Restriction map for
pFN21AE3695
(Linear) (Six-base) MAPSORT of: KIEE3695.seq Check: 8965 from: 1 to: 2004
KIEE3695.orf
With 182 enzymes: SgfI *
March 22, 2012 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 130 2004
Size: 130 1874
Acc65I G'GTAC_C
Cuts at: 0 1028 2004
Size: 1028 976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 2004
Size: 229 1775
AflIII A'CryG_T
Cuts at: 0 288 290 593 2004
Size: 288 2 303 1411
Fragments arranged by size:
1411 303 288 2
AhdI GACnn_n'nnGTC
Cuts at: 0 966 2004
Size: 966 1038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 2004
Size: 967 1037
AlwNI CAG_nnn'CTG
Cuts at: 0 672 735 963 1899 2004
Size: 672 63 228 936 105
Fragments arranged by size:
936 672 228 105 63
ApaI G_GGCC'C
Cuts at: 0 53 2004
Size: 53 1951
ApaLI G'TGCA_C
Cuts at: 0 1651 2004
Size: 1651 353
AvaI C'yCGr_G
Cuts at: 0 550 1032 1283 2004
Size: 550 482 251 721
Fragments arranged by size:
721 550 482 251
BanI G'GyrC_C
Cuts at: 0 1028 1182 1243 1416 2004
Size: 1028 154 61 173 588
Fragments arranged by size:
1028 588 173 154 61
BanII G_rGCy'C
Cuts at: 0 53 308 639 830 1366 1465 1633 2004
Size: 53 255 331 191 536 99 168 371
Fragments arranged by size:
536 371 331 255 191 168 99 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 2004
Size: 73 1931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1900 1934 2004
Size: 1900 34 70
Fragments arranged by size:
1900 70 34
BglI GCCn_nnn'nGGC
Cuts at: 0 57 1191 1370 2004
Size: 57 1134 179 634
Fragments arranged by size:
1134 634 179 57
BglII A'GATC_T
Cuts at: 0 1598 2004
Size: 1598 406
BlpI GC'TnA_GC
Cuts at: 0 163 1156 1900 2004
Size: 163 993 744 104
Fragments arranged by size:
993 744 163 104
Bme1580I G_kGCm'C
Cuts at: 0 53 1421 1655 2004
Size: 53 1368 234 349
Fragments arranged by size:
1368 349 234 53
BmgBI CAC'GTC
Cuts at: 0 231 594 903 2004
Size: 231 363 309 1101
Fragments arranged by size:
1101 363 309 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 934 1667 2004
Size: 934 733 337
Fragments arranged by size:
934 733 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 694 904 1443 1603 2004
Size: 82 612 210 539 160 401
Fragments arranged by size:
612 539 401 210 160 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 511 932 1232 1873 2004
Size: 511 421 300 641 131
Fragments arranged by size:
641 511 421 300 131
BsaAI yAC'GTr
Cuts at: 0 291 2004
Size: 291 1713
BsaBI GATnn'nnATC
Cuts at: 0 202 2004
Size: 202 1802
BsaHI Gr'CG_yC
Cuts at: 0 340 487 608 761 1501 2004
Size: 340 147 121 153 740 503
Fragments arranged by size:
740 503 340 153 147 121
BsaWI w'CCGG_w
Cuts at: 0 1016 2004
Size: 1016 988
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 1159 1189 1498 1528 2004
Size: 388 30 741 30 309 30 476
Fragments arranged by size:
741 476 388 309 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 988 991 997 1239 1522 1606 2004
Size: 988 3 6 242 283 84 398
Fragments arranged by size:
988 398 283 242 84 6 3
BseYI C'CCAG_C
Cuts at: 0 72 102 877 1211 1402 1852 1892 1925
Size: 72 30 775 334 191 450 40 33
Cuts at: 1925 2004
Size: 79
Fragments arranged by size:
775 450 334 191 79 72 40 33
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 880 2004
Size: 226 654 1124
Fragments arranged by size:
1124 654 226
BsiEI CG_ry'CG
Cuts at: 0 582 2004
Size: 582 1422
BsiHKAI G_wGCw'C
Cuts at: 0 224 308 788 1157 1633 1655 1906 2004
Size: 224 84 480 369 476 22 251 98
Fragments arranged by size:
480 476 369 251 224 98 84 22
Bsp1286I G_dGCh'C
Cuts at: 0 53 224 308 639 788 830 1157 1366
Size: 53 171 84 331 149 42 327 209
Cuts at: 1366 1421 1465 1633 1655 1906 2004
Size: 55 44 168 22 251 98
Fragments arranged by size:
331 327 251 209 171 168 149 98
84 55 53 44 42 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 486 971 1061 2004
Size: 130 356 485 90 943
Fragments arranged by size:
943 485 356 130 90
BsrBI CCG'CTC
Cuts at: 0 192 869 2004
Size: 192 677 1135
Fragments arranged by size:
1135 677 192
BsrFI r'CCGG_y
Cuts at: 0 56 1112 1755 2004
Size: 56 1056 643 249
Fragments arranged by size:
1056 643 249 56
BssHII G'CGCG_C
Cuts at: 0 26 2004
Size: 26 1978
BstAPI GCAn_nnn'nTGC
Cuts at: 0 963 2004
Size: 963 1041
BstEII G'GTnAC_C
Cuts at: 0 1287 2004
Size: 1287 717
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1206 2004
Size: 1206 798
BstYI r'GATC_y
Cuts at: 0 420 863 1598 2004
Size: 420 443 735 406
Fragments arranged by size:
735 443 420 406
Bsu36I CC'TnA_GG
Cuts at: 0 344 1251 2004
Size: 344 907 753
Fragments arranged by size:
907 753 344
BtgI C'CryG_G
Cuts at: 0 1145 1727 2004
Size: 1145 582 277
Fragments arranged by size:
1145 582 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 453 2004
Size: 453 1551
BtsI GCAGTG_nn'
Cuts at: 0 111 2004
Size: 111 1893
DrdI GACnn_nn'nnGTC
Cuts at: 0 1530 2004
Size: 1530 474
EaeI y'GGCC_r
Cuts at: 0 134 579 1129 1218 2004
Size: 134 445 550 89 786
Fragments arranged by size:
786 550 445 134 89
EagI C'GGCC_G
Cuts at: 0 579 2004
Size: 579 1425
EarI CTCTTCn'nnn_
Cuts at: 0 313 2004
Size: 313 1691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 2004
Size: 604 1400
EcoICRI GAG'CTC
Cuts at: 0 306 1631 2004
Size: 306 1325 373
Fragments arranged by size:
1325 373 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 229 694 904 1443 1603 2004
Size: 82 147 465 210 539 160 401
Fragments arranged by size:
539 465 401 210 160 147 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1305 1585 2004
Size: 1305 280 419
Fragments arranged by size:
1305 419 280
EcoO109I rG'GnC_Cy
Cuts at: 0 50 530 960 1050 1849 2004
Size: 50 480 430 90 799 155
Fragments arranged by size:
799 480 430 155 90 50
EcoRV GAT'ATC
Cuts at: 0 1776 2004
Size: 1776 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1470 2004
Size: 1470 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1062 1094 1159 1191 1498 1530
Size: 952 32 78 32 65 32 307 32
Cuts at: 1530 1659 1691 2004
Size: 129 32 313
Fragments arranged by size:
952 313 307 129 78 65 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 150 2004
Size: 150 1854
KpnI G_GTAC'C
Cuts at: 0 1032 2004
Size: 1032 972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1389 1522 1541 2004
Size: 494 895 133 19 463
Fragments arranged by size:
895 494 463 133 19
MscI TGG'CCA
Cuts at: 0 136 2004
Size: 136 1868
MslI CAynn'nnrTG
Cuts at: 0 1150 1204 2004
Size: 1150 54 800
Fragments arranged by size:
1150 800 54
MspA1I CmG'CkG
Cuts at: 0 524 669 1241 1896 2004
Size: 524 145 572 655 108
Fragments arranged by size:
655 572 524 145 108
NcoI C'CATG_G
Cuts at: 0 1145 2004
Size: 1145 859
NdeI CA'TA_TG
Cuts at: 0 409 802 2004
Size: 409 393 1202
Fragments arranged by size:
1202 409 393
NruI TCG'CGA
Cuts at: 0 202 2004
Size: 202 1802
NspI r_CATG'y
Cuts at: 0 705 918 2004
Size: 705 213 1086
Fragments arranged by size:
1086 705 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 2004
Size: 132 1872
PfoI T'CCnGG_A
Cuts at: 0 1010 2004
Size: 1010 994
PmlI CAC'GTG
Cuts at: 0 291 2004
Size: 291 1713
PpuMI rG'GwC_Cy
Cuts at: 0 530 960 1050 2004
Size: 530 430 90 954
Fragments arranged by size:
954 530 430 90
PshAI GACnn'nnGTC
Cuts at: 0 1342 2004
Size: 1342 662
PspOMI G'GGCC_C
Cuts at: 0 49 2004
Size: 49 1955
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1804 1836 2004
Size: 1804 32 168
Fragments arranged by size:
1804 168 32
PstI C_TGCA'G
Cuts at: 0 497 959 2004
Size: 497 462 1045
Fragments arranged by size:
1045 497 462
PvuII CAG'CTG
Cuts at: 0 524 669 1896 2004
Size: 524 145 1227 108
Fragments arranged by size:
1227 524 145 108
SacI G_AGCT'C
Cuts at: 0 308 1633 2004
Size: 308 1325 371
Fragments arranged by size:
1325 371 308
SanDI GG'GwC_CC
Cuts at: 0 530 2004
Size: 530 1474
SapI GCTCTTCn'nnn_
Cuts at: 0 313 2004
Size: 313 1691
SbfI CC_TGCA'GG
Cuts at: 0 497 2004
Size: 497 1507
SexAI A'CCwGG_T
Cuts at: 0 275 317 2004
Size: 275 42 1687
Fragments arranged by size:
1687 275 42
SfcI C'TryA_G
Cuts at: 0 493 955 2004
Size: 493 462 1049
Fragments arranged by size:
1049 493 462
SmaI CCC'GGG
Cuts at: 0 1285 2004
Size: 1285 719
SphI G_CATG'C
Cuts at: 0 918 2004
Size: 918 1086
SspI AAT'ATT
Cuts at: 0 268 2004
Size: 268 1736
StuI AGG'CCT
Cuts at: 0 1256 2004
Size: 1256 748
StyI C'CwwG_G
Cuts at: 0 1145 1306 1331 2004
Size: 1145 161 25 673
Fragments arranged by size:
1145 673 161 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1220 2004
Size: 1220 784
TatI w'GTAC_w
Cuts at: 0 1718 2004
Size: 1718 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1457 1489 2004
Size: 388 32 1037 32 515
Fragments arranged by size:
1037 515 388 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1950 2004
Size: 1950 54
XmaI C'CCGG_G
Cuts at: 0 1283 2004
Size: 1283 721
XmnI GAAnn'nnTTC
Cuts at: 0 361 2004
Size: 361 1643
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
NdeI NruI NspI PflMI PfoI PmlI PpuMI PshAI
PspOMI PsrI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SmaI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BmtI BplI Bpu10I BpuEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NsiI PacI PasI
PciI PmeI PpiI PsiI PspXI PvuI RsrII SacII
SalI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SwaI Tth111I XbaI XhoI ZraI