(Linear) (Six-base) MAPSORT of: KIEE3694.seq  Check: 9985  from: 1  to: 276



KIEE3694.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:15  ..

AflIII A'CryG_T

Cuts at:      0     254     276 
   Size:        254      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     170     276 
   Size:        170     106
 
BanII G_rGCy'C

Cuts at:      0      82     276 
   Size:         82     194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     276 
   Size:        224      52
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     276 
   Size:        151     125
 
BsaAI yAC'GTr

Cuts at:      0     255     276 
   Size:        255      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271     276 
   Size:        241      30       5

  Fragments arranged by size: 

                 241      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     276 
   Size:         19     257
 
BseYI C'CCAG_C

Cuts at:      0     176     276 
   Size:        176     100
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     276 
   Size:         82     194
 
BssSI C'ACGA_G

Cuts at:      0      31     276 
   Size:         31     245
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     170     276 
   Size:        170     106
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      21     276 
   Size:         21     255
 
HindIII A'AGCT_T

Cuts at:      0     124     276 
   Size:        124     152
 
MspA1I CmG'CkG

Cuts at:      0     184     276 
   Size:        184      92
 
PfoI T'CCnGG_A

Cuts at:      0     245     276 
   Size:        245      31
 
PvuII CAG'CTG

Cuts at:      0     184     276 
   Size:        184      92
 

 Enzymes that do cut:

   AflIII    AlwNI    BanII     BmrI     BsaI    BsaAI    BsaXI    BseRI
    BseYI Bsp1286I    BssSI   BstAPI     DrdI  HindIII   MspA1I     PfoI
    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI