Restriction map for
pFN21AE3723
(Linear) (Six-base) MAPSORT of: KIEE3723.seq Check: 495 from: 1 to: 2334
KIEE3723.orf
With 182 enzymes: SgfI *
July 3, 2012 18:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1121 1206 1884 2334
Size: 570 551 85 678 450
Fragments arranged by size:
678 570 551 450 85
AfeI AGC'GCT
Cuts at: 0 9 2334
Size: 9 2325
AflII C'TTAA_G
Cuts at: 0 841 2334
Size: 841 1493
AflIII A'CryG_T
Cuts at: 0 815 2334
Size: 815 1519
AhdI GACnn_n'nnGTC
Cuts at: 0 1320 2334
Size: 1320 1014
ApaLI G'TGCA_C
Cuts at: 0 1609 2334
Size: 1609 725
ApoI r'AATT_y
Cuts at: 0 463 595 847 1449 1824 2020 2334
Size: 463 132 252 602 375 196 314
Fragments arranged by size:
602 463 375 314 252 196 132
AvaI C'yCGr_G
Cuts at: 0 455 685 1529 1760 2334
Size: 455 230 844 231 574
Fragments arranged by size:
844 574 455 231 230
AvrII C'CTAG_G
Cuts at: 0 705 2334
Size: 705 1629
BamHI G'GATC_C
Cuts at: 0 736 2334
Size: 736 1598
BanI G'GyrC_C
Cuts at: 0 1353 1659 2278 2334
Size: 1353 306 619 56
Fragments arranged by size:
1353 619 306 56
BanII G_rGCy'C
Cuts at: 0 1096 1740 2080 2090 2334
Size: 1096 644 340 10 244
Fragments arranged by size:
1096 644 340 244 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1279 1396 2074 2334
Size: 1279 117 678 260
Fragments arranged by size:
1279 678 260 117
BglI GCCn_nnn'nGGC
Cuts at: 0 1540 2334
Size: 1540 794
Bme1580I G_kGCm'C
Cuts at: 0 1613 2281 2334
Size: 1613 668 53
Fragments arranged by size:
1613 668 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 233 2334
Size: 233 2101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 2334
Size: 733 1601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 265 1399 2334
Size: 258 7 1134 935
Fragments arranged by size:
1134 935 258 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 748 2334
Size: 151 597 1586
Fragments arranged by size:
1586 597 151
BsaAI yAC'GTr
Cuts at: 0 948 1983 2057 2334
Size: 948 1035 74 277
Fragments arranged by size:
1035 948 277 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 1078 1108 2290 2320 2334
Size: 58 30 990 30 1182 30 14
Fragments arranged by size:
1182 990 58 30 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 261 1324 2334
Size: 154 107 1063 1010
Fragments arranged by size:
1063 1010 154 107
BseYI C'CCAG_C
Cuts at: 0 82 865 1669 1682 1792 1845 2334
Size: 82 783 804 13 110 53 489
Fragments arranged by size:
804 783 489 110 82 53 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 2227 2334
Size: 508 1719 107
Fragments arranged by size:
1719 508 107
BsiHKAI G_wGCw'C
Cuts at: 0 1096 1613 2080 2334
Size: 1096 517 467 254
Fragments arranged by size:
1096 517 467 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1960 2334
Size: 1960 374
Bsp1286I G_dGCh'C
Cuts at: 0 1096 1613 1740 2080 2090 2281 2334
Size: 1096 517 127 340 10 191 53
Fragments arranged by size:
1096 517 340 191 127 53 10
BspHI T'CATG_A
Cuts at: 0 893 2334
Size: 893 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 548 845 2334
Size: 548 297 1489
Fragments arranged by size:
1489 548 297
BsrBI CCG'CTC
Cuts at: 0 1467 2042 2334
Size: 1467 575 292
Fragments arranged by size:
1467 575 292
BsrDI GCAATG_nn'
Cuts at: 0 597 2300 2334
Size: 597 1703 34
Fragments arranged by size:
1703 597 34
BsrFI r'CCGG_y
Cuts at: 0 158 1874 2334
Size: 158 1716 460
Fragments arranged by size:
1716 460 158
BssSI C'ACGA_G
Cuts at: 0 2073 2194 2334
Size: 2073 121 140
Fragments arranged by size:
2073 140 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1827 2334
Size: 1827 507
BstYI r'GATC_y
Cuts at: 0 736 1022 2108 2334
Size: 736 286 1086 226
Fragments arranged by size:
1086 736 286 226
Bsu36I CC'TnA_GG
Cuts at: 0 1307 2334
Size: 1307 1027
BtgI C'CryG_G
Cuts at: 0 821 1001 1538 1565 1640 1663 2081 2236
Size: 821 180 537 27 75 23 418 155
Cuts at: 2236 2285 2334
Size: 49 49
Fragments arranged by size:
821 537 418 180 155 75 49 49
27 23
BtsI GCAGTG_nn'
Cuts at: 0 1734 1998 2215 2334
Size: 1734 264 217 119
Fragments arranged by size:
1734 264 217 119
ClaI AT'CG_AT
Cuts at: 0 903 2334
Size: 903 1431
DraIII CAC_nnn'GTG
Cuts at: 0 1986 2334
Size: 1986 348
EaeI y'GGCC_r
Cuts at: 0 1064 1199 1479 1860 2334
Size: 1064 135 280 381 474
Fragments arranged by size:
1064 474 381 280 135
EarI CTCTTCn'nnn_
Cuts at: 0 664 1013 2334
Size: 664 349 1321
Fragments arranged by size:
1321 664 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 416 457 996 1344 1698 2334
Size: 416 41 539 348 354 636
Fragments arranged by size:
636 539 416 354 348 41
EcoICRI GAG'CTC
Cuts at: 0 1094 2078 2334
Size: 1094 984 256
Fragments arranged by size:
1094 984 256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 265 570 1121 1206 1399 1884 2334
Size: 258 7 305 551 85 193 485 450
Fragments arranged by size:
551 485 450 305 258 193 85 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 2334
Size: 1480 854
EcoO109I rG'GnC_Cy
Cuts at: 0 972 1526 1883 2334
Size: 972 554 357 451
Fragments arranged by size:
972 554 451 357
EcoRI G'AATT_C
Cuts at: 0 595 1449 2334
Size: 595 854 885
Fragments arranged by size:
885 854 595
EcoRV GAT'ATC
Cuts at: 0 2273 2334
Size: 2273 61
HaeII r_GCGC'y
Cuts at: 0 11 1163 1221 2334
Size: 11 1152 58 1113
Fragments arranged by size:
1152 1113 58 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 746 778 1306 1338 2334
Size: 746 32 528 32 996
Fragments arranged by size:
996 746 528 32 32
HindIII A'AGCT_T
Cuts at: 0 2329 2334
Size: 2329 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1827 2334
Size: 119 1708 507
Fragments arranged by size:
1708 507 119
MscI TGG'CCA
Cuts at: 0 1066 1862 2334
Size: 1066 796 472
Fragments arranged by size:
1066 796 472
MslI CAynn'nnrTG
Cuts at: 0 926 2284 2334
Size: 926 1358 50
Fragments arranged by size:
1358 926 50
MspA1I CmG'CkG
Cuts at: 0 734 1141 1215 1567 1665 2135 2238 2334
Size: 734 407 74 352 98 470 103 96
Fragments arranged by size:
734 470 407 352 103 98 96 74
NaeI GCC'GGC
Cuts at: 0 1876 2334
Size: 1876 458
NcoI C'CATG_G
Cuts at: 0 1001 2081 2285 2334
Size: 1001 1080 204 49
Fragments arranged by size:
1080 1001 204 49
NgoMIV G'CCGG_C
Cuts at: 0 1874 2334
Size: 1874 460
NspI r_CATG'y
Cuts at: 0 819 2166 2178 2334
Size: 819 1347 12 156
Fragments arranged by size:
1347 819 156 12
PciI A'CATG_T
Cuts at: 0 815 2334
Size: 815 1519
PfoI T'CCnGG_A
Cuts at: 0 1025 2334
Size: 1025 1309
PmlI CAC'GTG
Cuts at: 0 1983 2057 2334
Size: 1983 74 277
Fragments arranged by size:
1983 277 74
PpuMI rG'GwC_Cy
Cuts at: 0 972 1526 2334
Size: 972 554 808
Fragments arranged by size:
972 808 554
PspXI vC'TCGA_Gb
Cuts at: 0 455 2334
Size: 455 1879
PvuII CAG'CTG
Cuts at: 0 1141 2135 2334
Size: 1141 994 199
Fragments arranged by size:
1141 994 199
SacI G_AGCT'C
Cuts at: 0 1096 2080 2334
Size: 1096 984 254
Fragments arranged by size:
1096 984 254
SacII CC_GC'GG
Cuts at: 0 1568 1666 2239 2334
Size: 1568 98 573 95
Fragments arranged by size:
1568 573 98 95
SanDI GG'GwC_CC
Cuts at: 0 1526 2334
Size: 1526 808
ScaI AGT'ACT
Cuts at: 0 493 2334
Size: 493 1841
SmaI CCC'GGG
Cuts at: 0 1531 1762 2334
Size: 1531 231 572
Fragments arranged by size:
1531 572 231
SmlI C'TyrA_G
Cuts at: 0 166 455 763 841 2334
Size: 166 289 308 78 1493
Fragments arranged by size:
1493 308 289 166 78
SphI G_CATG'C
Cuts at: 0 2178 2334
Size: 2178 156
StuI AGG'CCT
Cuts at: 0 1486 1624 2334
Size: 1486 138 710
Fragments arranged by size:
1486 710 138
StyI C'CwwG_G
Cuts at: 0 59 194 705 1001 1349 2081 2285 2334
Size: 59 135 511 296 348 732 204 49
Fragments arranged by size:
732 511 348 296 204 135 59 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1760 1973 2334
Size: 1760 213 361
Fragments arranged by size:
1760 361 213
TatI w'GTAC_w
Cuts at: 0 491 2159 2334
Size: 491 1668 175
Fragments arranged by size:
1668 491 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 674 706 1576 1608 2334
Size: 58 32 584 32 870 32 726
Fragments arranged by size:
870 726 584 58 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 2079 2334
Size: 201 1878 255
Fragments arranged by size:
1878 255 201
XhoI C'TCGA_G
Cuts at: 0 455 2334
Size: 455 1879
XmaI C'CCGG_G
Cuts at: 0 1529 1760 2334
Size: 1529 231 574
Fragments arranged by size:
1529 574 231
XmnI GAAnn'nnTTC
Cuts at: 0 289 2021 2334
Size: 289 1732 313
Fragments arranged by size:
1732 313 289
Enzymes that do cut:
AcuI AfeI AflII AflIII AhdI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbsI BglI Bme1580I BmrI
BplI BpmI BpuEI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PciI PfoI PmlI PpuMI
PspXI PvuII SacI SacII SanDI ScaI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI Bpu10I BsaI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BspEI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgZI CspCI DraI DrdI EagI FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
RsrII SalI SapI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI ZraI