1072 101 32
Fragments arranged by size:
2134 1072 1068 101 32
PasI CC'CwG_GG
Cuts at: 0 510 2107 3024 3246 3366 3374 3692 4407
Size: 510 1597 917 222 120 8 318 715
Fragments arranged by size:
1597 917 715 510 318 222 120 8
PciI A'CATG_T
Cuts at: 0 3198 4407
Size: 3198 1209
PflMI CCAn_nnn'nTGG
Cuts at: 0 2763 4297 4407
Size: 2763 1534 110
Fragments arranged by size:
2763 1534 110
PfoI T'CCnGG_A
Cuts at: 0 290 725 1280 1697 1860 3094 3607 4064
Size: 290 435 555 417 163 1234 513 457
Cuts at: 4064 4407
Size: 343
Fragments arranged by size:
1234 555 513 457 435 417 343 290
163
PmlI CAC'GTG
Cuts at: 0 87 4407
Size: 87 4320
PpuMI rG'GwC_Cy
Cuts at: 0 1887 2475 2830 3302 3668 4407
Size: 1887 588 355 472 366 739
Fragments arranged by size:
1887 739 588 472 366 355
PshAI GACnn'nnGTC
Cuts at: 0 2598 3211 4407
Size: 2598 613 1196
Fragments arranged by size:
2598 1196 613
PspOMI G'GGCC_C
Cuts at: 0 1211 1532 1898 2736 2867 3027 3369 3377
Size: 1211 321 366 838 131 160 342 8
Cuts at: 3377 3749 3800 4407
Size: 372 51 607
Fragments arranged by size:
1211 838 607 372 366 342 321 160
131 51 8
PspXI vC'TCGA_Gb
Cuts at: 0 634 4407
Size: 634 3773
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 259 291 4407
Size: 259 32 4116
Fragments arranged by size:
4116 259 32
PstI C_TGCA'G
Cuts at: 0 263 605 917 1004 2342 2498 3445 3495
Size: 263 342 312 87 1338 156 947 50
Cuts at: 3495 4407
Size: 912
Fragments arranged by size:
1338 947 912 342 312 263 156 87
50
PvuII CAG'CTG
Cuts at: 0 264 1077 1158 2076 2337 2388 2760 3274
Size: 264 813 81 918 261 51 372 514
Cuts at: 3274 4407
Size: 1133
Fragments arranged by size:
1133 918 813 514 372 264 261 81
51
SacI G_AGCT'C
Cuts at: 0 536 1558 3939 4407
Size: 536 1022 2381 468
Fragments arranged by size:
2381 1022 536 468
SacII CC_GC'GG
Cuts at: 0 1856 4407
Size: 1856 2551
SanDI GG'GwC_CC
Cuts at: 0 2475 2830 3302 3668 4407
Size: 2475 355 472 366 739
Fragments arranged by size:
2475 739 472 366 355
SbfI CC_TGCA'GG
Cuts at: 0 3445 4407
Size: 3445 962
ScaI AGT'ACT
Cuts at: 0 640 4407
Size: 640 3767
SfcI C'TryA_G
Cuts at: 0 259 601 913 1000 2338 2494 3441 3491
Size: 259 342 312 87 1338 156 947 50
Cuts at: 3491 4407
Size: 916
Fragments arranged by size:
1338 947 916 342 312 259 156 87
50
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2353 3538 4141 4407
Size: 2353 1185 603 266
Fragments arranged by size:
2353 1185 603 266
SfoI GGC'GCC
Cuts at: 0 3119 4407
Size: 3119 1288
SmaI CCC'GGG
Cuts at: 0 2734 2828 4407
Size: 2734 94 1579
Fragments arranged by size:
2734 1579 94
SmlI C'TyrA_G
Cuts at: 0 109 634 2312 2505 2552 4407
Size: 109 525 1678 193 47 1855
Fragments arranged by size:
1855 1678 525 193 109 47
SphI G_CATG'C
Cuts at: 0 1068 4375 4407
Size: 1068 3307 32
Fragments arranged by size:
3307 1068 32
SspI AAT'ATT
Cuts at: 0 804 4407
Size: 804 3603
StuI AGG'CCT
Cuts at: 0 568 754 1930 4407
Size: 568 186 1176 2477
Fragments arranged by size:
2477 1176 568 186
StyI C'CwwG_G
Cuts at: 0 221 383 1744 1802 2248 2927 3601 3652
Size: 221 162 1361 58 446 679 674 51
Cuts at: 3652 4149 4407
Size: 497 258
Fragments arranged by size:
1361 679 674 497 446 258 221 162
58 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1408 1445 2185 2463 3222 4407
Size: 1408 37 740 278 759 1185
Fragments arranged by size:
1408 1185 759 740 278 37
TatI w'GTAC_w
Cuts at: 0 638 1688 4407
Size: 638 1050 2719
Fragments arranged by size:
2719 1050 638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3113 3145 3146 3178 3444 3463 3476 3495
Size: 3113 32 1 32 266 19 13 19
Cuts at: 3495 4407
Size: 912
Fragments arranged by size:
3113 912 266 32 32 19 19 13
1
Tth111I GACn'n_nGTC
Cuts at: 0 457 2263 2676 4407
Size: 457 1806 413 1731
Fragments arranged by size:
1806 1731 457 413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 3699 3990 4407
Size: 318 3381 291 417
Fragments arranged by size:
3381 417 318 291
XhoI C'TCGA_G
Cuts at: 0 109 634 4407
Size: 109 525 3773
Fragments arranged by size:
3773 525 109
XmaI C'CCGG_G
Cuts at: 0 2732 2826 4407
Size: 2732 94 1581
Fragments arranged by size:
2732 1581 94
ZraI GAC'GTC
Cuts at: 0 3566 4407
Size: 3566 841
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SacII SanDI SbfI ScaI SfcI SfiI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AloI ApaLI AscI
AsiSI AvrII BaeI BclI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstBI BstEII BstZ17I CspCI DraIII EcoRI
EcoRV FalI FspI FspAI HincII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PsiI PvuI RsrII SalI SapI SexAI SgfI
SgrAI SnaBI SpeI SrfI SwaI XbaI XmnI