Restriction map for
pFN21AE3719
(Linear) (Six-base) MAPSORT of: KIEE3719.seq Check: 7584 from: 1 to: 6294
KIEE3719.orf
With 182 enzymes: SgfI *
February 2, 2012 14:09 ..
AatII G_ACGT'C
Cuts at: 0 22 6294
Size: 22 6272
AccI GT'mk_AC
Cuts at: 0 4048 6294
Size: 4048 2246
Acc65I G'GTAC_C
Cuts at: 0 960 6258 6294
Size: 960 5298 36
Fragments arranged by size:
5298 960 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1463 2170 3122 3322 3648 3907 4213
Size: 296 1167 707 952 200 326 259 306
Cuts at: 4213 4258 4547 4639 4766 5099 5223 6294
Size: 45 289 92 127 333 124 1071
Fragments arranged by size:
1167 1071 952 707 333 326 306 296
289 259 200 127 124 92 45
AflII C'TTAA_G
Cuts at: 0 651 6294
Size: 651 5643
AflIII A'CryG_T
Cuts at: 0 1605 2285 6294
Size: 1605 680 4009
Fragments arranged by size:
4009 1605 680
AhdI GACnn_n'nnGTC
Cuts at: 0 1884 1951 6294
Size: 1884 67 4343
Fragments arranged by size:
4343 1884 67
AleI CACnn'nnGTG
Cuts at: 0 1097 6294
Size: 1097 5197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 899 2864 6294
Size: 899 1965 3430
Fragments arranged by size:
3430 1965 899
AlwNI CAG_nnn'CTG
Cuts at: 0 715 3910 4236 4446 5590 6294
Size: 715 3195 326 210 1144 704
Fragments arranged by size:
3195 1144 715 704 326 210
ApaI G_GGCC'C
Cuts at: 0 4778 6294
Size: 4778 1516
ApoI r'AATT_y
Cuts at: 0 508 595 1040 1327 2108 2197 2425 2626
Size: 508 87 445 287 781 89 228 201
Cuts at: 2626 3280 3482 3495 3733 4887 5735 6164 6294
Size: 654 202 13 238 1154 848 429 130
Fragments arranged by size:
1154 848 781 654 508 445 429 287
238 228 202 201 130 89 87 13
AseI AT'TA_AT
Cuts at: 0 2318 2647 3638 6294
Size: 2318 329 991 2656
Fragments arranged by size:
2656 2318 991 329
AvaI C'yCGr_G
Cuts at: 0 2549 3230 5570 6294
Size: 2549 681 2340 724
Fragments arranged by size:
2549 2340 724 681
AvrII C'CTAG_G
Cuts at: 0 2502 6294
Size: 2502 3792
BamHI G'GATC_C
Cuts at: 0 2739 3018 4834 6294
Size: 2739 279 1816 1460
Fragments arranged by size:
2739 1816 1460 279
BanI G'GyrC_C
Cuts at: 0 960 4040 6258 6294
Size: 960 3080 2218 36
Fragments arranged by size:
3080 2218 960 36
BanII G_rGCy'C
Cuts at: 0 119 1781 4073 4708 4778 5863 6294
Size: 119 1662 2292 635 70 1085 431
Fragments arranged by size:
2292 1662 1085 635 431 119 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 4412 4590 6294
Size: 698 3714 178 1704
Fragments arranged by size:
3714 1704 698 178
BbvCI CC'TCA_GC
Cuts at: 0 230 391 4439 6294
Size: 230 161 4048 1855
Fragments arranged by size:
4048 1855 230 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5620 5654 6294
Size: 5620 34 640
Fragments arranged by size:
5620 640 34
BclI T'GATC_A
Cuts at: 0 105 6294
Size: 105 6189
BfrBI ATG'CAT
Cuts at: 0 3526 4627 6294
Size: 3526 1101 1667
Fragments arranged by size:
3526 1667 1101
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 6294
Size: 1207 5087
BglII A'GATC_T
Cuts at: 0 943 5796 6294
Size: 943 4853 498
Fragments arranged by size:
4853 943 498
BlpI GC'TnA_GC
Cuts at: 0 3416 4067 6294
Size: 3416 651 2227
Fragments arranged by size:
3416 2227 651
Bme1580I G_kGCm'C
Cuts at: 0 1212 1613 2416 4043 4778 4976 6294
Size: 1212 401 803 1627 735 198 1318
Fragments arranged by size:
1627 1318 1212 803 735 401 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 3166 3675 6294
Size: 3166 509 2619
Fragments arranged by size:
3166 2619 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1676 1795 1843 3887 4631 6294
Size: 283 1393 119 48 2044 744 1663
Fragments arranged by size:
2044 1663 1393 744 283 119 48
Bpu10I CC'TnA_GC
Cuts at: 0 230 391 3319 4439 4781 6294
Size: 230 161 2928 1120 342 1513
Fragments arranged by size:
2928 1513 1120 342 230 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 5354 6160 6294
Size: 397 4957 806 134
Fragments arranged by size:
4957 806 397 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 608 1012 2902 3041 3266 4551 5281 6294
Size: 608 404 1890 139 225 1285 730 1013
Fragments arranged by size:
1890 1285 1013 730 608 404 225 139
BsaAI yAC'GTr
Cuts at: 0 5296 6294
Size: 5296 998
BsaBI GATnn'nnATC
Cuts at: 0 5930 6294
Size: 5930 364
BsaHI Gr'CG_yC
Cuts at: 0 19 1339 4132 6294
Size: 19 1320 2793 2162
Fragments arranged by size:
2793 2162 1320 19
BsaWI w'CCGG_w
Cuts at: 0 2925 6204 6294
Size: 2925 3279 90
Fragments arranged by size:
3279 2925 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1329 1359 6294
Size: 1329 30 4935
Fragments arranged by size:
4935 1329 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 839 1804 2540 3087 3254 3259 3488 5402
Size: 839 965 736 547 167 5 229 1914
Cuts at: 5402 6294
Size: 892
Fragments arranged by size:
1914 965 892 839 736 547 229 167
5
BseYI C'CCAG_C
Cuts at: 0 886 4830 4999 6294
Size: 886 3944 169 1295
Fragments arranged by size:
3944 1295 886 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 3895 6294
Size: 1078 2817 2399
Fragments arranged by size:
2817 2399 1078
BsiHKAI G_wGCw'C
Cuts at: 0 1781 3406 3662 4708 6294
Size: 1781 1625 256 1046 1586
Fragments arranged by size:
1781 1625 1586 1046 256
BsmI GAATG_Cn'
Cuts at: 0 705 3455 6294
Size: 705 2750 2839
Fragments arranged by size:
2839 2750 705
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1330 6294
Size: 1330 4964
Bsp1286I G_dGCh'C
Cuts at: 0 119 1212 1613 1781 2416 3406 3662 4043
Size: 119 1093 401 168 635 990 256 381
Cuts at: 4043 4073 4708 4778 4976 5863 6294
Size: 30 635 70 198 887 431
Fragments arranged by size:
1093 990 887 635 635 431 401 381
256 198 168 119 70 30
BspEI T'CCGG_A
Cuts at: 0 6204 6294
Size: 6204 90
BspHI T'CATG_A
Cuts at: 0 357 4092 6294
Size: 357 3735 2202
Fragments arranged by size:
3735 2202 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3240 6294
Size: 3240 3054
BsrDI GCAATG_nn'
Cuts at: 0 534 1241 2066 3696 4469 4976 5009 6087
Size: 534 707 825 1630 773 507 33 1078
Cuts at: 6087 6242 6294
Size: 155 52
Fragments arranged by size:
1630 1078 825 773 707 534 507 155
52 33
BsrGI T'GTAC_A
Cuts at: 0 2509 6212 6294
Size: 2509 3703 82
Fragments arranged by size:
3703 2509 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 569 5308 6294
Size: 569 4739 986
Fragments arranged by size:
4739 986 569
BstBI TT'CG_AA
Cuts at: 0 384 6114 6294
Size: 384 5730 180
Fragments arranged by size:
5730 384 180
BstEII G'GTnAC_C
Cuts at: 0 260 6294
Size: 260 6034
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5727 6269 6294
Size: 5727 542 25
Fragments arranged by size:
5727 542 25
BstYI r'GATC_y
Cuts at: 0 943 2561 2739 3018 3324 4233 4834 5796
Size: 943 1618 178 279 306 909 601 962
Cuts at: 5796 6294
Size: 498
Fragments arranged by size:
1618 962 943 909 601 498 306 279
178
BstZ17I GTA'TAC
Cuts at: 0 4049 6294
Size: 4049 2245
Bsu36I CC'TnA_GG
Cuts at: 0 1783 6294
Size: 1783 4511
BtgI C'CryG_G
Cuts at: 0 1370 1811 3830 4633 5720 6294
Size: 1370 441 2019 803 1087 574
Fragments arranged by size:
2019 1370 1087 803 574 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4633 6294
Size: 4633 1661
BtsI GCAGTG_nn'
Cuts at: 0 1104 1312 5526 6294
Size: 1104 208 4214 768
Fragments arranged by size:
4214 1104 768 208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3599 3634 6294
Size: 3599 35 2660
Fragments arranged by size:
3599 2660 35
DraI TTT'AAA
Cuts at: 0 90 126 1401 6294
Size: 90 36 1275 4893
Fragments arranged by size:
4893 1275 90 36
EaeI y'GGCC_r
Cuts at: 0 2 3455 3543 3861 5729 6294
Size: 2 3453 88 318 1868 565
Fragments arranged by size:
3453 1868 565 318 88 2
EarI CTCTTCn'nnn_
Cuts at: 0 315 3569 3667 3678 5275 5277 6294
Size: 315 3254 98 11 1597 2 1017
Fragments arranged by size:
3254 1597 1017 315 98 11 2
EcoICRI GAG'CTC
Cuts at: 0 1779 4706 6294
Size: 1779 2927 1588
Fragments arranged by size:
2927 1779 1588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 296 1463 1676 1795 1843 2170 3122
Size: 283 13 1167 213 119 48 327 952
Cuts at: 3122 3322 3648 3887 3907 4213 4258 4547 4631
Size: 200 326 239 20 306 45 289 84
Cuts at: 4631 4639 4766 5099 5223 6294
Size: 8 127 333 124 1071
Fragments arranged by size:
1167 1071 952 333 327 326 306 289
283 239 213 200 127 124 119 84
48 45 20 13 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1786 2677 4228 6294
Size: 1786 891 1551 2066
Fragments arranged by size:
2066 1786 1551 891
EcoO109I rG'GnC_Cy
Cuts at: 0 4206 4527 4774 4775 6294
Size: 4206 321 247 1 1519
Fragments arranged by size:
4206 1519 321 247 1
EcoRI G'AATT_C
Cuts at: 0 2197 3280 3482 6294
Size: 2197 1083 202 2812
Fragments arranged by size:
2812 2197 1083 202
EcoRV GAT'ATC
Cuts at: 0 1980 3916 6294
Size: 1980 1936 2378
Fragments arranged by size:
2378 1980 1936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1700 4240 4263 5113 6294
Size: 1700 2540 23 850 1181
Fragments arranged by size:
2540 1700 1181 850 23
HaeII r_GCGC'y
Cuts at: 0 309 3852 6294
Size: 309 3543 2442
Fragments arranged by size:
3543 2442 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 90 122 213 245 1125 1157
Size: 4 32 54 32 91 32 880 32
Cuts at: 1157 1329 1342 1361 1374 1513 1545 1652 1684
Size: 172 13 19 13 139 32 107 32
Cuts at: 1684 1711 1743 1870 1902 1937 1969 2714 2746
Size: 27 32 127 32 35 32 745 32
Cuts at: 2746 3982 4014 5044 5076 5337 5369 6294
Size: 1236 32 1030 32 261 32 925
Fragments arranged by size:
1236 1030 925 880 745 261 172 139
127 107 91 54 35 32 32 32
32 32 32 32 32 32 32 32
32 32 27 19 13 13 4
HincII GTy'rAC
Cuts at: 0 1173 3611 4865 6294
Size: 1173 2438 1254 1429
Fragments arranged by size:
2438 1429 1254 1173
HindIII A'AGCT_T
Cuts at: 0 4505 6136 6294
Size: 4505 1631 158
Fragments arranged by size:
4505 1631 158
KpnI G_GTAC'C
Cuts at: 0 964 6262 6294
Size: 964 5298 32
Fragments arranged by size:
5298 964 32
MfeI C'AATT_G
Cuts at: 0 1223 4729 6294
Size: 1223 3506 1565
Fragments arranged by size:
3506 1565 1223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1968 3875 4560 5125 6075 6294
Size: 199 1769 1907 685 565 950 219
Fragments arranged by size:
1907 1769 950 685 565 219 199
MscI TGG'CCA
Cuts at: 0 4 3545 3863 5731 6294
Size: 4 3541 318 1868 563
Fragments arranged by size:
3541 1868 563 318 4
MslI CAynn'nnrTG
Cuts at: 0 1097 1909 2647 6123 6294
Size: 1097 812 738 3476 171
Fragments arranged by size:
3476 1097 812 738 171
MspA1I CmG'CkG
Cuts at: 0 303 373 2391 3315 6294
Size: 303 70 2018 924 2979
Fragments arranged by size:
2979 2018 924 303 70
NcoI C'CATG_G
Cuts at: 0 1811 3830 6294
Size: 1811 2019 2464
Fragments arranged by size:
2464 2019 1811
NdeI CA'TA_TG
Cuts at: 0 969 1005 6294
Size: 969 36 5289
Fragments arranged by size:
5289 969 36
NsiI A_TGCA'T
Cuts at: 0 3528 4629 6294
Size: 3528 1101 1665
Fragments arranged by size:
3528 1665 1101
NspI r_CATG'y
Cuts at: 0 1609 2289 3816 6294
Size: 1609 680 1527 2478
Fragments arranged by size:
2478 1609 1527 680
PasI CC'CwG_GG
Cuts at: 0 2735 6294
Size: 2735 3559
PciI A'CATG_T
Cuts at: 0 1605 2285 6294
Size: 1605 680 4009
Fragments arranged by size:
4009 1605 680
PflMI CCAn_nnn'nTGG
Cuts at: 0 1848 2735 3441 3910 4128 4579 6294
Size: 1848 887 706 469 218 451 1715
Fragments arranged by size:
1848 1715 887 706 469 451 218
PfoI T'CCnGG_A
Cuts at: 0 4578 4647 4896 6294
Size: 4578 69 249 1398
Fragments arranged by size:
4578 1398 249 69
PpuMI rG'GwC_Cy
Cuts at: 0 4527 6294
Size: 4527 1767
PshAI GACnn'nnGTC
Cuts at: 0 979 6294
Size: 979 5315
PsiI TTA'TAA
Cuts at: 0 275 5762 6294
Size: 275 5487 532
Fragments arranged by size:
5487 532 275
PspOMI G'GGCC_C
Cuts at: 0 4774 6294
Size: 4774 1520
PspXI vC'TCGA_Gb
Cuts at: 0 2549 6294
Size: 2549 3745
PstI C_TGCA'G
Cuts at: 0 565 1319 3017 4451 6294
Size: 565 754 1698 1434 1843
Fragments arranged by size:
1843 1698 1434 754 565
PvuII CAG'CTG
Cuts at: 0 373 2391 3315 6294
Size: 373 2018 924 2979
Fragments arranged by size:
2979 2018 924 373
SacI G_AGCT'C
Cuts at: 0 1781 4708 6294
Size: 1781 2927 1586
Fragments arranged by size:
2927 1781 1586
SapI GCTCTTCn'nnn_
Cuts at: 0 315 3667 3678 6294
Size: 315 3352 11 2616
Fragments arranged by size:
3352 2616 315 11
ScaI AGT'ACT
Cuts at: 0 3534 5112 6294
Size: 3534 1578 1182
Fragments arranged by size:
3534 1578 1182
SexAI A'CCwGG_T
Cuts at: 0 5318 6294
Size: 5318 976
SfcI C'TryA_G
Cuts at: 0 498 561 1217 1315 2142 3013 3055 4427
Size: 498 63 656 98 827 871 42 1372
Cuts at: 4427 4447 6294
Size: 20 1847
Fragments arranged by size:
1847 1372 871 827 656 498 98 63
42 20
SmaI CCC'GGG
Cuts at: 0 5572 6294
Size: 5572 722
SmlI C'TyrA_G
Cuts at: 0 412 651 2549 5369 6139 6294
Size: 412 239 1898 2820 770 155
Fragments arranged by size:
2820 1898 770 412 239 155
SpeI A'CTAG_T
Cuts at: 0 64 6294
Size: 64 6230
SspI AAT'ATT
Cuts at: 0 2570 3767 4190 4641 6294
Size: 2570 1197 423 451 1653
Fragments arranged by size:
2570 1653 1197 451 423
StuI AGG'CCT
Cuts at: 0 3792 6294
Size: 3792 2502
StyI C'CwwG_G
Cuts at: 0 1811 2243 2361 2502 3715 3830 3864 6294
Size: 1811 432 118 141 1213 115 34 2430
Fragments arranged by size:
2430 1811 1213 432 141 118 115 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 279 4443 5362 6294
Size: 279 4164 919 932
Fragments arranged by size:
4164 932 919 279
TatI w'GTAC_w
Cuts at: 0 1321 2509 3532 5110 6212 6294
Size: 1321 1188 1023 1578 1102 82
Fragments arranged by size:
1578 1321 1188 1102 1023 82
Tth111I GACn'n_nGTC
Cuts at: 0 249 6294
Size: 249 6045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1096 6294
Size: 1096 5198
XhoI C'TCGA_G
Cuts at: 0 2549 6294
Size: 2549 3745
XmaI C'CCGG_G
Cuts at: 0 5570 6294
Size: 5570 724
XmnI GAAnn'nnTTC
Cuts at: 0 3194 4243 5096 5507 6294
Size: 3194 1049 853 411 787
Fragments arranged by size:
3194 1049 853 787 411
ZraI GAC'GTC
Cuts at: 0 20 6294
Size: 20 6274
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AseI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI
SapI ScaI SexAI SfcI SmaI SmlI SpeI SspI
StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AloI ApaLI AscI AsiSI
BaeI BbeI BciVI BmgBI BmtI BplI BsiEI BsiWI
BsrBI BsrFI BssHII BssSI ClaI DraIII DrdI EagI
EciI FseI FspI FspAI HpaI KasI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PmeI PmlI
PpiI PsrI PvuI RsrII SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SwaI
TstI XbaI