Restriction map for
pFN21AE3691
(Linear) (Six-base) MAPSORT of: KIEE3691.seq Check: 1840 from: 1 to: 1965
KIEE3691.orf
With 182 enzymes: SgfI *
March 22, 2012 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1345 1965
Size: 1345 620
Acc65I G'GTAC_C
Cuts at: 0 1136 1965
Size: 1136 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1218 1617 1965
Size: 1218 399 348
Fragments arranged by size:
1218 399 348
AflII C'TTAA_G
Cuts at: 0 745 1965
Size: 745 1220
AleI CACnn'nnGTG
Cuts at: 0 1307 1965
Size: 1307 658
AlwNI CAG_nnn'CTG
Cuts at: 0 52 528 1056 1965
Size: 52 476 528 909
Fragments arranged by size:
909 528 476 52
ApoI r'AATT_y
Cuts at: 0 40 663 927 1164 1965
Size: 40 623 264 237 801
Fragments arranged by size:
801 623 264 237 40
AseI AT'TA_AT
Cuts at: 0 832 878 1965
Size: 832 46 1087
Fragments arranged by size:
1087 832 46
BamHI G'GATC_C
Cuts at: 0 1544 1965
Size: 1544 421
BanI G'GyrC_C
Cuts at: 0 1136 1554 1965
Size: 1136 418 411
Fragments arranged by size:
1136 418 411
BanII G_rGCy'C
Cuts at: 0 25 770 875 1430 1514 1965
Size: 25 745 105 555 84 451
Fragments arranged by size:
745 555 451 105 84 25
BbeI G_GCGC'C
Cuts at: 0 1558 1965
Size: 1558 407
BbsI GAAGACnn'nnnn_
Cuts at: 0 122 170 1224 1743 1965
Size: 122 48 1054 519 222
Fragments arranged by size:
1054 519 222 122 48
BbvCI CC'TCA_GC
Cuts at: 0 1045 1965
Size: 1045 920
BlpI GC'TnA_GC
Cuts at: 0 679 1965
Size: 679 1286
Bme1580I G_kGCm'C
Cuts at: 0 1314 1965
Size: 1314 651
BmrI ACTGGGnnnn_n'
Cuts at: 0 40 1965
Size: 40 1925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1624 1965
Size: 1624 341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1965
Size: 71 1894
Bpu10I CC'TnA_GC
Cuts at: 0 1045 1663 1965
Size: 1045 618 302
Fragments arranged by size:
1045 618 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1965
Size: 784 1181
BsaAI yAC'GTr
Cuts at: 0 885 1965
Size: 885 1080
BsaHI Gr'CG_yC
Cuts at: 0 1555 1965
Size: 1555 410
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 9 39 1501 1531 1965
Size: 9 30 1462 30 434
Fragments arranged by size:
1462 434 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1052 1475 1965
Size: 1052 423 490
Fragments arranged by size:
1052 490 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1837 1943 1965
Size: 1837 106 22
Fragments arranged by size:
1837 106 22
BsiHKAI G_wGCw'C
Cuts at: 0 25 685 770 875 1514 1965
Size: 25 660 85 105 639 451
Fragments arranged by size:
660 639 451 105 85 25
BsmI GAATG_Cn'
Cuts at: 0 1883 1965
Size: 1883 82
Bsp1286I G_dGCh'C
Cuts at: 0 25 685 770 875 1314 1430 1514 1965
Size: 25 660 85 105 439 116 84 451
Fragments arranged by size:
660 451 439 116 105 85 84 25
BspHI T'CATG_A
Cuts at: 0 329 902 1491 1965
Size: 329 573 589 474
Fragments arranged by size:
589 573 474 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1345 1965
Size: 1345 620
BsrDI GCAATG_nn'
Cuts at: 0 673 1581 1965
Size: 673 908 384
Fragments arranged by size:
908 673 384
BsrGI T'GTAC_A
Cuts at: 0 1591 1965
Size: 1591 374
BssSI C'ACGA_G
Cuts at: 0 103 1965
Size: 103 1862
BstBI TT'CG_AA
Cuts at: 0 1754 1965
Size: 1754 211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 11 1023 1965
Size: 11 1012 942
Fragments arranged by size:
1012 942 11
BstYI r'GATC_y
Cuts at: 0 1544 1965
Size: 1544 421
BtgI C'CryG_G
Cuts at: 0 1121 1965
Size: 1121 844
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1134 1965
Size: 1134 831
DraI TTT'AAA
Cuts at: 0 1280 1965
Size: 1280 685
EaeI y'GGCC_r
Cuts at: 0 1437 1965
Size: 1437 528
EarI CTCTTCn'nnn_
Cuts at: 0 104 797 1965
Size: 104 693 1168
Fragments arranged by size:
1168 693 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1199 1965
Size: 1199 766
EcoICRI GAG'CTC
Cuts at: 0 23 768 873 1512 1965
Size: 23 745 105 639 453
Fragments arranged by size:
745 639 453 105 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1218 1617 1965
Size: 71 1147 399 348
Fragments arranged by size:
1147 399 348 71
EcoO109I rG'GnC_Cy
Cuts at: 0 190 1965
Size: 190 1775
EcoRV GAT'ATC
Cuts at: 0 1324 1965
Size: 1324 641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 1311 1965
Size: 286 1025 654
Fragments arranged by size:
1025 654 286
FspI TGC'GCA
Cuts at: 0 1078 1965
Size: 1078 887
HaeII r_GCGC'y
Cuts at: 0 1558 1965
Size: 1558 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1965
Size: 1093 32 840
Fragments arranged by size:
1093 840 32
HincII GTy'rAC
Cuts at: 0 74 645 1965
Size: 74 571 1320
Fragments arranged by size:
1320 571 74
HindIII A'AGCT_T
Cuts at: 0 466 1965
Size: 466 1499
HpaI GTT'AAC
Cuts at: 0 645 1965
Size: 645 1320
KasI G'GCGC_C
Cuts at: 0 1554 1965
Size: 1554 411
KpnI G_GTAC'C
Cuts at: 0 1140 1965
Size: 1140 825
MfeI C'AATT_G
Cuts at: 0 1706 1965
Size: 1706 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1965
Size: 576 1389
MscI TGG'CCA
Cuts at: 0 1439 1965
Size: 1439 526
MslI CAynn'nnrTG
Cuts at: 0 9 1307 1965
Size: 9 1298 658
Fragments arranged by size:
1298 658 9
MspA1I CmG'CkG
Cuts at: 0 1056 1965
Size: 1056 909
NarI GG'CG_CC
Cuts at: 0 1555 1965
Size: 1555 410
NcoI C'CATG_G
Cuts at: 0 1121 1965
Size: 1121 844
NspI r_CATG'y
Cuts at: 0 1083 1590 1965
Size: 1083 507 375
Fragments arranged by size:
1083 507 375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 784 1965
Size: 752 32 1181
Fragments arranged by size:
1181 752 32
PpuMI rG'GwC_Cy
Cuts at: 0 190 1965
Size: 190 1775
PshAI GACnn'nnGTC
Cuts at: 0 1567 1965
Size: 1567 398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 900 932 1965
Size: 900 32 1033
Fragments arranged by size:
1033 900 32
PvuII CAG'CTG
Cuts at: 0 1056 1965
Size: 1056 909
SacI G_AGCT'C
Cuts at: 0 25 770 875 1514 1965
Size: 25 745 105 639 451
Fragments arranged by size:
745 639 451 105 25
SapI GCTCTTCn'nnn_
Cuts at: 0 797 1965
Size: 797 1168
SfcI C'TryA_G
Cuts at: 0 520 1965
Size: 520 1445
SfoI GGC'GCC
Cuts at: 0 1556 1965
Size: 1556 409
SmlI C'TyrA_G
Cuts at: 0 745 763 1965
Size: 745 18 1202
Fragments arranged by size:
1202 745 18
StyI C'CwwG_G
Cuts at: 0 138 182 1121 1132 1875 1965
Size: 138 44 939 11 743 90
Fragments arranged by size:
939 743 138 90 44 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 1965
Size: 418 1547
TatI w'GTAC_w
Cuts at: 0 754 1591 1965
Size: 754 837 374
Fragments arranged by size:
837 754 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 1893 1925 1965
Size: 1501 32 360 32 40
Fragments arranged by size:
1501 360 40 32 32
XbaI T'CTAG_A
Cuts at: 0 1939 1965
Size: 1939 26
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlwNI ApoI AseI
BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BssSI BstBI BstXI BstYI BtgI BtgZI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NspI
PpiI PpuMI PshAI PsrI PvuII SacI SapI SfcI
SfoI SmlI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BsaI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BstAPI BstEII BstZ17I Bsu36I BtsI
ClaI CspCI DraIII DrdI EagI EcoNI EcoRI FseI
FspAI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XcmI XhoI XmaI XmnI ZraI