(Linear) (Six-base) MAPSORT of: KIEE3730.seq  Check: 4230  from: 1  to: 552



KIEE3730.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      43     156     552 
   Size:         43     113     396

  Fragments arranged by size: 

                 396     113      43
 
ApoI r'AATT_y

Cuts at:      0     486     552 
   Size:        486      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     298     552 
   Size:        298     254
 
BbvCI CC'TCA_GC

Cuts at:      0     330     552 
   Size:        330     222
 
BmgBI CAC'GTC

Cuts at:      0     267     552 
   Size:        267     285
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      31     552 
   Size:         31     521
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     108     552 
   Size:        108     444
 
Bpu10I CC'TnA_GC

Cuts at:      0     330     552 
   Size:        330     222
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     552 
   Size:        146     406
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79     552 
   Size:         79     473
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     552 
   Size:         44      30     478

  Fragments arranged by size: 

                 478      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     552 
   Size:        115     437
 
BsrDI GCAATG_nn'

Cuts at:      0     245     552 
   Size:        245     307
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      21     280     552 
   Size:         21     259     272

  Fragments arranged by size: 

                 272     259      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     372     552 
   Size:        372     180
 
Bsu36I CC'TnA_GG

Cuts at:      0      44     552 
   Size:         44     508
 
BtgI C'CryG_G

Cuts at:      0       6     515     552 
   Size:          6     509      37

  Fragments arranged by size: 

                 509      37       6
 
DraI TTT'AAA

Cuts at:      0     402     552 
   Size:        402     150
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     104     114     530     552 
   Size:         92      12      10     416      22

  Fragments arranged by size: 

                 416      92      22      12      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      47     552 
   Size:         47     505
 
EcoRI G'AATT_C

Cuts at:      0     486     552 
   Size:        486      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176     552 
   Size:        144      32     376

  Fragments arranged by size: 

                 376     144      32
 
MspA1I CmG'CkG

Cuts at:      0       8     334     552 
   Size:          8     326     218

  Fragments arranged by size: 

                 326     218       8
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     257     552 
   Size:        257     295
 
PvuII CAG'CTG

Cuts at:      0     334     552 
   Size:        334     218
 
SacII CC_GC'GG

Cuts at:      0       9     552 
   Size:          9     543
 
SapI GCTCTTCn'nnn_

Cuts at:      0     104     552 
   Size:        104     448
 
SfcI C'TryA_G

Cuts at:      0     226     552 
   Size:        226     326
 
SmlI C'TyrA_G

Cuts at:      0     161     552 
   Size:        161     391
 
StuI AGG'CCT

Cuts at:      0     328     552 
   Size:        328     224
 
Tth111I GACn'n_nGTC

Cuts at:      0      37     156     552 
   Size:         37     119     396

  Fragments arranged by size: 

                 396     119      37
 
XmnI GAAnn'nnTTC

Cuts at:      0     219     552 
   Size:        219     333
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI    BmgBI     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaXI    BseRI    BsrDI   BstAPI    BstXI   Bsu36I
     BtgI     DraI     EarI    EcoNI    EcoRI    Hin4I   MspA1I    PflMI
    PvuII    SacII     SapI     SfcI     SmlI     StuI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmtI     BpmI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI