Restriction map for
pFN21AE3720
(Linear) (Six-base) MAPSORT of: KIEE3720.seq Check: 1148 from: 1 to: 7473
KIEE3720.orf
With 182 enzymes: SgfI *
November 30, 2011 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4432 6961 7473
Size: 4432 2529 512
Fragments arranged by size:
4432 2529 512
AatII G_ACGT'C
Cuts at: 0 3806 6161 7473
Size: 3806 2355 1312
Fragments arranged by size:
3806 2355 1312
Acc65I G'GTAC_C
Cuts at: 0 3091 7473
Size: 3091 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 2378 2546 2999 3586 3739 4022 5889
Size: 775 1603 168 453 587 153 283 1867
Cuts at: 5889 6333 6426 6591 7312 7473
Size: 444 93 165 721 161
Fragments arranged by size:
1867 1603 775 721 587 453 444 283
168 165 161 153 93
AfeI AGC'GCT
Cuts at: 0 61 1447 3541 3753 3973 7473
Size: 61 1386 2094 212 220 3500
Fragments arranged by size:
3500 2094 1386 220 212 61
AflII C'TTAA_G
Cuts at: 0 4593 7473
Size: 4593 2880
AflIII A'CryG_T
Cuts at: 0 2406 2435 3620 7043 7473
Size: 2406 29 1185 3423 430
Fragments arranged by size:
3423 2406 1185 430 29
AhdI GACnn_n'nnGTC
Cuts at: 0 4143 7473
Size: 4143 3330
AleI CACnn'nnGTG
Cuts at: 0 340 1587 2527 3745 7473
Size: 340 1247 940 1218 3728
Fragments arranged by size:
3728 1247 1218 940 340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1856 2502 7473
Size: 1856 646 4971
Fragments arranged by size:
4971 1856 646
AlwNI CAG_nnn'CTG
Cuts at: 0 84 2758 4761 6168 6192 7473
Size: 84 2674 2003 1407 24 1281
Fragments arranged by size:
2674 2003 1407 1281 84 24
ApaI G_GGCC'C
Cuts at: 0 38 3149 6107 7473
Size: 38 3111 2958 1366
Fragments arranged by size:
3111 2958 1366 38
ApaLI G'TGCA_C
Cuts at: 0 448 1778 5514 7473
Size: 448 1330 3736 1959
Fragments arranged by size:
3736 1959 1330 448
ApoI r'AATT_y
Cuts at: 0 328 424 1648 3154 5226 5983 7473
Size: 328 96 1224 1506 2072 757 1490
Fragments arranged by size:
2072 1506 1490 1224 757 328 96
AseI AT'TA_AT
Cuts at: 0 1547 7473
Size: 1547 5926
AvaI C'yCGr_G
Cuts at: 0 102 136 743 1594 6362 6769 6865 7266
Size: 102 34 607 851 4768 407 96 401
Cuts at: 7266 7473
Size: 207
Fragments arranged by size:
4768 851 607 407 401 207 102 96
34
BamHI G'GATC_C
Cuts at: 0 2663 2811 3188 6764 7473
Size: 2663 148 377 3576 709
Fragments arranged by size:
3576 2663 709 377 148
BanI G'GyrC_C
Cuts at: 0 513 580 883 1426 1942 3091 4006 5578
Size: 513 67 303 543 516 1149 915 1572
Cuts at: 5578 5630 7473
Size: 52 1843
Fragments arranged by size:
1843 1572 1149 915 543 516 513 303
67 52
BanII G_rGCy'C
Cuts at: 0 25 38 2011 2291 2518 2987 3149 3586
Size: 25 13 1973 280 227 469 162 437
Cuts at: 3586 4591 6107 7294 7420 7473
Size: 1005 1516 1187 126 53
Fragments arranged by size:
1973 1516 1187 1005 469 437 280 227
162 126 53 25 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 1418 2065 5703 5895 7414 7473
Size: 271 1147 647 3638 192 1519 59
Fragments arranged by size:
3638 1519 1147 647 271 192 59
BbvCI CC'TCA_GC
Cuts at: 0 6822 7195 7473
Size: 6822 373 278
Fragments arranged by size:
6822 373 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4116 4150 4406 4440 6592 6626 7473
Size: 4116 34 256 34 2152 34 847
Fragments arranged by size:
4116 2152 847 256 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2778 7473
Size: 2778 4695
BclI T'GATC_A
Cuts at: 0 566 4826 7473
Size: 566 4260 2647
Fragments arranged by size:
4260 2647 566
BfrBI ATG'CAT
Cuts at: 0 2479 7473
Size: 2479 4994
BglI GCCn_nnn'nGGC
Cuts at: 0 3006 4240 4682 7473
Size: 3006 1234 442 2791
Fragments arranged by size:
3006 2791 1234 442
BglII A'GATC_T
Cuts at: 0 316 7473
Size: 316 7157
BlpI GC'TnA_GC
Cuts at: 0 5250 6928 7473
Size: 5250 1678 545
Fragments arranged by size:
5250 1678 545
Bme1580I G_kGCm'C
Cuts at: 0 38 452 886 1782 3149 4261 5114 5518
Size: 38 414 434 896 1367 1112 853 404
Cuts at: 5518 6107 7473
Size: 589 1366
Fragments arranged by size:
1367 1366 1112 896 853 589 434 414
404 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 2730 4179 4193 7294 7473
Size: 2730 1449 14 3101 179
Fragments arranged by size:
3101 2730 1449 179 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2191 2256 7473
Size: 2191 65 5217
Fragments arranged by size:
5217 2191 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2325 2344 2664 3016 3224 3651 4247 4431
Size: 2325 19 320 352 208 427 596 184
Cuts at: 4431 5666 6531 6587 7473
Size: 1235 865 56 886
Fragments arranged by size:
2325 1235 886 865 596 427 352 320
208 184 56 19
Bpu10I CC'TnA_GC
Cuts at: 0 1263 3295 4810 5324 6822 7195 7473
Size: 1263 2032 1515 514 1498 373 278
Fragments arranged by size:
2032 1515 1498 1263 514 373 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1154 2971 7473
Size: 1154 1817 4502
Fragments arranged by size:
4502 1817 1154
BsaI GGTCTCn'nnnn_
Cuts at: 0 650 1217 3840 3892 5798 7473
Size: 650 567 2623 52 1906 1675
Fragments arranged by size:
2623 1906 1675 650 567 52
BsaAI yAC'GTr
Cuts at: 0 792 2414 2607 3621 4749 5661 6224 7473
Size: 792 1622 193 1014 1128 912 563 1249
Fragments arranged by size:
1622 1249 1128 1014 912 792 563 193
BsaBI GATnn'nnATC
Cuts at: 0 557 2478 7473
Size: 557 1921 4995
Fragments arranged by size:
4995 1921 557
BsaHI Gr'CG_yC
Cuts at: 0 3803 5699 6158 7473
Size: 3803 1896 459 1315
Fragments arranged by size:
3803 1896 1315 459
BsaWI w'CCGG_w
Cuts at: 0 606 2255 5843 7473
Size: 606 1649 3588 1630
Fragments arranged by size:
3588 1649 1630 606
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2323 2353 5117 5147 7473
Size: 2323 30 2764 30 2326
Fragments arranged by size:
2764 2326 2323 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1408 2299 3828 5422 5573 5758 6015 7005
Size: 1408 891 1529 1594 151 185 257 990
Cuts at: 7005 7121 7473
Size: 116 352
Fragments arranged by size:
1594 1529 1408 990 891 352 257 185
151 116
BseYI C'CCAG_C
Cuts at: 0 4010 4284 6192 6353 7279 7473
Size: 4010 274 1908 161 926 194
Fragments arranged by size:
4010 1908 926 274 194 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1344 1634 1863 2607 3484 4860 6321
Size: 165 1179 290 229 744 877 1376 1461
Cuts at: 6321 6415 7382 7473
Size: 94 967 91
Fragments arranged by size:
1461 1376 1179 967 877 744 290 229
165 94 91
BsiEI CG_ry'CG
Cuts at: 0 18 4949 5175 5648 7473
Size: 18 4931 226 473 1825
Fragments arranged by size:
4931 1825 473 226 18
BsiHKAI G_wGCw'C
Cuts at: 0 452 1782 2987 3573 4205 4591 5470 5518
Size: 452 1330 1205 586 632 386 879 48
Cuts at: 5518 6565 6929 7294 7420 7473
Size: 1047 364 365 126 53
Fragments arranged by size:
1330 1205 1047 879 632 586 452 386
365 364 126 53 48
BsmI GAATG_Cn'
Cuts at: 0 1261 2302 6310 7473
Size: 1261 1041 4008 1163
Fragments arranged by size:
4008 1261 1163 1041
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2192 2877 7473
Size: 2192 685 4596
Fragments arranged by size:
4596 2192 685
Bsp1286I G_dGCh'C
Cuts at: 0 25 38 452 886 1782 2011 2291 2518
Size: 25 13 414 434 896 229 280 227
Cuts at: 2518 2987 3149 3573 3586 4205 4261 4591 5114
Size: 469 162 424 13 619 56 330 523
Cuts at: 5114 5470 5518 6107 6565 6929 7294 7420 7473
Size: 356 48 589 458 364 365 126 53
Fragments arranged by size:
896 619 589 523 469 458 434 424
414 365 364 356 330 280 229 227
162 126 56 53 48 25 13 13
BspEI T'CCGG_A
Cuts at: 0 606 7473
Size: 606 6867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1303 2955 3874 4432 6020 6315 6961 7473
Size: 1303 1652 919 558 1588 295 646 512
Fragments arranged by size:
1652 1588 1303 919 646 558 512 295
BsrBI CCG'CTC
Cuts at: 0 873 992 1040 7473
Size: 873 119 48 6433
Fragments arranged by size:
6433 873 119 48
BsrDI GCAATG_nn'
Cuts at: 0 386 3132 7326 7473
Size: 386 2746 4194 147
Fragments arranged by size:
4194 2746 386 147
BsrFI r'CCGG_y
Cuts at: 0 13 2563 7473
Size: 13 2550 4910
Fragments arranged by size:
4910 2550 13
BsrGI T'GTAC_A
Cuts at: 0 4772 6286 7473
Size: 4772 1514 1187
Fragments arranged by size:
4772 1514 1187
BssSI C'ACGA_G
Cuts at: 0 1583 2658 5463 5809 6781 7473
Size: 1583 1075 2805 346 972 692
Fragments arranged by size:
2805 1583 1075 972 692 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 1612 5109 7473
Size: 84 1528 3497 2364
Fragments arranged by size:
3497 2364 1528 84
BstBI TT'CG_AA
Cuts at: 0 5360 7473
Size: 5360 2113
BstEII G'GTnAC_C
Cuts at: 0 4551 7473
Size: 4551 2922
BstXI CCAn_nnnn'nTGG
Cuts at: 0 7428 7473
Size: 7428 45
BstYI r'GATC_y
Cuts at: 0 316 2663 2811 3188 3493 6521 6764 7118
Size: 316 2347 148 377 305 3028 243 354
Cuts at: 7118 7473
Size: 355
Fragments arranged by size:
3028 2347 377 355 354 316 305 243
148
Bsu36I CC'TnA_GG
Cuts at: 0 4847 6386 7473
Size: 4847 1539 1087
Fragments arranged by size:
4847 1539 1087
BtgI C'CryG_G
Cuts at: 0 2387 3482 4002 4469 7135 7473
Size: 2387 1095 520 467 2666 338
Fragments arranged by size:
2666 2387 1095 520 467 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2648 4987 5268 7473
Size: 2648 2339 281 2205
Fragments arranged by size:
2648 2339 2205 281
BtsI GCAGTG_nn'
Cuts at: 0 470 692 1613 1622 3419 7473
Size: 470 222 921 9 1797 4054
Fragments arranged by size:
4054 1797 921 470 222 9
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 186 221 558 593 6278 6313 7473
Size: 186 35 337 35 5685 35 1160
Fragments arranged by size:
5685 1160 337 186 35 35 35
DraI TTT'AAA
Cuts at: 0 498 7473
Size: 498 6975
DraIII CAC_nnn'GTG
Cuts at: 0 1563 3879 5109 7302 7473
Size: 1563 2316 1230 2193 171
Fragments arranged by size:
2316 2193 1563 1230 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 778 3253 3334 4549 5560 6163 7473
Size: 778 2475 81 1215 1011 603 1310
Fragments arranged by size:
2475 1310 1215 1011 778 603 81
EaeI y'GGCC_r
Cuts at: 0 15 735 4015 5172 7164 7473
Size: 15 720 3280 1157 1992 309
Fragments arranged by size:
3280 1992 1157 720 309 15
EagI C'GGCC_G
Cuts at: 0 15 5172 7473
Size: 15 5157 2301
Fragments arranged by size:
5157 2301 15
EarI CTCTTCn'nnn_
Cuts at: 0 530 794 2225 2975 4519 4912 5728 6687
Size: 530 264 1431 750 1544 393 816 959
Cuts at: 6687 6811 7073 7109 7473
Size: 124 262 36 364
Fragments arranged by size:
1544 1431 959 816 750 530 393 364
264 262 124 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1459 7473
Size: 1459 6014
EcoICRI GAG'CTC
Cuts at: 0 2985 4589 7292 7418 7473
Size: 2985 1604 2703 126 55
Fragments arranged by size:
2985 2703 1604 126 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 2325 2344 2378 2546 2664 2999 3016
Size: 775 1550 19 34 168 118 335 17
Cuts at: 3016 3224 3586 3651 3739 4022 4247 4431 5666
Size: 208 362 65 88 283 225 184 1235
Cuts at: 5666 5889 6333 6426 6531 6587 6591 7312 7473
Size: 223 444 93 105 56 4 721 161
Fragments arranged by size:
1550 1235 775 721 444 362 335 283
225 223 208 184 168 161 118 105
93 88 65 56 34 19 17 4
EcoO109I rG'GnC_Cy
Cuts at: 0 34 3073 3145 3146 4194 4691 4758 4784
Size: 34 3039 72 1 1048 497 67 26
Cuts at: 4784 6038 6103 6104 6165 7458 7473
Size: 1254 65 1 61 1293 15
Fragments arranged by size:
3039 1293 1254 1048 497 72 67 65
61 34 26 15 1 1
EcoRI G'AATT_C
Cuts at: 0 328 7473
Size: 328 7145
EcoRV GAT'ATC
Cuts at: 0 3171 3578 5169 6327 7473
Size: 3171 407 1591 1158 1146
Fragments arranged by size:
3171 1591 1158 1146 407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 834 995 1989 2383 7473
Size: 834 161 994 394 5090
Fragments arranged by size:
5090 994 834 394 161
FspI TGC'GCA
Cuts at: 0 766 7473
Size: 766 6707
HaeII r_GCGC'y
Cuts at: 0 63 1449 3543 3755 3975 4469 6949 7473
Size: 63 1386 2094 212 220 494 2480 524
Fragments arranged by size:
2480 2094 1386 524 494 220 212 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1998 2030 2500 2532 2652 2684 2855 2887
Size: 1998 32 470 32 120 32 171 32
Cuts at: 2887 3124 3156 3160 3192 4129 4161 4573 4605
Size: 237 32 4 32 937 32 412 32
Cuts at: 4605 4887 4919 5032 5064 7473
Size: 282 32 113 32 2409
Fragments arranged by size:
2409 1998 937 470 412 282 237 171
120 113 32 32 32 32 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 1356 3327 3516 4406 4983 7473
Size: 1356 1971 189 890 577 2490
Fragments arranged by size:
2490 1971 1356 890 577 189
HindIII A'AGCT_T
Cuts at: 0 571 2089 3790 7473
Size: 571 1518 1701 3683
Fragments arranged by size:
3683 1701 1518 571
KpnI G_GTAC'C
Cuts at: 0 3095 7473
Size: 3095 4378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 935 2139 2994 5954 6549 6592 6600
Size: 848 87 1204 855 2960 595 43 8
Cuts at: 6600 7064 7473
Size: 464 409
Fragments arranged by size:
2960 1204 855 848 595 464 409 87
43 8
MscI TGG'CCA
Cuts at: 0 737 7473
Size: 737 6736
MslI CAynn'nnrTG
Cuts at: 0 340 1587 1752 2434 2527 3745 5636 5937
Size: 340 1247 165 682 93 1218 1891 301
Cuts at: 5937 7211 7473
Size: 1274 262
Fragments arranged by size:
1891 1274 1247 1218 682 340 301 262
165 93
MspA1I CmG'CkG
Cuts at: 0 81 672 1275 1840 2585 3036 3097 3484
Size: 81 591 603 565 745 451 61 387
Cuts at: 3484 4004 4014 4618 4767 4929 5131 6335 6353
Size: 520 10 604 149 162 202 1204 18
Cuts at: 6353 7125 7473
Size: 772 348
Fragments arranged by size:
1204 772 745 604 603 591 565 520
451 387 348 202 162 149 81 61
18 10
NaeI GCC'GGC
Cuts at: 0 15 7473
Size: 15 7458
NcoI C'CATG_G
Cuts at: 0 2387 7473
Size: 2387 5086
NdeI CA'TA_TG
Cuts at: 0 6577 7473
Size: 6577 896
NgoMIV G'CCGG_C
Cuts at: 0 13 7473
Size: 13 7460
NruI TCG'CGA
Cuts at: 0 5486 7473
Size: 5486 1987
NsiI A_TGCA'T
Cuts at: 0 2481 7473
Size: 2481 4992
NspI r_CATG'y
Cuts at: 0 2410 4901 7473
Size: 2410 2491 2572
Fragments arranged by size:
2572 2491 2410
PasI CC'CwG_GG
Cuts at: 0 411 3726 6108 7473
Size: 411 3315 2382 1365
Fragments arranged by size:
3315 2382 1365 411
PciI A'CATG_T
Cuts at: 0 2406 7473
Size: 2406 5067
PflMI CCAn_nnn'nTGG
Cuts at: 0 3700 6168 7473
Size: 3700 2468 1305
Fragments arranged by size:
3700 2468 1305
PfoI T'CCnGG_A
Cuts at: 0 323 3393 7473
Size: 323 3070 4080
Fragments arranged by size:
4080 3070 323
PmlI CAC'GTG
Cuts at: 0 2414 7473
Size: 2414 5059
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 1061 7473
Size: 1029 32 6412
Fragments arranged by size:
6412 1029 32
PpuMI rG'GwC_Cy
Cuts at: 0 4194 4758 4784 6038 6165 7458 7473
Size: 4194 564 26 1254 127 1293 15
Fragments arranged by size:
4194 1293 1254 564 127 26 15
PshAI GACnn'nnGTC
Cuts at: 0 4379 5374 7473
Size: 4379 995 2099
Fragments arranged by size:
4379 2099 995
PsiI TTA'TAA
Cuts at: 0 191 7473
Size: 191 7282
PspOMI G'GGCC_C
Cuts at: 0 34 3145 6103 7473
Size: 34 3111 2958 1370
Fragments arranged by size:
3111 2958 1370 34
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 966 998 7020 7052 7473
Size: 966 32 6022 32 421
Fragments arranged by size:
6022 966 421 32 32
PstI C_TGCA'G
Cuts at: 0 80 477 1140 1571 4429 4766 5827 7473
Size: 80 397 663 431 2858 337 1061 1646
Fragments arranged by size:
2858 1646 1061 663 431 397 337 80
PvuII CAG'CTG
Cuts at: 0 81 672 1840 3036 4618 4767 6335 6353
Size: 81 591 1168 1196 1582 149 1568 18
Cuts at: 6353 7125 7473
Size: 772 348
Fragments arranged by size:
1582 1568 1196 1168 772 591 348 149
81 18
SacI G_AGCT'C
Cuts at: 0 2987 4591 7294 7420 7473
Size: 2987 1604 2703 126 53
Fragments arranged by size:
2987 2703 1604 126 53
SacII CC_GC'GG
Cuts at: 0 3485 4005 7473
Size: 3485 520 3468
Fragments arranged by size:
3485 3468 520
SanDI GG'GwC_CC
Cuts at: 0 4194 4784 7473
Size: 4194 590 2689
Fragments arranged by size:
4194 2689 590
SapI GCTCTTCn'nnn_
Cuts at: 0 2975 4519 5728 7473
Size: 2975 1544 1209 1745
Fragments arranged by size:
2975 1745 1544 1209
ScaI AGT'ACT
Cuts at: 0 5241 6643 7473
Size: 5241 1402 830
Fragments arranged by size:
5241 1402 830
SexAI A'CCwGG_T
Cuts at: 0 4754 7473
Size: 4754 2719
SfcI C'TryA_G
Cuts at: 0 76 473 1136 1567 2124 4425 4762 5436
Size: 76 397 663 431 557 2301 337 674
Cuts at: 5436 5499 5823 7026 7473
Size: 63 324 1203 447
Fragments arranged by size:
2301 1203 674 663 557 447 431 397
337 324 76 63
SmaI CCC'GGG
Cuts at: 0 104 745 6364 6867 7473
Size: 104 641 5619 503 606
Fragments arranged by size:
5619 641 606 503 104
SmlI C'TyrA_G
Cuts at: 0 1169 2986 4593 7473
Size: 1169 1817 1607 2880
Fragments arranged by size:
2880 1817 1607 1169
SspI AAT'ATT
Cuts at: 0 1075 1551 5079 7473
Size: 1075 476 3528 2394
Fragments arranged by size:
3528 2394 1075 476
StuI AGG'CCT
Cuts at: 0 4845 7473
Size: 4845 2628
StyI C'CwwG_G
Cuts at: 0 2367 2387 3313 3694 3827 5582 7473
Size: 2367 20 926 381 133 1755 1891
Fragments arranged by size:
2367 1891 1755 926 381 133 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 2323 3445 4024 4276 4963 5909 6184
Size: 277 2046 1122 579 252 687 946 275
Cuts at: 6184 7313 7473
Size: 1129 160
Fragments arranged by size:
2046 1129 1122 946 687 579 277 275
252 160
TatI w'GTAC_w
Cuts at: 0 183 4484 4772 5239 6286 6641 7473
Size: 183 4301 288 467 1047 355 832
Fragments arranged by size:
4301 1047 832 467 355 288 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3441 3473 4500 4532 5117 5149 7473
Size: 3441 32 1027 32 585 32 2324
Fragments arranged by size:
3441 2324 1027 585 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4756 5698 6711 7473
Size: 4756 942 1013 762
Fragments arranged by size:
4756 1013 942 762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 1490 2544 4716 7473
Size: 321 1169 1054 2172 2757
Fragments arranged by size:
2757 2172 1169 1054 321
XmaI C'CCGG_G
Cuts at: 0 102 743 6362 6865 7473
Size: 102 641 5619 503 608
Fragments arranged by size:
5619 641 608 503 102
ZraI GAC'GTC
Cuts at: 0 3804 6159 7473
Size: 3804 2355 1314
Fragments arranged by size:
3804 2355 1314
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuII SacI SacII SanDI SapI
ScaI SexAI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AgeI AloI AscI AsiSI AvrII BaeI
BbeI BmgBI BmtI BsiWI BspHI BssHII BstZ17I ClaI
EcoNI FseI FspAI HpaI KasI MfeI MluI NarI
NheI NotI PacI PmeI PspXI PvuI RsrII SalI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI XbaI XhoI XmnI