Restriction map for
pFN21AE3714
(Linear) (Six-base) MAPSORT of: KIEE3714.seq Check: 8688 from: 1 to: 6510
KIEE3714.orf
With 182 enzymes: SgfI *
March 22, 2012 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3088 6510
Size: 3088 3422
AatII G_ACGT'C
Cuts at: 0 4700 6510
Size: 4700 1810
AccI GT'mk_AC
Cuts at: 0 2620 6510
Size: 2620 3890
Acc65I G'GTAC_C
Cuts at: 0 1678 6510
Size: 1678 4832
AclI AA'CG_TT
Cuts at: 0 3629 6510
Size: 3629 2881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1307 2286 2597 3286 3727 4862 5645
Size: 798 509 979 311 689 441 1135 783
Cuts at: 5645 6011 6510
Size: 366 499
Fragments arranged by size:
1135 979 798 783 689 509 499 441
366 311
AfeI AGC'GCT
Cuts at: 0 3556 6510
Size: 3556 2954
AflII C'TTAA_G
Cuts at: 0 5860 6510
Size: 5860 650
AflIII A'CryG_T
Cuts at: 0 2440 6510
Size: 2440 4070
AleI CACnn'nnGTG
Cuts at: 0 5110 6510
Size: 5110 1400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2023 5516 5830 6510
Size: 2023 3493 314 680
Fragments arranged by size:
3493 2023 680 314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5768 5800 6510
Size: 5768 32 710
Fragments arranged by size:
5768 710 32
AlwNI CAG_nnn'CTG
Cuts at: 0 85 1771 2026 2350 3423 3621 3847 6003
Size: 85 1686 255 324 1073 198 226 2156
Cuts at: 6003 6169 6484 6510
Size: 166 315 26
Fragments arranged by size:
2156 1686 1073 324 315 255 226 198
166 85 26
ApaI G_GGCC'C
Cuts at: 0 20 6510
Size: 20 6490
ApaLI G'TGCA_C
Cuts at: 0 5866 6510
Size: 5866 644
ApoI r'AATT_y
Cuts at: 0 184 1093 1391 1432 2501 2745 3372 3765
Size: 184 909 298 41 1069 244 627 393
Cuts at: 3765 4442 4508 4688 5189 5435 5608 5680 6106
Size: 677 66 180 501 246 173 72 426
Cuts at: 6106 6510
Size: 404
Fragments arranged by size:
1069 909 677 627 501 426 404 393
298 246 244 184 180 173 72 66
41
AvaI C'yCGr_G
Cuts at: 0 462 1154 1363 3989 5078 6510
Size: 462 692 209 2626 1089 1432
Fragments arranged by size:
2626 1432 1089 692 462 209
BamHI G'GATC_C
Cuts at: 0 5010 6510
Size: 5010 1500
BanI G'GyrC_C
Cuts at: 0 1678 2713 3225 3507 4285 5026 6510
Size: 1678 1035 512 282 778 741 1484
Fragments arranged by size:
1678 1484 1035 778 741 512 282
BanII G_rGCy'C
Cuts at: 0 20 1718 2890 3956 4265 4724 5355 5909
Size: 20 1698 1172 1066 309 459 631 554
Cuts at: 5909 6510
Size: 601
Fragments arranged by size:
1698 1172 1066 631 601 554 459 309
20
BbeI G_GCGC'C
Cuts at: 0 4289 6510
Size: 4289 2221
BbsI GAAGACnn'nnnn_
Cuts at: 0 911 1313 2210 3614 4624 4957 5711 5826
Size: 911 402 897 1404 1010 333 754 115
Cuts at: 5826 6510
Size: 684
Fragments arranged by size:
1404 1010 911 897 754 684 402 333
115
BbvCI CC'TCA_GC
Cuts at: 0 4944 5171 6510
Size: 4944 227 1339
Fragments arranged by size:
4944 1339 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5072 5106 6510
Size: 5072 34 1404
Fragments arranged by size:
5072 1404 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5649 6510
Size: 5649 861
BclI T'GATC_A
Cuts at: 0 6336 6510
Size: 6336 174
BfrBI ATG'CAT
Cuts at: 0 2104 6510
Size: 2104 4406
BglI GCCn_nnn'nGGC
Cuts at: 0 24 3944 4415 6510
Size: 24 3920 471 2095
Fragments arranged by size:
3920 2095 471 24
BglII A'GATC_T
Cuts at: 0 5978 6510
Size: 5978 532
BlpI GC'TnA_GC
Cuts at: 0 1712 4571 6510
Size: 1712 2859 1939
Fragments arranged by size:
2859 1939 1712
Bme1580I G_kGCm'C
Cuts at: 0 20 469 3228 3512 5029 5870 6510
Size: 20 449 2759 284 1517 841 640
Fragments arranged by size:
2759 1517 841 640 449 284 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 2060 2885 3224 6207 6510
Size: 2060 825 339 2983 303
Fragments arranged by size:
2983 2060 825 339 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1970 2010 3137 6510
Size: 1970 40 1127 3373
Fragments arranged by size:
3373 1970 1127 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 617 1000 1965 3341 4036 4109 4354
Size: 505 112 383 965 1376 695 73 245
Cuts at: 4354 5339 6510
Size: 985 1171
Fragments arranged by size:
1376 1171 985 965 695 505 383 245
112 73
Bpu10I CC'TnA_GC
Cuts at: 0 3558 4560 4944 5171 6510
Size: 3558 1002 384 227 1339
Fragments arranged by size:
3558 1339 1002 384 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2253 2480 4126 6171 6510
Size: 2253 227 1646 2045 339
Fragments arranged by size:
2253 2045 1646 339 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 1157 2466 3582 3799 6481 6510
Size: 1157 1309 1116 217 2682 29
Fragments arranged by size:
2682 1309 1157 1116 217 29
BsaAI yAC'GTr
Cuts at: 0 2624 6510
Size: 2624 3886
BsaHI Gr'CG_yC
Cuts at: 0 4286 4697 6510
Size: 4286 411 1813
Fragments arranged by size:
4286 1813 411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 503 533 1849 1879 3995 4025 4821 4851
Size: 503 30 1316 30 2116 30 796 30
Cuts at: 4851 5768 5798 6095 6125 6510
Size: 917 30 297 30 385
Fragments arranged by size:
2116 1316 917 796 503 385 297 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 973 1251 1946 2050 3124 4468 4932
Size: 477 496 278 695 104 1074 1344 464
Cuts at: 4932 5140 5776 6103 6106 6510
Size: 208 636 327 3 404
Fragments arranged by size:
1344 1074 695 636 496 477 464 404
327 278 208 104 3
BseYI C'CCAG_C
Cuts at: 0 883 1044 1171 2029 2800 3054 3428 3598
Size: 883 161 127 858 771 254 374 170
Cuts at: 3598 4489 6381 6510
Size: 891 1892 129
Fragments arranged by size:
1892 891 883 858 771 374 254 170
161 129 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 3184 3431 3470 5512 5522 5752 6510
Size: 824 2360 247 39 2042 10 230 758
Fragments arranged by size:
2360 2042 824 758 247 230 39 10
BsiHKAI G_wGCw'C
Cuts at: 0 319 5355 5870 5909 6510
Size: 319 5036 515 39 601
Fragments arranged by size:
5036 601 515 319 39
BsmI GAATG_Cn'
Cuts at: 0 705 1653 5947 6510
Size: 705 948 4294 563
Fragments arranged by size:
4294 948 705 563
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1531 6510
Size: 1531 4979
Bsp1286I G_dGCh'C
Cuts at: 0 20 319 469 1718 2890 3228 3512 3956
Size: 20 299 150 1249 1172 338 284 444
Cuts at: 3956 4265 4724 5029 5355 5870 5909 6510
Size: 309 459 305 326 515 39 601
Fragments arranged by size:
1249 1172 601 515 459 444 338 326
309 305 299 284 150 39 20
BspHI T'CATG_A
Cuts at: 0 756 4692 5439 6510
Size: 756 3936 747 1071
Fragments arranged by size:
3936 1071 756 747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1049 3088 4189 6510
Size: 1049 2039 1101 2321
Fragments arranged by size:
2321 2039 1101 1049
BsrDI GCAATG_nn'
Cuts at: 0 49 1104 2414 3035 5876 6510
Size: 49 1055 1310 621 2841 634
Fragments arranged by size:
2841 1310 1055 634 621 49
BsrFI r'CCGG_y
Cuts at: 0 4344 6510
Size: 4344 2166
BsrGI T'GTAC_A
Cuts at: 0 3966 6510
Size: 3966 2544
BssSI C'ACGA_G
Cuts at: 0 2291 3122 6510
Size: 2291 831 3388
Fragments arranged by size:
3388 2291 831
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2404 4465 4482 6510
Size: 2404 2061 17 2028
Fragments arranged by size:
2404 2061 2028 17
BstEII G'GTnAC_C
Cuts at: 0 733 2428 6510
Size: 733 1695 4082
Fragments arranged by size:
4082 1695 733
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1228 4014 6510
Size: 1228 2786 2496
Fragments arranged by size:
2786 2496 1228
BstYI r'GATC_y
Cuts at: 0 4209 5010 5372 5978 6510
Size: 4209 801 362 606 532
Fragments arranged by size:
4209 801 606 532 362
Bsu36I CC'TnA_GG
Cuts at: 0 1958 3083 4451 6510
Size: 1958 1125 1368 2059
Fragments arranged by size:
2059 1958 1368 1125
BtgI C'CryG_G
Cuts at: 0 710 866 3512 3581 4025 5756 6510
Size: 710 156 2646 69 444 1731 754
Fragments arranged by size:
2646 1731 754 710 444 156 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1091 6510
Size: 1091 5419
BtsI GCAGTG_nn'
Cuts at: 0 1187 1355 2405 4154 4554 5117 5163 6510
Size: 1187 168 1050 1749 400 563 46 1347
Fragments arranged by size:
1749 1347 1187 1050 563 400 168 46
ClaI AT'CG_AT
Cuts at: 0 155 6510
Size: 155 6355
DraI TTT'AAA
Cuts at: 0 2868 4167 6309 6345 6510
Size: 2868 1299 2142 36 165
Fragments arranged by size:
2868 2142 1299 165 36
DraIII CAC_nnn'GTG
Cuts at: 0 3232 6201 6510
Size: 3232 2969 309
Fragments arranged by size:
3232 2969 309
DrdI GACnn_nn'nnGTC
Cuts at: 0 2601 2616 6510
Size: 2601 15 3894
Fragments arranged by size:
3894 2601 15
EaeI y'GGCC_r
Cuts at: 0 869 2781 3745 4346 5844 6510
Size: 869 1912 964 601 1498 666
Fragments arranged by size:
1912 1498 964 869 666 601
EarI CTCTTCn'nnn_
Cuts at: 0 1079 3305 3362 3653 3944 4806 5300 5343
Size: 1079 2226 57 291 291 862 494 43
Cuts at: 5343 5666 6150 6510
Size: 323 484 360
Fragments arranged by size:
2226 1079 862 494 484 360 323 291
291 57 43
EcoICRI GAG'CTC
Cuts at: 0 5353 5907 6510
Size: 5353 554 603
Fragments arranged by size:
5353 603 554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 617 798 1000 1307 1965 2286 2597
Size: 505 112 181 202 307 658 321 311
Cuts at: 2597 3286 3341 3727 4036 4109 4354 4862 5339
Size: 689 55 386 309 73 245 508 477
Cuts at: 5339 5645 6011 6510
Size: 306 366 499
Fragments arranged by size:
689 658 508 505 499 477 386 366
321 311 309 307 306 245 202 181
112 73 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6005 6510
Size: 6005 505
EcoO109I rG'GnC_Cy
Cuts at: 0 16 17 26 1300 5099 6481 6510
Size: 16 1 9 1274 3799 1382 29
Fragments arranged by size:
3799 1382 1274 29 16 9 1
EcoRI G'AATT_C
Cuts at: 0 184 1093 1391 2745 4508 5680 6510
Size: 184 909 298 1354 1763 1172 830
Fragments arranged by size:
1763 1354 1172 909 830 298 184
EcoRV GAT'ATC
Cuts at: 0 3648 6510
Size: 3648 2862
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1699 1763 3232 4001 6510
Size: 1699 64 1469 769 2509
Fragments arranged by size:
2509 1699 1469 769 64
HaeII r_GCGC'y
Cuts at: 0 3558 4289 6510
Size: 3558 731 2221
Fragments arranged by size:
3558 2221 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 1303 1334 1335 1366 1849 1881
Size: 304 32 967 31 1 31 483 32
Cuts at: 1881 1976 2008 2236 2268 2632 2664 2877 2909
Size: 95 32 228 32 364 32 213 32
Cuts at: 2909 3007 3039 3301 3333 3702 3734 3895 3927
Size: 98 32 262 32 369 32 161 32
Cuts at: 3927 4054 4086 5461 5493 6510
Size: 127 32 1375 32 1017
Fragments arranged by size:
1375 1017 967 483 369 364 304 262
228 213 161 127 98 95 32 32
32 32 32 32 32 32 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 4107 4665 6510
Size: 4107 558 1845
Fragments arranged by size:
4107 1845 558
HpaI GTT'AAC
Cuts at: 0 4107 6510
Size: 4107 2403
KasI G'GCGC_C
Cuts at: 0 4285 6510
Size: 4285 2225
KpnI G_GTAC'C
Cuts at: 0 1682 6510
Size: 1682 4828
MfeI C'AATT_G
Cuts at: 0 334 2939 6510
Size: 334 2605 3571
Fragments arranged by size:
3571 2605 334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 352 527 1756 3149 3483 6510
Size: 200 152 175 1229 1393 334 3027
Fragments arranged by size:
3027 1393 1229 334 200 175 152
MscI TGG'CCA
Cuts at: 0 871 2783 3747 5846 6510
Size: 871 1912 964 2099 664
Fragments arranged by size:
2099 1912 964 871 664
MslI CAynn'nnrTG
Cuts at: 0 547 668 1310 1790 3098 5110 5812 5852
Size: 547 121 642 480 1308 2012 702 40
Cuts at: 5852 6510
Size: 658
Fragments arranged by size:
2012 1308 702 658 642 547 480 121
40
MspA1I CmG'CkG
Cuts at: 0 33 481 1546 4075 5623 5640 6510
Size: 33 448 1065 2529 1548 17 870
Fragments arranged by size:
2529 1548 1065 870 448 33 17
NaeI GCC'GGC
Cuts at: 0 4346 6510
Size: 4346 2164
NarI GG'CG_CC
Cuts at: 0 4286 6510
Size: 4286 2224
NcoI C'CATG_G
Cuts at: 0 710 6510
Size: 710 5800
NgoMIV G'CCGG_C
Cuts at: 0 4344 6510
Size: 4344 2166
NsiI A_TGCA'T
Cuts at: 0 2106 6510
Size: 2106 4404
NspI r_CATG'y
Cuts at: 0 1060 1905 2779 3097 6243 6510
Size: 1060 845 874 318 3146 267
Fragments arranged by size:
3146 1060 874 845 318 267
PasI CC'CwG_GG
Cuts at: 0 5031 6510
Size: 5031 1479
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 2050 3339 4878 5158 6510
Size: 528 1522 1289 1539 280 1352
Fragments arranged by size:
1539 1522 1352 1289 528 280
PfoI T'CCnGG_A
Cuts at: 0 1386 4013 6510
Size: 1386 2627 2497
Fragments arranged by size:
2627 2497 1386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3100 3132 5768 5800 6510
Size: 3100 32 2636 32 710
Fragments arranged by size:
3100 2636 710 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1300 5099 6510
Size: 1300 3799 1411
Fragments arranged by size:
3799 1411 1300
PshAI GACnn'nnGTC
Cuts at: 0 3574 3791 6510
Size: 3574 217 2719
Fragments arranged by size:
3574 2719 217
PsiI TTA'TAA
Cuts at: 0 440 3715 6510
Size: 440 3275 2795
Fragments arranged by size:
3275 2795 440
PspOMI G'GGCC_C
Cuts at: 0 16 6510
Size: 16 6494
PstI C_TGCA'G
Cuts at: 0 3263 3289 5009 6510
Size: 3263 26 1720 1501
Fragments arranged by size:
3263 1720 1501 26
PvuII CAG'CTG
Cuts at: 0 481 1546 5623 5640 6510
Size: 481 1065 4077 17 870
Fragments arranged by size:
4077 1065 870 481 17
SacI G_AGCT'C
Cuts at: 0 5355 5909 6510
Size: 5355 554 601
Fragments arranged by size:
5355 601 554
SanDI GG'GwC_CC
Cuts at: 0 5099 6510
Size: 5099 1411
SapI GCTCTTCn'nnn_
Cuts at: 0 3944 5343 6510
Size: 3944 1399 1167
Fragments arranged by size:
3944 1399 1167
ScaI AGT'ACT
Cuts at: 0 2676 6510
Size: 2676 3834
SexAI A'CCwGG_T
Cuts at: 0 4816 6510
Size: 4816 1694
SfcI C'TryA_G
Cuts at: 0 2510 3259 3285 4034 4205 5005 6080 6510
Size: 2510 749 26 749 171 800 1075 430
Fragments arranged by size:
2510 1075 800 749 749 430 171 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 24 6510
Size: 24 6486
SfoI GGC'GCC
Cuts at: 0 4287 6510
Size: 4287 2223
SmaI CCC'GGG
Cuts at: 0 464 3991 6510
Size: 464 3527 2519
Fragments arranged by size:
3527 2519 464
SmlI C'TyrA_G
Cuts at: 0 2268 2495 4141 5860 6150 6510
Size: 2268 227 1646 1719 290 360
Fragments arranged by size:
2268 1719 1646 360 290 227
SphI G_CATG'C
Cuts at: 0 1905 3097 6510
Size: 1905 1192 3413
Fragments arranged by size:
3413 1905 1192
SspI AAT'ATT
Cuts at: 0 2488 6510
Size: 2488 4022
StyI C'CwwG_G
Cuts at: 0 710 2282 2383 2433 2735 2953 4955 6510
Size: 710 1572 101 50 302 218 2002 1555
Fragments arranged by size:
2002 1572 1555 710 302 218 101 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 562 1102 1106 3068 3243 6189 6510
Size: 562 540 4 1962 175 2946 321
Fragments arranged by size:
2946 1962 562 540 321 175 4
TatI w'GTAC_w
Cuts at: 0 795 1666 2674 2772 3966 4238 4828 6510
Size: 795 871 1008 98 1194 272 590 1682
Fragments arranged by size:
1682 1194 1008 871 795 590 272 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 503 535 2356 2388 3239 3271 3440 3472
Size: 503 32 1821 32 851 32 169 32
Cuts at: 3472 6095 6127 6510
Size: 2623 32 383
Fragments arranged by size:
2623 1821 851 503 383 169 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1861 2250 3811 5118 6510
Size: 1861 389 1561 1307 1392
Fragments arranged by size:
1861 1561 1392 1307 389
XbaI T'CTAG_A
Cuts at: 0 3720 6510
Size: 3720 2790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 128 2757 6214 6510
Size: 128 2629 3457 296
Fragments arranged by size:
3457 2629 296 128
XmaI C'CCGG_G
Cuts at: 0 462 3989 6510
Size: 462 3527 2521
Fragments arranged by size:
3527 2521 462
XmnI GAAnn'nnTTC
Cuts at: 0 1320 3981 4442 4508 5783 6510
Size: 1320 2661 461 66 1275 727
Fragments arranged by size:
2661 1320 1275 727 461 66
ZraI GAC'GTC
Cuts at: 0 4698 6510
Size: 4698 1812
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AscI AseI AsiSI AvrII BaeI BmgBI
BmtI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssHII
BstBI BstZ17I CspCI EagI EciI FseI FspI FspAI
HindIII MluI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PspXI PsrI PvuI RsrII SacII SalI
SbfI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
XhoI