Restriction map for
pFN21AE3713
(Linear) (Six-base) MAPSORT of: KIEE3713.seq Check: 7000 from: 1 to: 6075
KIEE3713.orf
With 182 enzymes: SgfI *
July 3, 2012 18:16 ..
AccI GT'mk_AC
Cuts at: 0 2420 4759 6075
Size: 2420 2339 1316
Fragments arranged by size:
2420 2339 1316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1688 2166 3107 3532 3665 4738 5020
Size: 901 787 478 941 425 133 1073 282
Cuts at: 5020 5228 5417 6075
Size: 208 189 658
Fragments arranged by size:
1073 941 901 787 658 478 425 282
208 189 133
AfeI AGC'GCT
Cuts at: 0 1524 5973 6075
Size: 1524 4449 102
Fragments arranged by size:
4449 1524 102
AflII C'TTAA_G
Cuts at: 0 2373 4552 6075
Size: 2373 2179 1523
Fragments arranged by size:
2373 2179 1523
AflIII A'CryG_T
Cuts at: 0 4010 4377 6075
Size: 4010 367 1698
Fragments arranged by size:
4010 1698 367
AgeI A'CCGG_T
Cuts at: 0 3891 6075
Size: 3891 2184
AhdI GACnn_n'nnGTC
Cuts at: 0 5049 6075
Size: 5049 1026
AleI CACnn'nnGTG
Cuts at: 0 5755 6075
Size: 5755 320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4406 6075
Size: 4406 1669
AlwNI CAG_nnn'CTG
Cuts at: 0 2999 3187 3208 3847 6054 6075
Size: 2999 188 21 639 2207 21
Fragments arranged by size:
2999 2207 639 188 21 21
ApaI G_GGCC'C
Cuts at: 0 954 6075
Size: 954 5121
ApoI r'AATT_y
Cuts at: 0 91 180 187 360 1279 1906 2056 2209
Size: 91 89 7 173 919 627 150 153
Cuts at: 2209 2456 2589 2974 3177 3235 3578 3637 3726
Size: 247 133 385 203 58 343 59 89
Cuts at: 3726 4317 4472 6075
Size: 591 155 1603
Fragments arranged by size:
1603 919 627 591 385 343 247 203
173 155 153 150 133 91 89 89
59 58 7
AseI AT'TA_AT
Cuts at: 0 1193 6075
Size: 1193 4882
AvaI C'yCGr_G
Cuts at: 0 5224 6075
Size: 5224 851
AvrII C'CTAG_G
Cuts at: 0 1120 6075
Size: 1120 4955
BamHI G'GATC_C
Cuts at: 0 1080 2717 5316 6075
Size: 1080 1637 2599 759
Fragments arranged by size:
2599 1637 1080 759
BanI G'GyrC_C
Cuts at: 0 4101 6075
Size: 4101 1974
BanII G_rGCy'C
Cuts at: 0 816 954 2702 3927 4228 5741 5779 6075
Size: 816 138 1748 1225 301 1513 38 296
Fragments arranged by size:
1748 1513 1225 816 301 296 138 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 638 1058 1816 2306 2567 3167 5234 6075
Size: 638 420 758 490 261 600 2067 841
Fragments arranged by size:
2067 841 758 638 600 490 420 261
BbvCI CC'TCA_GC
Cuts at: 0 14 6075
Size: 14 6061
BciVI GTATCCnnnnn_n'
Cuts at: 0 3780 3940 4935 6075
Size: 3780 160 995 1140
Fragments arranged by size:
3780 1140 995 160
BglII A'GATC_T
Cuts at: 0 4616 6075
Size: 4616 1459
Bme1580I G_kGCm'C
Cuts at: 0 954 5927 6075
Size: 954 4973 148
Fragments arranged by size:
4973 954 148
BmgBI CAC'GTC
Cuts at: 0 5286 5494 6075
Size: 5286 208 581
Fragments arranged by size:
5286 581 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 957 1703 4023 5863 6032 6075
Size: 957 746 2320 1840 169 43
Fragments arranged by size:
2320 1840 957 746 169 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5760 6075
Size: 5760 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 1916 5471 6075
Size: 1850 66 3555 604
Fragments arranged by size:
3555 1850 604 66
Bpu10I CC'TnA_GC
Cuts at: 0 14 6075
Size: 14 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 3517 3919 4240 6075
Size: 403 3114 402 321 1835
Fragments arranged by size:
3114 1835 403 402 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 3825 4561 6075
Size: 3825 736 1514
Fragments arranged by size:
3825 1514 736
BsaAI yAC'GTr
Cuts at: 0 66 346 3956 4380 6075
Size: 66 280 3610 424 1695
Fragments arranged by size:
3610 1695 424 280 66
BsaBI GATnn'nnATC
Cuts at: 0 2176 6075
Size: 2176 3899
BsaHI Gr'CG_yC
Cuts at: 0 332 6075
Size: 332 5743
BsaWI w'CCGG_w
Cuts at: 0 3891 6075
Size: 3891 2184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1396 1426 6075
Size: 1396 30 4649
Fragments arranged by size:
4649 1396 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2380 6075
Size: 2380 3695
BseYI C'CCAG_C
Cuts at: 0 933 2949 5007 5740 6075
Size: 933 2016 2058 733 335
Fragments arranged by size:
2058 2016 933 733 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1511 4379 4402 5200 6075
Size: 1418 93 2868 23 798 875
Fragments arranged by size:
2868 1418 875 798 93 23
BsiHKAI G_wGCw'C
Cuts at: 0 816 2557 4310 4496 5549 5915 6075
Size: 816 1741 1753 186 1053 366 160
Fragments arranged by size:
1753 1741 1053 816 366 186 160
BsmI GAATG_Cn'
Cuts at: 0 1924 4176 6075
Size: 1924 2252 1899
Fragments arranged by size:
2252 1924 1899
Bsp1286I G_dGCh'C
Cuts at: 0 816 954 2557 2702 3927 4228 4310 4496
Size: 816 138 1603 145 1225 301 82 186
Cuts at: 4496 5549 5741 5779 5915 5927 6075
Size: 1053 192 38 136 12 148
Fragments arranged by size:
1603 1225 1053 816 301 192 186 148
145 138 136 82 38 12
BspHI T'CATG_A
Cuts at: 0 2807 6075
Size: 2807 3268
BsrDI GCAATG_nn'
Cuts at: 0 108 497 2953 6075
Size: 108 389 2456 3122
Fragments arranged by size:
3122 2456 389 108
BsrFI r'CCGG_y
Cuts at: 0 3891 6075
Size: 3891 2184
BssSI C'ACGA_G
Cuts at: 0 1848 5881 6075
Size: 1848 4033 194
Fragments arranged by size:
4033 1848 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5857 6075
Size: 5857 218
BstBI TT'CG_AA
Cuts at: 0 3239 6075
Size: 3239 2836
BstEII G'GTnAC_C
Cuts at: 0 805 4189 6075
Size: 805 3384 1886
Fragments arranged by size:
3384 1886 805
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3920 4578 6075
Size: 3920 658 1497
Fragments arranged by size:
3920 1497 658
BstYI r'GATC_y
Cuts at: 0 1011 1080 1560 1982 2321 2717 4214 4616
Size: 1011 69 480 422 339 396 1497 402
Cuts at: 4616 5316 6075
Size: 700 759
Fragments arranged by size:
1497 1011 759 700 480 422 402 396
339 69
BstZ17I GTA'TAC
Cuts at: 0 4760 6075
Size: 4760 1315
Bsu36I CC'TnA_GG
Cuts at: 0 998 1655 6075
Size: 998 657 4420
Fragments arranged by size:
4420 998 657
BtgI C'CryG_G
Cuts at: 0 1968 6075
Size: 1968 4107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 260 542 6075
Size: 260 282 5533
Fragments arranged by size:
5533 282 260
BtsI GCAGTG_nn'
Cuts at: 0 2193 2612 3209 4251 5959 6075
Size: 2193 419 597 1042 1708 116
Fragments arranged by size:
2193 1708 1042 597 419 116
DraI TTT'AAA
Cuts at: 0 3455 3642 6075
Size: 3455 187 2433
Fragments arranged by size:
3455 2433 187
DraIII CAC_nnn'GTG
Cuts at: 0 856 5857 5886 6075
Size: 856 5001 29 189
Fragments arranged by size:
5001 856 189 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 6012 6075
Size: 6012 63
EaeI y'GGCC_r
Cuts at: 0 271 892 1205 1589 4055 5256 5430 6075
Size: 271 621 313 384 2466 1201 174 645
Fragments arranged by size:
2466 1201 645 621 384 313 271 174
EarI CTCTTCn'nnn_
Cuts at: 0 713 1862 2308 2396 3828 4559 4577 4853
Size: 713 1149 446 88 1432 731 18 276
Cuts at: 4853 4886 6005 6021 6072 6075
Size: 33 1119 16 51 3
Fragments arranged by size:
1432 1149 1119 731 713 446 276 88
51 33 18 16 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1252 6075
Size: 49 1203 4823
Fragments arranged by size:
4823 1203 49
EcoICRI GAG'CTC
Cuts at: 0 814 6075
Size: 814 5261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1688 1850 1916 2166 3107 3532 3665
Size: 901 787 162 66 250 941 425 133
Cuts at: 3665 4738 5020 5228 5417 5471 6075
Size: 1073 282 208 189 54 604
Fragments arranged by size:
1073 941 901 787 604 425 282 250
208 189 162 133 66 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3839 6075
Size: 3839 2236
EcoO109I rG'GnC_Cy
Cuts at: 0 951 1109 1716 4575 5765 6075
Size: 951 158 607 2859 1190 310
Fragments arranged by size:
2859 1190 951 607 310 158
EcoRI G'AATT_C
Cuts at: 0 2456 2974 3235 6075
Size: 2456 518 261 2840
Fragments arranged by size:
2840 2456 518 261
EcoRV GAT'ATC
Cuts at: 0 3981 6075
Size: 3981 2094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1416 2551 4531 6075
Size: 1416 1135 1980 1544
Fragments arranged by size:
1980 1544 1416 1135
HaeII r_GCGC'y
Cuts at: 0 1526 5975 6075
Size: 1526 4449 100
Fragments arranged by size:
4449 1526 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1648 1680 1806 1838 2863 2875 2895 2907
Size: 1648 32 126 32 1025 12 20 12
Cuts at: 2907 3325 3357 3640 3672 3965 3997 5035 5067
Size: 418 32 283 32 293 32 1038 32
Cuts at: 5067 5536 5568 6075
Size: 469 32 507
Fragments arranged by size:
1648 1038 1025 507 469 418 293 283
126 32 32 32 32 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 1838 6075
Size: 1838 4237
HindIII A'AGCT_T
Cuts at: 0 1473 6075
Size: 1473 4602
HpaI GTT'AAC
Cuts at: 0 1838 6075
Size: 1838 4237
MfeI C'AATT_G
Cuts at: 0 557 564 1579 6075
Size: 557 7 1015 4496
Fragments arranged by size:
4496 1015 557 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3797 6075
Size: 3797 2278
MscI TGG'CCA
Cuts at: 0 273 894 1207 1591 4057 5258 5432 6075
Size: 273 621 313 384 2466 1201 174 643
Fragments arranged by size:
2466 1201 643 621 384 313 273 174
MslI CAynn'nnrTG
Cuts at: 0 68 323 4009 4169 5755 6075
Size: 68 255 3686 160 1586 320
Fragments arranged by size:
3686 1586 320 255 160 68
MspA1I CmG'CkG
Cuts at: 0 2033 2999 3628 5409 6075
Size: 2033 966 629 1781 666
Fragments arranged by size:
2033 1781 966 666 629
NcoI C'CATG_G
Cuts at: 0 1968 6075
Size: 1968 4107
NspI r_CATG'y
Cuts at: 0 1030 2674 6075
Size: 1030 1644 3401
Fragments arranged by size:
3401 1644 1030
PasI CC'CwG_GG
Cuts at: 0 5347 5772 6075
Size: 5347 425 303
Fragments arranged by size:
5347 425 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 5386 6075
Size: 5386 689
PfoI T'CCnGG_A
Cuts at: 0 1827 5487 6075
Size: 1827 3660 588
Fragments arranged by size:
3660 1827 588
PmlI CAC'GTG
Cuts at: 0 66 346 4380 6075
Size: 66 280 4034 1695
Fragments arranged by size:
4034 1695 280 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5617 5649 6075
Size: 5617 32 426
Fragments arranged by size:
5617 426 32
PpuMI rG'GwC_Cy
Cuts at: 0 4575 5765 6075
Size: 4575 1190 310
Fragments arranged by size:
4575 1190 310
PspOMI G'GGCC_C
Cuts at: 0 950 6075
Size: 950 5125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2411 2443 4781 4813 6075
Size: 2411 32 2338 32 1262
Fragments arranged by size:
2411 2338 1262 32 32
PstI C_TGCA'G
Cuts at: 0 202 3192 3399 4258 4920 5034 6075
Size: 202 2990 207 859 662 114 1041
Fragments arranged by size:
2990 1041 859 662 207 202 114
PvuII CAG'CTG
Cuts at: 0 2033 3628 5409 6075
Size: 2033 1595 1781 666
Fragments arranged by size:
2033 1781 1595 666
SacI G_AGCT'C
Cuts at: 0 816 6075
Size: 816 5259
SapI GCTCTTCn'nnn_
Cuts at: 0 2396 6072 6075
Size: 2396 3676 3
Fragments arranged by size:
3676 2396 3
ScaI AGT'ACT
Cuts at: 0 2044 6075
Size: 2044 4031
SexAI A'CCwGG_T
Cuts at: 0 4017 5761 6075
Size: 4017 1744 314
Fragments arranged by size:
4017 1744 314
SfcI C'TryA_G
Cuts at: 0 198 2417 2461 2852 3188 3395 4254 4916
Size: 198 2219 44 391 336 207 859 662
Cuts at: 4916 4966 5030 6075
Size: 50 64 1045
Fragments arranged by size:
2219 1045 859 662 391 336 207 198
64 50 44
SmlI C'TyrA_G
Cuts at: 0 382 2373 3532 3898 4219 4552 6075
Size: 382 1991 1159 366 321 333 1523
Fragments arranged by size:
1991 1523 1159 382 366 333 321
SnaBI TAC'GTA
Cuts at: 0 3956 6075
Size: 3956 2119
SspI AAT'ATT
Cuts at: 0 2663 6075
Size: 2663 3412
StuI AGG'CCT
Cuts at: 0 1534 6075
Size: 1534 4541
StyI C'CwwG_G
Cuts at: 0 1120 1638 1968 3050 3368 4052 5259 5813
Size: 1120 518 330 1082 318 684 1207 554
Cuts at: 5813 6075
Size: 262
Fragments arranged by size:
1207 1120 1082 684 554 518 330 318
262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 2406 4000 5820 5845 6075
Size: 133 2273 1594 1820 25 230
Fragments arranged by size:
2273 1820 1594 230 133 25
TatI w'GTAC_w
Cuts at: 0 2042 3001 4031 6075
Size: 2042 959 1030 2044
Fragments arranged by size:
2044 2042 1030 959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4935 4967 5205 5237 6075
Size: 4935 32 238 32 838
Fragments arranged by size:
4935 838 238 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5763 6075
Size: 5763 312
XbaI T'CTAG_A
Cuts at: 0 1776 3784 5682 6075
Size: 1776 2008 1898 393
Fragments arranged by size:
2008 1898 1776 393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4771 6075
Size: 4771 1304
XmnI GAAnn'nnTTC
Cuts at: 0 2237 3120 4340 4994 5075 5678 6075
Size: 2237 883 1220 654 81 603 397
Fragments arranged by size:
2237 1220 883 654 603 397 81
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApoI AseI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BciVI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SapI ScaI SexAI SfcI SmlI
SnaBI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AloI ApaLI AscI AsiSI
BaeI BbeI BcgI BclI BfrBI BglI BlpI BmtI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII
ClaI CspCI EagI FseI FspI FspAI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspXI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SwaI XhoI XmaI
ZraI